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2 protocols using gentamycin

1

Antibiotic Resistance Profiling

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Resistance to a set of other agents was assessed with the use of the disc diffusion (DD) method and the E-test method. The former (by Oxoid) was applied for penicillin (P); amikacin (AK); gentamycin (CN); ciprofloxacin (CIP); levofloxacin (LEV); mupirocin (MUP); fusidic acid (FUS); tetracycline (TET), and the latter (by BioMerieux) for ceftaroline (CPT); vancomycin (VA); teicoplanin (TP); linezolid (LZD); daptomycin (DPC); tigecycline (TGC); and spectinomycin (SC), according to the EUCAST guidelines [28 ,51 ].
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2

Carbapenem-resistant bacteria profiling

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Clinical bacteria from human specimens that are resistant to carbapenem antibiotics were collected from a teaching hospital in Indonesia. Bacterial isolates were collected from February to September 2020. The samples were taken every month. According to CLSI (CLSI 2018), carbapenem non-susceptible criteria were minimum inhibitory concentration (MIC) ≥2 mg/L for at least one of imipenem, Ertapenem, and/or Meropenem.
Antimicrobial susceptibility test was performed using semi-automated Vitek 2 (bioMerieux), including Ertapenem (ETP), Meropenem (MEM), Ceftazidime (CAZ), Ceftriaxone (CRO), Cefepime (FEP), Ampicillin-Sulbactam (SAM), Piperacillin-Tazobactam (TZP), Gentamycin (GEN), Amikacin (AMK), Ciprofloxacin (CIP), Ampicillin (AMP), Cefazolin (KZ), Aztreonam (AZT), Trimethoprim-Sulphamethoxazole (SXT) and Tigecycline (TGC). Every species of bacteria was tested for specific antibiotics according to CLSI 2018.
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