Anti gapdh km9002 antibody

The human prostate cancer cell line PC-3 was cultured in DMEM with 10% fetal bovine serum, penicillin (100 U/ml) and streptomycin (100 ng/ml) at 37°C under 5% CO₂ atmosphere in a humidified incubator. Anti-TLR9 (ab37154) and anti-ODAM (ab169694) antibodies were from Abcam. Anti-GJB1 (GTX11368) and anti-CLDN2 (GTX81901) were from GeneTex. Anti-MMP13 (AB20098a) and anti-ICAM1 (AB21861a) antibodies were from Shanghai Sangon Biotech. Anti-IL8 (sc-7922) antibodies were from Santa Cruz Biotechnology. Anti-FN1 (610077) antibody was from BD Bioscience. Anti-EGR1 (4153) was from Cell Signaling Technologies. Anti-GAPDH (KM9002) antibody was from Tianjin Sungene Biotech.

The cell line Hep-2, normal bronchial epithelium cell line 16HBE and embryonic kidney cell HEK293T were obtained from CCTCC, China Center for Type Culture Collection. The cell line KB-3-1 was provided by Dr. Zhesheng Chen (St. John’s university, USA). The cells were cultured at an moist atmosphere of 37 °C with 5% CO₂ in Dulbecco’s modified Eagle’s medium (DMEM) containing 100 unit/ml penicillin, 10% fetal bovine serum (FBS), and 100 ng/ml streptomycin. Anti-E2F1 (3472) and anti-Survivin (2808) antibodies were obtained from Cell Signaling Technology. Anti-Ki-67 (2724-1) antibody was purchased from Abcam. Anti-Cyclin E (51-1459GR) antibody was from BD Pharmingen. Anti-Flag (F1804) antibody and anti-Flag (A2220) Affinity Gel were from Sigma-Aldrich. Anti-GAPDH (KM9002) antibody was from Tianjin Sungene Biotech. Anti-E2F1 (RLT1442) and anti-α-Tubulin (4777) antibodies were from Ruiying Biological.