Six to eight week-old non-obese diabetic (NOD) severe combined immunodeficiency (SCID) NOD.CB17-Prkdcscid/NCrHsd mice (Envigo, Huntingdon, UK) were subcutaneously injected with a mixture containing growth factors reduced Matrigel and heparin (50 U/mL) supplemented with HGF and VEGF (200 ng/mL for each growth factor) or with RPMI8266 cell (5x105 cells/mL) CM. Assuming an effect-size of 0.4 with statistical significance of α < 0.05 and a power of 80% we used n. 6 mice for each group for a total of 64 mice. This number was increased to 72 taking into account an expected drop-out rate of 10% for the treatment group. Drop-outs, specifically, may be caused by failure of plug injection. Mice were administered intraperitoneally every 72 hours with MP0250 (4 mg/Kg) or with PBS. After 14 days, mice were sacrificed, and the Matrigel plugs removed, weighed, and homogenized in cold PBS. The hemoglobin content of the plugs was calculated using Drabkin’s reagent kit 525 (Sigma-Aldrich) and normalized to the weights. Mice were housed according to the Institutional Animal Care and Use Committee of the University of Bari Medical School, Bari, Italy (licence n. 548/2016PR).
Cb17 prkdcscid ncrhsd mice
Manufactured by Inotiv
Sourced in United Kingdom
The CB17-Prkdcscid/NCrHsd mice are a strain of immunodeficient mice commonly used in research. They lack functional T and B cells, making them useful for studies involving xenografts and other applications where an intact immune system is not desired.
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2 protocols using cb17 prkdcscid ncrhsd mice
1
Angiogenic Potential of RPMI8266 Cell Secretome
2
Modeling Multiple Myeloma Tumors in Mice
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Twenty female 8- to 10-week-old NOD.CB17- Prkdcscid/NCrHsd mice (NOD-SCID; Envigo, Huntingdon, UK) were injected intratibially with 2×105 RPMI-8226 cells suspended in phosphate-buffered saline. Mice were treated with the anti-JAM-A monoclonal antibody (Sigma-Aldrich, St. Louis, MO, USA, mouse monoclonal clone J10.4) recognizing the distal membrane extracellular domain of JAM-A.
Twenty female 6- to 8-week-old NOD-SCID mice were injected subcutaneously (s.c.), into the right flank, with 1×107 RPMI-8226 cells suspended in 200 mL RPMI-1640 medium and 200 mL MatrigelTM as described previously16 (link) and detailed in the Online Supplementary Methods.
Twenty female 6- to 8-week-old NOD-SCID mice were injected subcutaneously (s.c.), into the right flank, with 1×107 RPMI-8226 cells suspended in 200 mL RPMI-1640 medium and 200 mL MatrigelTM as described previously16 (link) and detailed in the Online Supplementary Methods.
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