Tissue and cells were collected after washing with cold PBS and then lysed in RIPA lysis buffer (Solarbio) with protease inhibitor cocktail (Roche). Protein concentration was determined with a Lowry protein concentration detection kit (Solarbio). An equal amount of proteins for each sample was subjected to 8–15% sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), and then transferred to nitrocellulose membranes. Antibodies were GATA3 (1:500, Proteintech), COTL1 (1: 200, Proteintech), N-Ras (1:500, Proteintech) and GAPDH (1:1000, Proteintech).
Lowry protein concentration detection kit
Manufactured by Solarbio
The Lowry protein concentration detection kit is a laboratory equipment used to measure the protein concentration in a sample. It is a colorimetric assay that relies on the reaction between copper ions and the peptide bonds in proteins, resulting in a color change that can be measured using a spectrophotometer.
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Lab products found in correlation
Protease inhibitor cocktail, by Roche (2 mentions)
Ripa lysis buffer, by Solarbio (2 mentions)
Cotl1, by Proteintech (1 mentions)
Gata3, by Proteintech (1 mentions)
N ras, by Proteintech (1 mentions)
Gapdh, by Proteintech (1 mentions)
Anti β catenin, by Proteintech (1 mentions)
Anti hnrnp a1, by Santa Cruz Biotechnology (1 mentions)
Anti myh9, by Proteintech (1 mentions)
Anti hif 1α, by Cell Signaling Technology (1 mentions)
Anti β actin, by Proteintech (1 mentions)
2 protocols using lowry protein concentration detection kit
1
Western Blot Analysis of Key Proteins
2
Protein expression analysis of clinical samples
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Clinical samples and cells were collected after washing with cold PBS and then lysed in RIPA lysis buffer (Solarbio) with a protease inhibitor cocktail (Roche). Protein concentration was determined with a lowry protein concentration detection kit (Solarbio). An equal amount of proteins for each sample was subjected to 10% SDS-PAGE and then transferred to nitrocellulose membranes. The primary antibodies include anti-HIF1α (Cell Signaling Technology), anti-MYH9 (Proteintech), anti-β-catenin (Proteintech), anti-hnRNPA1 (Santa Cruz Biotechnology), and anti-β-actin (Proteintech).
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