Recombinant human TGF-β1 was purchased from R&D Systems. SD-208 was obtained from Epichem Pty Ltd (Bentley, WA). Zoledronic acid (Zometa) was obtained from Novartis (Cambridge, MA). Rycal S107 (S107-HCl, FW 245.77) was synthesized as previously described17 (link),37 (link). Anti-TGF-β antibody clone 1D11.16.8 (1D11) and mouse IgG1 isotype control (MOPC-21; unknown specificity) were obtained from BioXCell (West Lebanon, NH). Nox4 inhibitor (GKT137831) was obtained from Ark Pharm, Inc. (Libertyville, IL). Antibodies: Anti-RyR (Affinity Bioreagents, cat. MA3-916, Golden, CO; 1:2,000), anti-Cys NO antibody (Sigma, cat. N0409, St. Louis, MO; 1:2,000), anti-calstabin antibody (Santa Cruz Biotechnology, cat. sc-6173, Santa Cruz, CA; 1:2,500), anti-DNP (Oxyblot, Millipore, Darmstadt, Germany; 1:250), anti-pSMAD3 (Abcam, cat. ab40854, Cambridge, UK; 1:1000), anti-SMAD3 (Abcam, cat. 52903, Cambridge, UK; 1:1000), anti-Nox4 (Abcam, cat. 109225, Cambridge, UK; 1:1000), anti-GAPDH (Sigma, cat. G8795, St. Louis, MO; 1:500) and anti-tubulin (Sigma, cat. 8203, St. Louis, MO; 1:500 each). Fluo-4 AM and Fura-red were obtained from Invitrogen/Molecular probes (cat. F-14201 and cat. F-3020, Eugene, OR).
Anti ryr
Manufactured by Thermo Fisher Scientific
Anti-RyR is a laboratory reagent used for the detection and quantification of ryanodine receptors (RyR) in various biological samples. RyR are integral membrane proteins involved in the regulation of calcium signaling within cells. Anti-RyR is a specific antibody that can be used in techniques such as Western blotting, immunoprecipitation, and immunohistochemistry to study the expression and distribution of RyR.
Automatically generated - may contain errors
Lab products found in correlation
Anti nox4, by Abcam (3 mentions)
Anti cys no antibody, by Merck Group (3 mentions)
Anti calstabin antibody, by Santa Cruz Biotechnology (3 mentions)
Anti smad3, by Abcam (3 mentions)
Anti p smad3, by Abcam (3 mentions)
Zoledronic acid zometa, by Novartis (2 mentions)
Anti tgf β antibody clone 1d11.16.8 1d11, by BioXCell (2 mentions)
Mouse igg1 isotype control mopc 21 unknown specificity, by BioXCell (2 mentions)
Anti dnp, by Merck Group (2 mentions)
Fura red, by Thermo Fisher Scientific (2 mentions)
Recombinant human tgf β1, by R&D Systems (2 mentions)
Anti tubulin, by Merck Group (2 mentions)
Fluo 4 am, by Thermo Fisher Scientific (2 mentions)
Anti gapdh, by Merck Group (2 mentions)
3 protocols using anti ryr
1
Investigating TGF-β1 Signaling Pathways
2
Investigating TGF-β1 Signaling Pathways
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Recombinant human TGF-β1 was purchased from R&D Systems. SD-208 was obtained from Epichem Pty Ltd (Bentley, WA). Zoledronic acid (Zometa) was obtained from Novartis (Cambridge, MA). Rycal S107 (S107-HCl, FW 245.77) was synthesized as previously described17 (link),37 (link). Anti-TGF-β antibody clone 1D11.16.8 (1D11) and mouse IgG1 isotype control (MOPC-21; unknown specificity) were obtained from BioXCell (West Lebanon, NH). Nox4 inhibitor (GKT137831) was obtained from Ark Pharm, Inc. (Libertyville, IL). Antibodies: Anti-RyR (Affinity Bioreagents, cat. MA3-916, Golden, CO; 1:2,000), anti-Cys NO antibody (Sigma, cat. N0409, St. Louis, MO; 1:2,000), anti-calstabin antibody (Santa Cruz Biotechnology, cat. sc-6173, Santa Cruz, CA; 1:2,500), anti-DNP (Oxyblot, Millipore, Darmstadt, Germany; 1:250), anti-pSMAD3 (Abcam, cat. ab40854, Cambridge, UK; 1:1000), anti-SMAD3 (Abcam, cat. 52903, Cambridge, UK; 1:1000), anti-Nox4 (Abcam, cat. 109225, Cambridge, UK; 1:1000), anti-GAPDH (Sigma, cat. G8795, St. Louis, MO; 1:500) and anti-tubulin (Sigma, cat. 8203, St. Louis, MO; 1:500 each). Fluo-4 AM and Fura-red were obtained from Invitrogen/Molecular probes (cat. F-14201 and cat. F-3020, Eugene, OR).
3
Antibody Assay for RyR, Cys-NO, Calstabin, and Oxidative Stress Markers
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Antibodies: Anti-RyR (Affinity Bioreagents, cat. no. MA3-916, Golden, CO, USA; 1:2,000), Anti-RyR1 5029 (custom rabbit polyclonal antibody raised against the C-terminus of human RyR1, Yenzyme, South San Francisco, CA, USA; 1:250 for IP), anti-Cys NO antibody (Sigma, cat. no. N0409, St. Louis, MO, USA; 1:2,000), anti-calstabin antibody (Santa Cruz Biotechnology, cat. no. sc-6173, Santa Cruz, CA, USA, 1:2,500), anti-DNP (Oxyblot, cat. no. S7150, Millipore, Darmstadt, Germany; 1:250), anti-pSMAD3 (Abcam, cat. no. ab40854, Cambridge, UK; 1:1,000), anti-SMAD3 (Abcam, cat. no. 52903, Cambridge, UK; 1:1,1000), and anti-Nox4 (Abcam, cat. no. 109225, Cambridge, UK; 1:1,000).
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