D luciferin potassium salt

Subcutaneous tumor-bearing nude mice were intraperitoneally injected with d-luciferin potassium salt (S31462, Shanghai Yuanye Bio-Technology Co., Ltd) and imaged by In-Vivo Imaging System (QuickView 3000, LABATECH GmbH. Bergstrasse 14 A-5020, Salzburg, Austria) which was performed 15 min after injection. In vivo imaging was performed before treatments and once again two weeks after treatments. The photon signaling intensity of each time point was recorded in each treatment group.

DHT (purity ≥ 98.0%), Tan I (purity ≥ 98.0%), CT (purity ≥ 98.0%), Tan IIA (purity ≥ 98.0%), and D-luciferin potassium salt (purity ≥ 98.0%) were purchased from Yuanye Biotechnology (Shanghai, China). PMA, DDP, PTX, Dulbecco′s modified Eagle′s Medium (DMEM), RPMI-1640, 0.25% trypsin-EDTA, fetal bovine serum (FBS), and penicillin/streptomycin solution were purchased from Gibco (Grand Island 14072, NY, USA). Antibodies against β-actin, and goat anti-rabbit were purchased from Proteintech (Wuhan, China). Histone H3 (citrulline R2 + R8 + R17), Mpo, and Ly6G were purchased from Abcam (Cambridge, UK). Matrigel was purchased from Thermo Fisher Scientific (Waltham 02454, MA, USA). The dsDNA HS Assay Kit for Qubit was purchased from Yeasen (Shanghai, China). Crystal violet and ROS assay kits were purchased from Solarbio (Beijing, China).