Ultrasoundgate condenser microphone cm16 cmpa

Ultrasonic vocalizations (USVs) were recorded from both male and female pups isolated from their mothers at P4, during the daylight period of the light/dark cycle. Dams and their litters were acclimated for 30 min in the test room. Each pup was removed from the cage containing its mother and littermates and placed in a clean plastic container in a wooden sound-attenuating recording chamber. Each pup was first acclimated in the recording chamber for 30 s then recorded for 10 min. Recordings were acquired using an UltraSoundGate CM16/CMPA condenser microphone (Avisoft Bioacoustics) positioned at a fixed height of 8 cm above the pups, and were amplified and digitized (sampled at 16 bits, 250 kHz) using UltraSoundGate 416 hr 1.1 hardware and Avisoft-RECORDER software (Avisoft Bioacoustics). The data were transferred to Avisoft-SASLab Pro (version 5.2) to analyze spectrograms of vocalizations with settings of 0% overlapping FlatTop windows, 100% frame size, and 256 points fast Fourier transform (FFT) length. The following measures were recorded for each group: number of USV calls, mean duration of USV calls, and mean peak frequency. Recordings were performed with the experimenter blinded to mouse genotypes.

USVs were recorded at P4 for both male and female pups isolated from their mothers during the daylight period of the light/dark cycle. Dams and their litters were acclimated to the test room for 30 min. Each pup was removed from the cage containing its mother and littermates and placed in a clean plastic container in a wooden sound-attenuating recording chamber. Each pup was first acclimated to the recording chamber for 30 s (sec) then recorded for 10 min. Recordings were acquired using an UltraSoundGate CM16/CMPA condenser microphone (Avisoft Bioacoustics) positioned at a fixed height of 8 cm above the pups, and were amplified and digitized (sampled at 16 bits, 250 kHz) using UltraSoundGate 416 hr 1.1 hardware and Avisoft-RECORDER software (Avisoft Bioacoustics). The data were transferred to Avisoft-SASLab Pro (version 5.2) to analyze spectrograms of vocalizations with settings of 0% overlapping FlatTop windows, 100% frame size, and 256 points fast Fourier transform (FFT) length. The following measures were recorded and analyzed for each group: number of USV calls, mean duration of USV calls, mean amplitude, mean peak frequency, maximum peak frequency, mean interval between USV calls, and the latency to call.

Vocalizations were recorded using an UltraSoundGate condenser microphone CM16/CMPA (Avisoft Bioacoustics; Berlin, GER) connected via an UltraSoundGate 416H audio device (Avisoft Bioacoustics) to a personal computer. Acoustic data were recorded as a WAV file with a sampling rate of 250 kHz in 16‐bit format by Avisoft RECORDER USGH (version 4.2.30). Specific recording conditions for each experiment are described below. Avisoft SASLab Pro (version 5.2.15) with a 256 fast Fourier transform was used to generate spectrograms. An investigator blinded to the experimental condition identified calls manually. Calls were categorized as either ultrasonic (>20 kHz) or audible (<20 kHz) mouse squeaks. A separate investigator blinded to the experimental condition listened to the audio files to confirm mouse squeaks. The false‐positive rate for audible mouse squeaks called from the spectrogram was 2.5%.