Trichloacetic acid

Manufactured by Merck Group

Sourced in United States

Trichloroacetic acid is a chemical compound used in various laboratory applications. It is a colorless crystalline solid with a pungent odor. Trichloroacetic acid is commonly used as a precipitating agent for proteins and as a reagent in analytical chemistry.

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2 protocols using trichloacetic acid

Diabetic Rat Model Protocol

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Streptozotocin, di-basic sodium phosphate, citric acid, adenosine 5′-triphosphate disodium salt hydrate (ATP), D-mannitol, acarbose and sorbitol were purchased from Sigma Aldrich, Germany. Mono-basic sodium phosphate, sodium hydroxide, sodium bicarbonate, sodium chloride, potassium chloride, calcium chloride di-hydrate, mono-basic potassium phosphate, D r a f t magnesium sulphate, sodium hydrogen carbonate, phenol red, trichloacetic acid (TCA), sodium citrate and sodium potassium tartrate were purchased from Merck, South Africa. Glucose and fructose were purchased from Associated Chemical Enterprise (ACE), South Africa, while metformin and Novo rapid insulin were purchased from a local pharmacy store (Pharmed) in Durban, South Africa.

Chukwuma C.I, & Islam M.S. (2017). Sorbitol increases muscle glucose uptake ex vivo and inhibits intestinal glucose absorption ex vivo and in normal and type 2 diabetic rats. Applied physiology, nutrition, and metabolism = Physiologie appliquee, nutrition et metabolisme, 42(4).

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Antioxidant Activity Assay Protocol

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α,α-Diphenyl-β-picrylhydrazyl free radical (DPPH), linoleic acid, butylated hydroxyanisole (BHA), butylated hydroxytoluene, α-tocopherol, bovine serum albumin, thiobarbituric acid, ferrozine, lecithin, SDS (sodium dodecyl sulfate), ammonium thiocyanate, ferric chloride, KH₂PO₄, and K₂HPO₄ were purchased from Sigma-Aldrich (St. Louis, MO, USA). Sodium dihydrogen phosphate, disodium hydrogen phosphate, NaBr, and trichloacetic acid were obtained from Merck & Co. Inc. (Kenilworth, JN, USA). Tween 20 was obtained from Wako Pure Chemical Industries, Ltd. (Osaka, Japan). HCl, NaCl, and copper sulfate were purchased from the Tokyo Chemical Industry Co. Ltd. (Tokyo, Japan). EDTA was purchased from Mallinckrodt Pharmaceuticals (Raleigh, NC, USA). Ferrous chloride, Coomassie brilliant blue G-250, n-butanol, and phosphotungstic acid were bought from Avantor Performance Materials (Baker analyzed reagents; Center Valley, PA, USA).

Lin Y.P., Lin L.Y., Yeh H.Y., Chuang C.H., Tseng S.W, & Yen Y.H. (2016). Antihyperlipidemic activity of Allium chinense bulbs. Journal of Food and Drug Analysis, 24(3), 516-526.

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