Recombinant human adiponectin

Rabbit polyclonal antibody specific for phosphate-p85, a heterodimer of phosphatidylinositol 3 kinase (PI3K), p-Akt, p-IKK were purchased from Cell Signaling Technology (Danvers, MA, USA). Rabbit polyclonal antibodies specific for PI3K, Akt, IKK, NF-κB, β-actin, and mouse polyclonal antibodies specific for OSM were purchased from Santa Cruz Biotechnology (Santa Cruz, CA, USA). The recombinant human adiponectin was purchased from PeproTech (Rocky Hill, NJ, USA). PI3K inhibitors (Wortmannin and Ly294002), Akt inhibitor, and NF-κB inhibitors pyrrolidine dithiocarbamate (PDTC) and L-1-tosylamido-2-phenylenylethyl chloromethyl ketone (TPCK) were purchased from Sigma-Aldrich (St. Louis, MO, USA). Small-interfering RNAs (siRNAs) against p85, Akt, and p65 were purchased from Dharmacon Research (Lafayette, CO, USA). OSM ELISA kit was purchased from R&D Systems (Minneapolis, MN, USA). DMEM, fetal bovine serum (FBS), and all the other cell culture reagents were purchased from Gibco life technologies (Grand Island, NY, USA).

A mouse monoclonal anti-cytokeratin 7 antibody, rabbit polyclonal anti-actin antibody and JAK2/STAT inhibitor (AG490) were obtained from Sigma-Aldrich (St. Louis, Mo., USA). Bromodeoxyuridine (BrdU) was obtained from Thermo Fisher Scientific (Waltham, MA). An anti-BrdU antibody was obtained from Dako (Tokyo, Japan). A rabbit polyclonal anti-vimentin (H-84) antibody was obtained from Santa Cruz Biotechnology (Santa Cruz, CA, USA). A PI3K inhibitor (LY294002) and mitogen-activated protein kinase (MAPK) inhibitor (U0126) were purchased from Calbiochem-Novabiochem Corporation (San Diego, CA). A mouse monoclonal anti-β-catenin antibody was obtained from BD Transduction Laboratories (San Jose, CA). Rabbit polyclonal anti-lipolysis-stimulated receptor (LSR) and anti-tricellulin (TRIC) antibodies were obtained from Zymed Laboratories (San Francisco, CA). Alexa 488 (green)-conjugated anti-rabbit IgG and Alexa 594 (red)-conjugated anti-mouse IgG antibodies were purchased from Molecular Probes, Inc. (Eugene, OR). Recombinant human leptin and recombinant human adiponectin were obtained from PeproTech (Rocky Hill, NJ). Metformin was purchased from Wako (Tokyo, Japan). Berberine was obtained from Tokyo Chemical Industry, Inc. (Tokyo, Japan).