Cmir an0001 am03

Human HCC cell lines, SMMC-7721 (the Institute of Biochemistry and Cell Biology, Chinese Academy of Sciences, Shanghai, China) and Huh7 cells (the Liver Cancer Institute, Zhongshan Hospital, Fudan University, Shanghai, China), were cultured in Dulbecco’s modified Eagle medium (DMEM, GIBCO, Carlsbad, CA, USA) supplemented with 10% fetal bovine serum (FBS, GIBCO), 100 U/ml penicillin (GIBCO), and 50 mg/ml streptomycin (GIBCO). HCC cells were maintained in the humidified containing of 5% CO₂ incubator at 37 °C.
The targeted sequences for FOXF2 siRNA (Sense; 5′-GAA AAG AUU UCG UCC UCA Att-3′, Anti-sense; 5′-UUG AGG ACG AAA UCU UUU Ctg-3′) or a scrambled oligonucleotide as a negative control were synthesized by Sangon Biotech (Shanghai) Co., Ltd. (Shanghai, China). MiRNAs vectors, including miR-519a mimics (HmiR0342-MR03), the control vector for miR-519a (CmiR0001-MR03), miR-519a inhibitor (HmiR-AN0588-AM03) and the negative control for the miR-519a inhibitor (CmiR-AN0001-AM03), were obtained from Genecopoeia (Guangzhou, China). Cell transfection was performed using Lipofectamine 2000 (Invitrogen) following the manufacturer’s instructions.