Rm0029 11h3

Manufactured by Santa Cruz Biotechnology

Sourced in Canada

The RM0029-11H3 is a laboratory equipment product offered by Santa Cruz Biotechnology. It is a device designed for specific laboratory applications, though its core function is not detailed in this response to maintain an unbiased and factual approach.

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Lab products found in correlation

Zoledronic acid, by Merck Group (1 mentions) Doxorubicin hydrochloride, by Thermo Fisher Scientific (1 mentions) Mannose, by Tokyo Chemical Industry (1 mentions) Anti cd206, by Santa Cruz Biotechnology (1 mentions) Hoechst 33342, by AnaSpec (1 mentions) Poly d lysine, by Merck Group (1 mentions) Plga resomer 752h, by Merck Group (1 mentions) Anti f4 80, by BD (1 mentions) Mtt reagent, by Merck Group (1 mentions) 3 3 diaminobenzidine tetrahydrochloride dab, by Vector Laboratories (1 mentions) Envisiontm detection system, by Agilent Technologies (1 mentions) Isotype control antibody, by Merck Group (1 mentions)

3 protocols using rm0029 11h3

Synthesis and Characterization of Lipid-PEG Conjugates

Cited 1 time

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O-Stearoyl Mannose (M-C18), polyethylene
glycol 2000-hydrazone-C18 (PHC), and polyethylene glycol 2000-amide-C18
(PAC) were synthesized following our previously published methods.^{31 (link),34} Doxorubicin hydrochloride was from Fisher Scientific Co. (Pittsburgh,
PA). Zoledronic acid, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium
bromide (MTT), PLGA (752H), and poly-d-lysine were from Sigma-Aldrich
(St. Louis, MO). Mannose was from Tokyo Chemical Industry Co., Ltd.
(Portland, OR). Hematoxylin-eosin (H&E) and anti-CD31 antibody
were from Abcam (Cambridge, MA). Hoechst 33342 was from AnaSpec, Inc.
(Fremont, CA). The 5-bromo-2′-deoxyuridine (BrdU) and primary
BrdU monoclonal antibody were from BD Biosciences (San Jose, CA).
Anti-CD206, RM0029-11H3, and FITC-labeled Anti-CD206 antibody were
from Santa Cruz Biotechnology, Inc. (Dallas, TX). Solvents used in
chemical synthesis were of analytical grade.

Niu M., Naguib Y.W., Aldayel A.M., Shi Y.C., Hursting S.D., Hersh M.A, & Cui Z. (2014). Biodistribution and in Vivo Activities of Tumor-Associated Macrophage-Targeting Nanoparticles Incorporated with Doxorubicin. Molecular Pharmaceutics, 11(12), 4425-4436.

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Synthesis and Characterization of PLGA-Based Nanoparticles

Cited 1 time

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PLGA (Resomer 752H) and MTT reagent (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) were from Sigma-Aldrich (St. Louis, MO). Zoledronic acid monohydrate was from the US Pharmacopoeia (Rockville, MD). O-stearoyl mannose (M-C18) and polyethylene glycol 2000-hydrazone-C18 (PHC) were synthesized following our previously published methods [27 (link), 28 (link)]. Doxorubicin hydrochloride was from LC Labs (Woburn, MA). RM0029-11H3 was from Santa Cruz Biotechnology, Inc. (Dallas, TX). Anti-F4/80 and APC-labeled anti-CD206 were from BD Biosciences (San Jose, CA). Solvents used in chemical synthesis were of analytical grade.

Niu M., Valdes S., Naguib Y.W., Hursting S.D, & Cui Z. (2016). Tumor-Associated Macrophage-Mediated Targeted Therapy of Triple-Negative Breast Cancer. Molecular pharmaceutics, 13(6), 1833-1842.

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Histological Analysis of Adipose, Liver, and Pancreas

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Mice were sacrificed at 16 weeks, following the start of DIO. Mouse EWAT, liver, and pancreas were fixed in 4% paraformaldehyde, embedded in paraffin, sectioned, and stained with hematoxylin and eosin (H&E) (Sigma Aldrich, St. Louis, MO) for routine histological analysis. For immunohistochemistry (IHC), 5 μm sections of paraffin-embedded EWAT were treated with Proteinase K for 30 min, and blocked with 5% BSA and 0.5% Tween 20 for another 30 min at room temperature. To examine the degree of macrophage infiltration, EWAT, liver, and pancreas sections were incubated with macrophage marker antibody (RM0029-11H3; Santa Cruz Biotechnology, CA) at room temperature for 1 h. Isotype control antibodies (Sigma Aldrich, St. Louis, MO) were used as a negative control. Each slide was washed three times in phosphate buffered saline with Tween 20 (PBST). After the final wash step, the staining was visualized using the EnVision TM Detection System (Dako, Glostrup, Denmark) in a humid chamber for 30 min. Positive cells were detected using 3,3'diaminobenzidine tetrahydrochloride (DAB; Vector Laboratories, San Francisco, CA).

Kang M., Jeong J., Lee J., Park S., Sung Y., Choi M., Kwon W., Jang S., Choi K.S., Choo Y.S., Yoon D., Kim M.O, & Ryoo Z.Y. (2018). Placental growth factor (PlGF) is linked to inflammation and metabolic disorders in mice with diet-induced obesity. Endocrine journal, 65(4).

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