Rabbit monoclonal anti pakt s473 antibody

Manufactured by Cell Signaling Technology

Rabbit monoclonal anti-pAKT S473 antibody is a laboratory reagent used to detect and quantify the phosphorylated form of the serine 473 residue of the AKT protein. This antibody is produced in rabbits and is specific for the phosphorylated form of AKT.

Automatically generated - may contain errors

Lab products found in correlation

Rabbit monoclonal anti akt antibody, by Cell Signaling Technology (2 mentions) Mouse monoclonal anti β tubulin antibody, by Merck Group (2 mentions) Eclipse ti confocal laser scanning microscope, by Nikon (1 mentions) Rabbit monoclonal anti notch3 antibody, by Cell Signaling Technology (1 mentions) Rabbit monoclonal anti pten antibody, by Cell Signaling Technology (1 mentions) Mouse monoclonal anti flag m2 antibody, by Merck Group (1 mentions)

Close spelling variants of this product

Anti-pAKT S473 Monoclonal rabbit anti-pAKT Rabbit anti-pAkt antibody PAKT S473 Monoclonal rabbit antibody Monoclonal rabbit

2 protocols using rabbit monoclonal anti pakt s473 antibody

Immunofluorescence and Western Blot Analysis of AKT Signaling

Cited 1 time

Check if the same lab product or an alternative is used in the 5 most similar protocols

IFA and Western blotting were performed as previously described (80 (link)). For Western blotting, rabbit monoclonal anti-AKT antibody (Cell Signaling Technology, Danvers, MA), rabbit monoclonal anti-pAKT T308 antibody (Cell Signaling Technology), rabbit monoclonal anti-pAKT S473 antibody (Cell Signaling Technology), and mouse monoclonal anti-β-tubulin antibody (Sigma, St. Louis, MO) were used as primary antibodies. A rat monoclonal antibody to LANA (Abcam, Cambridge, MA) and a mouse monoclonal antibody to ORF65 (81 (link)) were used for IFA. Images were obtained using a Nikon Eclipse E400 fluorescence microscope equipped with a digital camera or a Nikon Eclipse Ti confocal laser scanning microscope (Nikon Instruments Inc., Melville, NY). Nikon NIS Elements F or Ar microscope imaging software was used for image analysis.

Lee M.S., Yuan H., Jeon H., Zhu Y., Yoo S., Shi S., Krueger B., Renne R., Lu C., Jung J.U, & Gao S.J. (2016). Human Mesenchymal Stem Cells of Diverse Origins Support Persistent Infection with Kaposi’s Sarcoma-Associated Herpesvirus and Manifest Distinct Angiogenic, Invasive, and Transforming Phenotypes. mBio, 7(1), e02109-15.

+ Open protocol

+ Expand

Molecular Expression Vectors and Antibody Validation

Check if the same lab product or an alternative is used in the 5 most similar protocols

The E. coli expression vectors, pET28GST-LIC, pET28a-MHL and pET32a-LIC were constructed in our laboratory. All the primers used in this study were provided in Supplementary Table S1.
Antibodies used in this study are as follows: mouse monoclonal anti-β-tubulin antibody (Sigma-Aldrich, Cat# T4026, 1:5000), mouse monoclonal anti-FLAG M2 antibody (Sigma-Aldrich, Cat# A8592, 1:5000), rabbit monoclonal anti-HA antibody (Sigma-Aldrich, Cat# H6908, 1:1000), rabbit monoclonal anti-NOTCH3 antibody (Cell Signaling Technology, Cat# 5276, 1:1000), rabbit monoclonal anti-H3K4me3 antibody (Cell Signaling Technology, Cat# 9751, 1:1000), rabbit monoclonal anti-PTEN antibody (Cell Signaling Technology, Cat# 9559, 1:1000), rabbit monoclonal anti-AKT antibody (Cell Signaling Technology, Cat# 4691, 1:1000), rabbit monoclonal anti-p- AKT (S473) antibody (Cell Signaling Technology, Cat# 4060, 1:1000), rabbit monoclonal anti-SLC12A5 antibody (Proteintech, Cat# 28724-1-AP, 1:1000), rabbit monoclonal anti-TCF19 antibody (Affinity Bioscience, Cat# DF9971, 1:1000).

Huang X., Zhang C., Shang X., Chen Y., Xiao Q., Wei Z., Wang G., Zhen X., Xu G., Min J., Shen S, & Liu Y. (2024). The NTE domain of PTENα/β promotes cancer progression by interacting with WDR5 via its SSSRRSS motif. Cell Death & Disease, 15(5), 335.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!