Human bronchus and lung samples were obtained from patients undergoing resection for lung tumors and archived formalin-fixed paraffin-embedded material was used. The collection of human respiratory tract tissues was approved by the University of Hong Kong/Hospital Authority Hong Kong West Cluster (HKU/HA HKW IRB) with written informed consent provided by study participants and/or their legal guardians. We used lung tissues from Caucasian (n = 10) and Asian (n = 20) patients. Tissue samples were obtained from formalin-fixed and paraffin-embedded tissues and stained for DBA lectin histochemistry and CT1 immunohistochemistry using methods below.
We used biotinylated SNA, MAA-I, and MAA-II from Vector Laboratories, and a biotinylated DBA from Vector Laboratories (DBA-I). Formalin-fixed and paraffin-embedded tissues were sampled, and slides were incubated with the above lectins at previously documented concentrations. Cad/Sda was detected using a monoclonal antibody provided by K. Klisch (University of Nottingham). Double staining using DBA and influenza nucleoprotein was used for ferret tissues that had been infected with pandemic H1N1 viruses and then euthanized (26 (link)).
We used biotinylated SNA, MAA-I, and MAA-II from Vector Laboratories, and a biotinylated DBA from Vector Laboratories (DBA-I). Formalin-fixed and paraffin-embedded tissues were sampled, and slides were incubated with the above lectins at previously documented concentrations. Cad/Sda was detected using a monoclonal antibody provided by K. Klisch (University of Nottingham). Double staining using DBA and influenza nucleoprotein was used for ferret tissues that had been infected with pandemic H1N1 viruses and then euthanized (26 (link)).