Cd11b

Manufactured by Wuhan Servicebio Technology

Sourced in China

CD11b is a cell surface glycoprotein that functions as an integrin alpha subunit. It is commonly expressed on the surface of myeloid cells, including monocytes, macrophages, and neutrophils, and plays a role in cell-cell and cell-extracellular matrix interactions.

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Lab products found in correlation

F4 80, by Wuhan Servicebio Technology (2 mentions) Caseviewer software, by 3DHISTECH (2 mentions) Pannoramic midi 2, by 3DHISTECH (2 mentions) Lsm 780, by Zeiss (1 mentions) Gb23303, by Wuhan Servicebio Technology (1 mentions) Ab289542, by Abcam (1 mentions) Gb113151, by Wuhan Servicebio Technology (1 mentions) Gb25303, by Wuhan Servicebio Technology (1 mentions) Gb11181, by Wuhan Servicebio Technology (1 mentions) Cleaved caspase 3, by Cell Signaling Technology (1 mentions) Eclipse e600 microscope, by Nikon (1 mentions) Apoptag peroxidase in situ apoptosis detection kit, by Merck Group (1 mentions) Ultravision quanto detection system hrp, by Thermo Fisher Scientific (1 mentions) Il 17a, by Abcam (1 mentions) Confocal microscope, by Nikon (1 mentions) 4 6 diamidino 2 phenylindole (dapi), by Merck Group (1 mentions) Goat anti rabbit igg, by Wuhan Servicebio Technology (1 mentions) Anti pdpn, by Proteintech (1 mentions) Rhodamine phalloidin, by Beyotime (1 mentions) Il 10, by Wuhan Servicebio Technology (1 mentions)

Spelling variants of this product

Anti-CD11b antibody (2 citations)

9 protocols using cd11b

Immunohistochemical Macrophage and Neutrophil Profiling

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IHC was performed for both macrophage and neutrophil infiltrations using CD11b and Ly6G antibodies (Servicebio, China) [26 (link)].

Yu Z., Tong Y., Zhang R., Ding X, & Li Q. (2017). Saquinavir Ameliorates Liver Warm Ischemia-Reperfusion-Induced Lung Injury via HMGB-1- and P38/JNK-Mediated TLR-4-Dependent Signaling Pathways. Mediators of Inflammation, 2017, 7083528.

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Immunohistochemical Analysis of Adipose Tissue

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Fresh adipose tissues were obtained, fixed, and embedded in paraffin. After antigen repair and hydrogen peroxide blocking, the slides were blocked with a solution containing 3% BSA before incubation with primary antibodies at 4°C overnight. The slides were then treated with horseradish peroxidase (HRP)-conjugated anti-rabbit (Servicebio, GB23303, 1:500) or Alexa Fluor 488-conjugated anti-rabbit (Servicebio, GB25303, 1:400) secondary antibodies for 50 min at room temperature. The primary antibodies included CD44 (Servicebio, GB112054, 1:3000), CD62L (Bioss, bs-1036R, 1:1000), CD31 (Servicebio, GB113151, 1:1000), TH (Servicebio, GB11181, 1:1000), CD11B (Service bio, GB11058, 1:3000), CD115 (Servicebio, GB11581, 1:1000), and NK1.1 (Abcam, AB289542, 1:100). All the fluorescence pictures were captured by a fluorescence microscope (Nikon Eclipse C1) or laser scanning confocal microscope (Zeiss LSM 780).

Ye Y., Wang H., Chen W., Chen Z., Wu D., Zhang F, & Hu F. (2024). Dynamic changes of immunocyte subpopulations in thermogenic activation of adipose tissues. Frontiers in Immunology, 15, 1375138.

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Immunohistochemical Analysis of Tumor Samples

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Sections of formalin-fixed, paraffin-embedded tumor specimens were deparaffinized in xylene and hydrated in a graded alcohol series and then immersed the slides in sodium citrate antigen retrieval solution for antigen retrieval at 100 °C for 20 min. Endogenous peroxidase was blocked using 3% hydrogen peroxide in distilled water for 15 min. The tissue slides were incubated with 3% BSA solution (Servicebio, Beijing China) for 15 min at room temperature. Formalin-fixed and paraffin-embedded tissue sections (3–4 mm) were incubated with the following Abs: Ki67, CD20, CD11b, F4/80 (Servicebio) and cleaved-caspase-3 (Cell Signaling Technology, Danvers, MA, USA). UltraVisionQuanto Detection System HRP (Thermo Fisher Scientific Inc.,Waltham, MA, USA) and DAB (Liquid DABþ Substrate Chromogen System, Dako, Copenhagen, Denmark) were used to develop the reaction. TUNEL staining (ApopTag Peroxidase In Situ Apoptosis Detection Kit; Merck Millipore) was performed according to the manufacturer’s instructions. Images were acquired by AperioScanScope XT systems (Aperio Technologies, Leica Microsystems Srl, Wetzlar, Germany) or Eclipse E600 microscope (Nikon, Tokyo, Japan).

Ke C., Hou H., Li J., Su K., Huang C., Lin Y., Lu Z., Du Z., Tan W, & Yuan Z. (2020). Extracellular Vesicle Delivery of TRAIL Eradicates Resistant Tumor Growth in Combination with CDK Inhibition by Dinaciclib. Cancers, 12(5), 1157.

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Histological Analysis of Skin Inflammation

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Biopsied tissue was fixed in 4% formalin, embedded in paraffin, sectioned at 4 μm, and stained with hematoxylin and eosin (H&E) and toluidine blue (TB). Infiltrated lymphocytes, thickening of the epidermis, and fibrosis in the dermis were observed using H&E-stained tissue sample under a magnification of × 400. Thickness was measured in five randomly selected fields from each sample. Mast cell infiltration was measured by counting the number of mast cell in four sites chosen at random in biopsies stained with toluidine blue at a magnification of × 400. For immunohistochemical staining, sections were blocked with 10% BSA for 2 h, followed by overnight incubation with a primary antibody against IL-17A (Abcam), CD11b (Service Bio, Wuhan) and CK14 (Service Bio, Wuhan) at 4 °C. Subsequently, sections were washed and incubated with horseradish peroxidase-conjugated secondary antibodies for 1 h at room temperature. All stained skin sections were observed using an inverted microscope (IX73, Olympus, Japan).

Dong H., Feng C., Cai X., Hao Y., Gu X., Cai L., Wu S., Chen J., Liu Z., Xie W., Lu X., Qian H., Liu Y., Cao Y., Zhu J., Xu J., Zhou Y., Ma S., Yang S., Shi Y., Yu H., Shi M., Wang Y., Gu H.F., Fan L, & Wu L. (2022). 7-Methoxyisoflavone ameliorates atopic dermatitis symptoms by regulating multiple signaling pathways and reducing chemokine production. Scientific Reports, 12, 8760.

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Immunofluorescence Staining of Tissue Samples

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The samples were washed with PBS, fixed with 4 % paraformaldehyde for 30 min, according to experimental requirements, were incubated with anti-Pdpn (proteintech, 1:200), anti-Grem1 (CST, 1:50), anti-Cd200 (proteintech, 1:200), anti-Ctsk (Abcam, 1:200), anti-Ptgs2 (proteintech, 1:200), anti-vinculin (proteintech, 1:200) or anti-YAP (CST, 1:200) at 4 °C for 8–12 h. After that, samples incubated with goat anti-rabbit IgG (Servicebio, Alexa Fluor 488, 1:300), goat anti-rabbit IgG (Servicebio, Cy3, 1:300) or goat anti-mouse IgG (Servicebio, Alexa Fluor 488, 1:200) for 1 h at room temperature. Nuclear staining was conducted with DAPI (Sigma), and F-actin stained by rhodamine phalloidin (Beyotime). Tissue samples were fixed in 4 % paraformaldehyde. After decalcification, all samples were embedded in paraffin and sectioned at 6-μm slices. The tissue sections were immunofluorescently stained with primary antibodies targeting Sry (Santa Cruz, 1:200), CD11b (Servicebio, 1:500), TNF-α (Uscn Life Science Inc., 1:1000), IL-1β (Servicebio, 1:1200) and IL-10 (Servicebio, 1:1000), and the goat anti-rabbit IgG (Servicebio, Alexa Fluor 488, 1:400) and goat anti-rabbit IgG (Servicebio, Cy3, 1:500). DAPI was used to stain the nuclei. The images were observed by a fluorescence microscope (Olympus CKX53) or Nikon confocal microscope (Nikon).

Li P.L., Chen D.F., Li X.T., Hao R.C., Zhao Z.D., Li Z.L., Yin B.F., Tang J., Luo Y.W., Wu C.T., Nie J.J, & Zhu H. (2023). Microgel-based carriers enhance skeletal stem cell reprogramming towards immunomodulatory phenotype in osteoarthritic therapy. Bioactive Materials, 34, 204-220.

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Immunohistochemical Analysis of Macrophage Markers

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The paraffin-embedded sections were deparaffinized and rehydrated. The sections were incubated with primary antibodies against F4/80 (Cell Signaling Technology, MA, USA, Cat# 70076T; 1:100 dilution), and CD11b (Servicebio, Wuhan, China, Cat# GB11058; 1:100 dilution) for 2 h at room temperature and washed three times with PBS. Then, the sections were incubated with horseradish peroxidase-conjugated secondary antibodies (Servicebio, Wuhan, China, Cat# GB23303, 1:200 dilution) for 1 h at room temperature, followed by 3,3’-diaminobenzidine (DAB) development, hematoxylin staining, dehydration, and mounting.

Li D.P., Huang L., Kan R.R., Meng X.Y., Wang S.Y., Zou H.J., Guo Y.M., Luo P.Q., Pan L.M., Xiang Y.X., Mao B.B., Xie Y.Y., Wang Z.H., Yang M., He R., Yang Y., Liu Z.L., Xie J.H., Ma D.L., Zhang B.P., Shao S.Y., Chen X., Xu S.M., He W.T., Li W.J., Chen Y, & Yu X.F. (2023). LILRB2/PirB mediates macrophage recruitment in fibrogenesis of nonalcoholic steatohepatitis. Nature Communications, 14, 4436.

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Histological and Immunohistochemical Analysis of Liver

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Liver samples were immediately fixed with 4% paraformaldehyde and then embedded in paraffin. For histological analysis, liver sections (5 μm) were stained with H&E to assess liver granulomas and Masson’s trichrome to assess liver fibrosis. For Immunohistochemical analysis, CD11b and CD19 staining were performed according to standard procedures. In briefly, sections were incubated with CD11b (Servicebio, China; Cat No.: GB11058) or CD19 (Servicebio, China; Cat No.: GB11061-1) overnight and the next day stained with secondary antibody. The nucleus was stained with hematoxylin and then treated with diaminobenzidine. Images were acquired on a Pannoramic MIDI II (3D HISTECH). The positive areas were measured by using CaseViewer software (3D HISTECH).

Lu L., Hu J., Chao T., Chen Z., Liu Z., Luo X., Liang Y., He P, & Zhang L. (2020). Loss of natural resistance to schistosome in T cell deficient rat. PLoS Neglected Tropical Diseases, 14(12), e0008909.

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Histopathological Analysis of Liver Tissue

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H&E and Oil red O staining were conducted as previously reported. [1] Briefly, liver tissues fixed with 10% neutral-buffered formalin were cut into 7 μm-thick sections, followed by routine H&E, Oil Red O, and Sirius staining. Histological analysis was performed by an experienced hepatopathologist from the First Affiliated Hospital of Chinese Medicine using a light microscope (Olympus). Immunofluorescence was conducted at Servicebio (Wuhan, China) according to the previous protocols. Liver sections or MPHs were incubated at 4 °C overnight with primary antibodies against F4/80 (Servicebio, China), CD11b (Servicebio, China), α-SMA (Abmart, China), FXR (Abcam, USA), Bsep (Abmart, China) and Zbtb18 (Proteintech, China), followed by incubation with indicated secondary antibodies. To detect lipid droplets, liver sections were incubated with BODIPY 493/503 (Thermofisher Scientific) for 30 min. All fluorescence images were obtained using a confocal laser scanning microscope (Leica) or a light microscope (Olympus).

Zhang L., Chen J., Yang X., Shen C., Huang J., Zhang D., Liu N., Liu C., Zhong Y., Chen Y., Tang K., Guo J., Cui T., Duan S., Li J., Huang S., Pan H., Zhang H., Tang X., Chang Y, & Gao Y. (2024). Hepatic Zbtb18 (Zinc Finger and BTB Domain Containing 18) alleviates hepatic steatohepatitis via FXR (Farnesoid X Receptor). Signal Transduction and Targeted Therapy, 9, 20.

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Liver Histopathology and Immune Cell Analysis

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Liver samples were collected and immediately fixed in 4% paraformaldehyde. Liver sections were stained with H&E and Masson’s trichrome for analysis of liver granulomas and liver fibrosis, respectively. CD45 (Servicebio, GB11066), CD11b (Servicebio, GB11058) and CD19 (Servicebio, GB11061-1) staining were performed for immunohistochemical analysis, according to standard procedures as our previously study described[9 (link)]. Images were scanned by Pannoramic MIDI II (3D HISTECH) and analyzed with HALO image analysis software (Indica Labs) and CaseViewer software (3D HISTECH).

Lu L., Li T., Feng X., Liu Z., Liu Y., Chao T., Gu Y., Huang R., Zhang F., He L., Zhou B., Kong E., Liu Z., Wang X., Chen Z., Wang H., Malissen M., Malissen B., Zhang L, & Liang Y. (2022). Excessive immunosuppression by regulatory T cells antagonizes T cell response to schistosome infection in PD-1-deficient mice. PLoS Pathogens, 18(6), e1010596.

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