Alexa fluor 488 coupled goat anti rat secondary antibody

Doxycycline, hydroxypropyl-β-cyclodextrin, poloxamer 407, poloxamer 188, VEGF-C and lipopolysaccharides (LPS) were purchased from Sigma (St. Louis, MO, USA). Antibodies included, anti-LYVE-1, anti-VEGF receptor 3 (VEGFR3) (abcam, Hong Kong, China), anti-Akt, anti-phosphorylated Akt, anti-nuclear factor-kappaB (NF-κB) p65, anti-phosphorylated NF-κBp65, anti-IκB-α, anti-eNOS, anti-phosphorylated eNOS, anti-β-actin, horseradish peroxidase (HRP)-conjugated anti-mouse secondary antibody, HRP-conjugated anti-rabbit secondary antibody, Alexa Fluor 488-coupled goat anti-rat secondary antibody and Alexa Fluor 555-coupled goat anti-rabbit secondary antibody (Cell Signaling Technology, Inc., Danvers, MA).

The antibodies, including anti-lymphatic vessel endothelial hyaluronan receptor-1 (LYVE-1) polyclonal, anti-VEGF-C monoclonal, anti-VEGF receptor 3 (VEGFR3) monoclonal, anti-CD11b monoclonal, anti-F4/80 monoclonal, and Anti- proliferating cell nuclear antigen (PCNA) monoclonal, were from abcam (Hong Kong, China). And the other antibodies, including anti-nuclear factor-kappa B (NF-κB) p65 monoclonal, anti-phosphorylated NF-κB p65 monoclonal, anti-β-actin monoclonal, horseradish peroxidase (HRP)-conjugated anti-mouse secondary antibody, HRP-conjugated anti-rabbit secondary antibody, Alexa Fluor 488-coupled goat anti-rat secondary antibody, Alexa Fluor 555-coupled goat anti-rabbit secondary antibody, were from Cell Signaling Technology Inc. (Danvers, MA). Recombinant HMGB1 (rHMGB1) and Box-A (a specific antagonist of HMGB1) were purchased from IBL International (Hamburg, Germany). VEGF-C was from Sigma (St. Louis, MO, USA).