UNC0638 and BIX01294 were purchased from Sigma (St. Louis, MO, USA), BI2536 was purchased from Selleck (Shanghai, China), BRD9539 and A-366 were purchased from MedChem Express (Shanghai, China). RIPA were purchased from Beyotime (Nantong, China). The study included the following primary antibodies: anti-G9a antibody (Cell Signaling Technology, Danvers, MA, USA), anti-Plk1 antibody (Cell Signaling Technology), rabbit anti-p53 antibody (Abcam, Cambridge, MA, USA), mouse anti-p53 antibody (Santa Cruz Biotechnology, Dallas, USA), anti-pan-methyl lysine antibody (Abcam), anti-histone H3 antibody (Santa Cruz Biotechnology), anti-histone H3 dimethyl (K9) antibody (Cell Signaling Technology), anti-β-actin antibody (Cell Signaling Technology), anti-GAPDH antibody (Cell Signaling Technology), and anti-GFP antibody (Santa Cruz Biotechnology). Horseradish peroxidase (HRP)-conjugated anti-mouse and anti-rabbit secondary antibodies were purchased from KangChen Bio-Tech (Shanghai, China), and HRP-conjugated donkey anti-goat IgG was purchased from Santa Cruz Biotechnology. Protein A/G agarose beads were purchased from Santa Cruz Biotechnology, and protease inhibitor cocktail was purchased from Roche Applied Science (Basel, Switzerland).
Anti histone h3 antibody
Manufactured by Santa Cruz Biotechnology
Sourced in United States
The Anti-histone H3 antibody is a laboratory reagent used in various research applications. It is a specific antibody that recognizes and binds to the histone H3 protein, a key component of the nucleosome, which is the fundamental unit of chromatin in eukaryotic cells. This antibody can be utilized in techniques such as Western blotting, immunoprecipitation, and chromatin immunoprecipitation to study the localization, interactions, and post-translational modifications of histone H3.
Automatically generated - may contain errors
Lab products found in correlation
Sytox green, by Thermo Fisher Scientific (3 mentions)
Anti gfp antibody, by Santa Cruz Biotechnology (1 mentions)
Anti g9a antibody, by Cell Signaling Technology (1 mentions)
Unc0638, by Merck Group (1 mentions)
Bix 01294, by Merck Group (1 mentions)
Hrp conjugated donkey anti goat igg, by Santa Cruz Biotechnology (1 mentions)
Rabbit anti p53 antibody, by Abcam (1 mentions)
Mouse anti p53 antibody, by Santa Cruz Biotechnology (1 mentions)
Anti plk1 antibody, by Cell Signaling Technology (1 mentions)
Anti β actin antibody, by Cell Signaling Technology (1 mentions)
Anti gapdh antibody, by Cell Signaling Technology (1 mentions)
Bi2536, by Selleck Chemicals (1 mentions)
Protease inhibitor cocktail, by Roche (1 mentions)
Protein a g agarose bead, by Santa Cruz Biotechnology (1 mentions)
Radioimmunoprecipitation assay (ripa), by Beyotime (1 mentions)
Skim milk, by Nacalai Tesque (1 mentions)
Anti mcm2 antibody d7g11, by Cell Signaling Technology (1 mentions)
Anti gapdh antibody 14c10, by Cell Signaling Technology (1 mentions)
Anti cdc6 antibody sc 9964, by Santa Cruz Biotechnology (1 mentions)
Anti cdt1 d10f11, by Cell Signaling Technology (1 mentions)
3 protocols using anti histone h3 antibody
1
Chromatin-Modifying Enzyme Inhibitor Protocol
2
Western Blot Analysis of Cell Cycle Proteins
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Proteins in 2 × SDS loading buffer were separated on a 10% SDS polyacrylamide gel and transferred to Immobilon-P PVDF membranes (Merck) using tank blotting (Bio-Rad), with the exception of histone H3 and GAPDH, which were separated on a 15% SDS polyacrylamide gel and transferred using a semi-dry blotting system (ATTO). After blocking with 5% skim milk (Nacalai Tesque), the membranes were incubated with the following primary antibodies: anti-Mcm2 antibody (D7G11; Cell Signaling Technology), anti-GAPDH antibody (14C10; Cell Signaling Technology), anti-histone H3 antibody (1G1, (41 (link))), anti-Cdc6 antibody (sc-9964; Santa Cruz Biotechnology), anti-Cdt1 (D10F11; Cell Signaling Technology), anti-Orc2 antibody (3G6; Santa Cruz Biotechnology), and anti-Cdc45 antibody (D7G6; Cell Signaling Technology). After incubation with peroxidase-conjugated secondary antibodies (GE Healthcare), the signals were detected using ImmunoStar LD (Fujifilm).
3
Immune Cell Isolation and Functional Assays
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Percoll was purchased from GE Healthcare (Little Chalfont, UK). Phorbol 12-myristate 13-acetate (PMA), DNase I, Sodium hydroxide (NaOH), cytochalasin D, ASA, DXM, Calcein AM, and dichlorofluorescein diacetate (DCF-DA) were purchased from Sigma-Aldrich (St. Louis, MO, USA). Anti-histone H3 antibody, anti-phosphorylated nuclear factor κB (anti-p-NF-κB, p65) antibody, horseradish peroxidase (HRP) secondary antibody, and secondary antibodies coupled to AF488 or AF555 were purchased from Santa Cruz Biotechnology (CA, USA). Anti-myeloperoxidase (MPO) antibody and anti-β actin antibody were purchased from Abcam(Cambridge, UK). SYTOX Green, Quant-iTPicoGreen double-stranded deoxyribonucleic acid assay kits were purchased from ThermoFisher Scientific (Basingstoke, UK). Cell proliferation reagent WST-1 was purchased from Roche (Basal, Switzerland).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!