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Alexa fluor 488 goat anti rat antibody

Manufactured by Thermo Fisher Scientific
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The Alexa Fluor 488 goat anti-rat antibody is a secondary antibody that is conjugated with the Alexa Fluor 488 fluorescent dye. This antibody is designed to specifically bind to primary antibodies raised in rat, and the Alexa Fluor 488 label allows for the detection and visualization of target proteins or cells using fluorescence-based techniques.

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Lab products found in correlation

Zoe fluorescent cell imager, by Bio-Rad (2 mentions) Goat serum, by Merck Group (2 mentions) Bx51 microscope, by Olympus (2 mentions) Xm10 camera, by Olympus (2 mentions) Rat anti mouse cd31 antibody, by BD (1 mentions) Clsm 710, by Zeiss (1 mentions) Vectashield hardset mounting medium with dapi, by Vector Laboratories (1 mentions) Fv1000 confocal microscope, by Olympus (1 mentions) Anti ssea3 antibody, by Abcam (1 mentions) Tnb blocking buffer, by PerkinElmer (1 mentions) Fjk 16, by Thermo Fisher Scientific (1 mentions) Cyanine 3, by PerkinElmer (1 mentions) Anti epcam, by Thermo Fisher Scientific (1 mentions) M1 70, by Thermo Fisher Scientific (1 mentions) Mounting media, by Vector Laboratories (1 mentions) Methanol, by Merck Group (1 mentions) S3023, by Agilent Technologies (1 mentions) Axiovision fluorescent camera, by Zeiss (1 mentions) Axiovision software, by Zeiss (1 mentions) Triton x 100, by Merck Group (1 mentions)

Close spelling variants of this product

Alexa Fluor 488 Goat anti-Rat IgG secondary antibody Alexa Fluor 488-conjugated goat anti-rat IgG antibody Alexa Fluor 488-conjugated goat anti-rat antibody Alexa Fluor 488 goat anti-rat secondary antibody Alexa Fluor 488-labeled goat anti-rat secondary antibody Alexa Fluor®488-conjugated Goat Anti-Rat secondary antibody Alexa Fluor 488-conjugated polyclonal goat anti-rat antibody Alexa Fluor® 488 goat anti–rat IgG antibody Alexa Fluor 488 goat anti‐rat IgG (H+L) antibody Alexa Fluor 488 goat anti-rat

8 protocols using alexa fluor 488 goat anti rat antibody

1

Immunofluorescence Staining of Mouse CD31

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IF methods were described previously (8 (link)). Antibodies used were: 1:100 rat anti-mouse CD31 antibody (BD, 550274), 1:200 Alexa Fluor® 488 goat anti-rat antibody (Invitrogen, A11006), and 1:500 monoclonal mouse anti-α-smooth muscle actin (SMA) Cy3 antibody (Sigma, C6198). Slides were mounted with Vectashield Hardset Mounting Medium with DAPI (Vector Labs) and imaged on a Zeiss CLSM 710 or 700 Spectral Confocal Laser Scanning Microscope.
Xiao L., Kim D.J., Davis C.L., McCann J.V., Dunleavey J.M., Vanderlinden A., Xu N., Pattenden S.G., Frye S.V., Xu X., Onaitis M., Monaghan-Benson E., Burridge K, & Dudley A.C. (2015). Tumor endothelial cells with distinct patterns of TGFβ-driven endothelial-to-mesenchymal transition. Cancer research, 75(7), 1244-1254.
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2

Immunocytochemical Analysis of Stem Cells

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The cells were fixed with paraformaldehyde solution in PBS (4% w/v), permeabilized with Triton X-100 (0.1%), treated with bovine serum albumin (Affimetrix, USA) in PBS (3%) for 1 h to block the nonspecific binding, washed in PBS thrice for 15 min, incubated with Anti-SSEA3 antibody (Abcam, UK) and Anti-CD105 antibody (BD, USA) overnight at 4 °C, washed with PBS, exposed to Alexa Fluor 488 goat anti-rat antibody (Invitrogen Life Technologies, USA) for 1 h at 37 °C, and counterstained with 4',6-diamidino-2-phenylindole (DAPI). Images of the stained cells were obtained using an FV1000 Olympus confocal microscope (Olympus, Japan).
Kim H., Park K., Yon J.M., Kim S.W., Lee S.Y., Jeong I., Jang J., Lee S, & Cho D.W. (2022). Predicting multipotency of human adult stem cells derived from various donors through deep learning. Scientific Reports, 12, 21614.
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3

Quantifying Immune Populations in Tumor Sections

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For analysis of CD3 (AbD Serotec, KT3, 1:100), CD4 (BioXCell, GK1.5, 15 μg/mL), CD8 (BioXcell, 2.43, 15 μg/mL), and Foxp3 (EBioscience, FJK-16s, 1:40), frozen sections fixed in 100% methanol were analyzed as previously described 11 (link). For analysis of FAP (R&D Systems, BAF3715; 2 mg/mL), frozen sections fixed in 3% formaldehyde were incubated with TNB blocking buffer (Perkin Elmer) and anti-FAP for 18 hours at 4C followed by detection using tyramide signal amplification system with Cyanine 3 (Perkin Elmer); then incubated with anti-EpCAM (eBioscience; 14-5791-85; 1:100) for 1 hour at room temperature followed by detection using Alexa Fluor 488 goat anti-rat antibody (Invitrogen; A11006; 1:500). For quantification, the number of cells was counted per 40× field with a minimum of 4 fields per tumor quantified.
Beatty G.L., Winograd R., Evans R.A., Long K.B., Luque S.L., Lee J.W., Clendenin C., Gladney W.L., Knoblock D.M., Guirnalda P.D, & Vonderheide R.H. (2015). Exclusion of T Cells From Pancreatic Carcinomas in Mice is Regulated by Ly6Clow F4/80+ Extra-tumor Macrophages. Gastroenterology, 149(1), 201-210.
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4

TUNEL and CD11b+ Cell Quantification

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The In Situ Cell Death Detection Kit, TMR red (Roche, 12156792910) was used, following the manufacturer’s instructions on muscle and skin tissue cryosections. To detect CD11b+ cells, sections were incubated overnight at 4 °C with rat anti-mouse CD11b (5 μg ml−1, M1/70, Thermo Fisher Scientific, 14-0112-82) in staining buffer. After PBS-T washes, sections were incubated with Alexa Fluor 488 goat anti-rat antibody (2.67 µg ml−1, Thermo Fisher Scientific, A48262TR), washed with PBS-T, and counterstained with DAPI (1 μg ml−1) before mounting with Fluoroshield. Two tissue section levels were evaluated per sample to determine the percentage of TUNEL+ apoptotic cells over total CD11b+ cells, examining three fields per section within the injury site.
Lu Y.Z., Nayer B., Singh S.K., Alshoubaki Y.K., Yuan E., Park A.J., Maruyama K., Akira S, & Martino M.M. (2024). CGRP sensory neurons promote tissue healing via neutrophils and macrophages. Nature, 628(8008), 604-611.
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5

Immunocytochemistry of Mouse Embryonic Cells

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OSE, OSE-SC, OSE-NS, MEFs from FVB/N mice and OSN2 cells were grown on gelatin-coated glass coverslips for 24 hours and then fixed using methanol (Sigma-Aldrich; Cat#: 322415-1L). Fixed cells were washed with PBS, and then blocked using goat serum (Sigma-Aldrich; Cat#: NS02L). Primary Rat anti CK8 (TROMA1) antibody (University of Iowa Developmental Hybridoma Bank; Cat#: AB_531826) was added to coverslips overnight at 4°C, followed by PBS washes. Alexa Fluor 488 goat anti rat antibody (Thermo Fisher; Cat#: A-11006) was added for one hour using the manufacturer-recommended concentration. One drop of mounting media (Vector Laboratories; Cat#: H-1000) was used to adhere coverslips to slides, and cells were imaged using an Olympus BX51 microscope and Olympus XM10 camera at 10x magnification. GFP expression conveyed by FUGW viral transductions was detected using a BioRad ZOE Fluorescent Cell Imager at 20x.
Yamulla R.J., Nalubola S., Flesken-Nikitin A., Nikitin A.Y, & Schimenti J.C. (2020). Most Commonly Mutated Genes in High-Grade Serous Ovarian Carcinoma Are Nonessential for Ovarian Surface Epithelial Stem Cell Transformation. Cell reports, 32(9), 108086.
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6

Immunofluorescence Staining of Mouse Cells

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OSE, OSE-SC, OSE-NS, MEFs from FVB/N mice and OSN2 cells were grown on gelatin-coated glass cover slips for 24 hours and then fixed using methanol. Fixed cells were washed with PBS, and then blocked using goat serum (Sigma Aldrich; Cat#: NS02L). Primary Rat anti CK8 (TROMA1) antibody (University of Iowa Developmental Hybridoma Bank; Cat#: AB_531826) was added to coverslips overnight at 4˚C, followed by PBS washes. Alexa Fluor 488 goat anti rat antibody (ThermoFisher; Cat#: A-11006) was added for one hour using the manufacturer-recommended concentration. One drop of mounting media (Vector Laboratories; Cat#: H-1000) was used to adhere cover slips to slides, and cells were imaged using an Olympus BX51 microscope and Olympus XM10 camera at 10x magnification. GFP expression conveyed by FUGW viral transductions was detected using a BioRad ZOE Fluorescent Cell Imager at 20x.
Yamulla R.J., Nalubola S., Flesken‐Nikitin A., Nikitin A.Y., & Schimenti J.C. (2020). Most commonly mutated genes in High Grade Serous Ovarian Carcinoma are nonessential for ovarian surface epithelial stem cell transformation.
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7

Immunofluorescence Imaging of Cellular Structures

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For immunofluorescence, the cells were treated with Cy3- or FITC-conjugated 2OMePS oligonucleotides for 4 h, washed three times with PBS containing Ca2+ and Mg2+ solution, and fixed with methanol at −20°C for 10 min. Then, the cells were washed and stored in PBS at 4°C for future immunofluorescence analysis. For colocalisation, the cells were treated with 0.1% Triton-X100 (Sigma-Aldrich, München, Germany) in PBS for 10′, washed three times with PBS plus, and then blocked with PBS containing 1% bovine serum albumin (BSA, Sigma-Aldrich, St. Louis, MO, USA) for 1 h. After this, the cells were incubated with rat anti-mouse NCL antibody (1:200 dilution, Bio-Rad, Hercules, CA, USA), washed three times with PBS plus, and treated with 1:500 Alexa Fluor 488 goat anti-rat antibody (Life Technologies, Carlsbad, CA, USA) for 1 h. 4′,6-diamidino-2-phenylindole (DAPI) (1:5000 dilution, Sigma-Aldrich, St. Louis, MO, USA) staining, which was then performed for 2′, after which the cells were washed and mounted with the fluorescent mounting medium S3023 (Dako, Tokyo, Japan) onto glass slides. The visualization was carried out on a Leica fluorescent microscope, and the pictures were taken by an Axiovision fluorescent camera and the Axiovision software (Zeiss, Oberkochen, Germany).
Aoki Y., Rocha C.S., Lehto T., Miyatake S., Johansson H., Hashimoto Y., Nordin J.Z., Mager I., Aoki M., Graham M., Sathyaprakash C., Roberts T.C., Wood M.J., Behlke M.A, & Andaloussi S.E. (2021). Fine Tuning of Phosphorothioate Inclusion in 2′-O-Methyl Oligonucleotides Contributes to Specific Cell Targeting for Splice-Switching Modulation. Frontiers in Physiology, 12, 689179.
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8

Labeling Antibodies with Fluorescent Dyes

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2-distearoyl-sn-glycero-3-phospho-ethanolamine-N-[maleimide (polyethylene glycol)-3400] (DSPE-PEG3400-Malemide) was purchased from Laysan Bio, Inc. (Arab, AL, USA) and stored in chloroform as 3.4 mM solution. Traut’s reagent (2-iminothiolane) was purchased from Thermo Fisher Scientific (Rockford, IL, USA). The reagent was dissolved in double-distilled water at 5 mg/ml and stored in aliquots at −20° C. AffiniPure Fc fragment specific IgG was purchased from Jackson ImmunoResearch (West Grove, PA). Mouse anti-human and rat anti-mouse CD326 (EpCAM) antibodies were purchased from Bio Legend (San Diego, CA, USA). Rat anti-mouse anti-CD45 antibody was purchased from BioLegend (San Diego CA). 1,1′-dioctadecyl-3,3,3′3′-tetramethylindocarbocyanine perchlorate (DiI) was from Biotium (Hayward, CA). Anti-CD20 humanized antibody (Rituximab, Genentech) was obtained from UCSD Moores Cancer Center Pharmacy. AlexaFluor 488 goat anti-rat antibody and AlexaFluor 488 goat anti-human antibody were purchased from Life Technologies (Carlsbad, CA). FITC mouse anti-human CD19 (clone HIB19) antibody and FITC mouse anti-human CD45 (Clone H130) antibody were from BD Biosciences (La Jolla, CA). FITC labeled rat anti mouse CD45 antibody and FITC labeled rituximab antibody were synthesized in the laboratory.
Mukthavaram R., Shi G., Kesari S, & Simberg D. (2014). Targeting and depletion of circulating leukocytes and cancer cells by lipophilic antibody-modified erythrocytes. Journal of controlled release : official journal of the Controlled Release Society, 183, 146-153.
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