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Atp a095 1 1

Manufactured by Nanjing Jiancheng
Sourced in China

ATP (A095-1-1) is a lab equipment product that provides a source of adenosine triphosphate (ATP). ATP is a fundamental molecule involved in energy transfer and cellular processes. The product provides a consistent and reliable supply of ATP for various experimental and research applications.

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2 protocols using atp a095 1 1

1

Metabolic Analysis of Cell Samples

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Glucose (A154-1-1), lactate (A020-2-2) and ATP (A095-1-1) kits were purchased from Nanjing Jiancheng Biological Engineering Institute Co., LTD. Under the same treatment conditions, after all groups of cell samples were collected, cleaned and broken in sequence, glucose, lactate and ATP production were tested in accordance with the instructions of the kits.
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2

Serum and Liver Metabolic Evaluation

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Abdominal aortic blood was centrifuged at 3,000 rpm for 20 min to obtain the serum for further analysis. Liver tissue homogenate was obtained by adding homogenate medium (900 µL) to 100 mg of liver tissue, followed by centrifugation of the mixture at 3,000 rpm for 10 min, and collection of the supernatant for further analysis. The serum levels of total cholesterol (TC), serum triglyceride (TG), serum low-density lipoprotein cholesterol (LDL-C), serum aspartate aminotransferase (AST) (C010-2), serum alanine aminotransferase (ALT) (C009-2) (Jiancheng, Nanjing, China) and liver TG (A0-10017; Dongou, Zhejiang, China) were evaluated according to the manufacturer’s instructions. Hepatic FFA (A042-2-1) and ATP (A095-1-1) (Jiancheng, Nanjing, China) levels were examined using an assay kit according to the manufacturer’s instructions. The content of serum insulin was examined via using a commercial kit (90080; Crystal Chem, Elk Grove Village, IL, USA). HOMA-IR = (fasting blood glucose × fasting insulin content)/22.5 (Haffner et al. 1997 (link)).
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