Western blots were performed as previously described[11 (link)]. The following reagents were used: rabbit polyclonal antibody to cyclooxygenase-2 (COX-2) (AbcamInc, Cambridge, MA, United States), glutamate-cysteine ligase modifier subunit (GCLM) (AbcamInc, Cambridge, MA, United States), inducible nitric oxide (NO) synthase (iNOS) (Santa Cruz Biotechnology, Santa Cruz, CA, United States), endothelial NO synthase (eNOS) (Santa Cruz Biotechnology, Santa Cruz, CA, United States), glutamate-cysteine ligase catalytic subunit (GCLC) (AbcamInc, Cambridge, MA), glutathione (GSH) peroxidase (GPx) (AbcamInc, Cambridge, MA, United States), heme oxygenase-1 (HO-1) (AbcamInc, Cambridge, MA, United States), superoxide dismutase (SOD) (Santa Cruz Biotechnology, Santa Cruz, CA, United States), glyceraldehyde-3-phosphate dehydrogenase (GADPH) (Cell Signaling, Danvers, MA, United States), rabbit monoclonal antibody to NF-κB (Cell Signaling, Danvers, MA, United States), catalase (CAT) (Rockland Immunochemicals, Limerick, PA, United States), and mouse monoclonal antibody to nicotinamide adenine dinucleotide phosphate (NAD(P)H) quinone oxidoreductase-1 (NQO-1) (AbcamInc, Cambridge, MA, United States) followed by secondary anti-rabbit or mouse immunoglobulin G (Cell Signaling, Danvers, MA, United States).
Superoxide dismutase sod
Manufactured by Santa Cruz Biotechnology
Sourced in United States
Superoxide dismutase (SOD) is an enzyme that catalyzes the conversion of superoxide radicals into oxygen and hydrogen peroxide. It plays a crucial role in the defense against oxidative stress within cells.
Automatically generated - may contain errors
Lab products found in correlation
Cox 2, by Abcam (1 mentions)
Glyceraldehyde 3 phosphate dehydrogenase gadph, by Cell Signaling Technology (1 mentions)
Nad p h quinone oxidoreductase 1 nqo 1, by Abcam (1 mentions)
Heme oxygenase 1 ho 1, by Abcam (1 mentions)
Ripa buffer, by Thermo Fisher Scientific (1 mentions)
Ecl western blotting detection reagent, by Thermo Fisher Scientific (1 mentions)
Bcl2 associated x (bax), by Cell Signaling Technology (1 mentions)
Streptomycin, by Welgene (1 mentions)
Catalase cat, by Santa Cruz Biotechnology (1 mentions)
Caspase 3, by Cell Signaling Technology (1 mentions)
3 4 5 dimethylthiazol 2 yl 2 5 diphenyltetrazolium bromide mtt, by Avantor (1 mentions)
β actin, by Santa Cruz Biotechnology (1 mentions)
Dulbecco s modified eagle medium dmem, by Welgene (1 mentions)
Bcl 2, by Santa Cruz Biotechnology (1 mentions)
Penicillin, by Welgene (1 mentions)
2 protocols using superoxide dismutase sod
1
Western Blot Analysis of Oxidative Stress Markers
2
Antioxidant Effects of Modulators
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Dulbecco’s Modified Eagle Medium (DMEM), fetal bovine serum (FBS), penicillin, and streptomycin were purchased from Welgene Inc. (Korea). 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazoliumbromide (MTT) was purchased from Amresco Inc. (Solon, OH, USA). The primary antibodies of Nrf2, HO-1, β-actin, Bcl-2, superoxide dismutase (SOD) and catalase (CAT) were purchased from Santa Cruz Biotechnology Inc. (Santa Cruz, CA, USA), Bax and Caspase-3 were purchased from Cell Signaling Technology (Danvers, MA, USA). Hydrogen peroxide solution (H2O2), Protoporphyrin IX (SnPP), Cobalt protoporphyrin (CoPP) and DCF-DA (2′, 7′-dichloroflourescin diacetate) were bought from Sigma Aldrich (St. Louis, MO, USA). RIPA buffer and ECL Western blotting detection reagents were purchased from Fisher Scientific Inc. (Waltham, MA, USA).
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