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Application suite x

Manufactured by Leica Microsystems
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Application Suite X is a software package for Leica Microsystems' imaging and analysis equipment. It provides a platform for capturing, processing, and analyzing microscopic images and data. The core function of Application Suite X is to enable users to perform a variety of image-based tasks, such as acquisition, visualization, and quantification, across a range of Leica Microsystems' product lines.

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Lab products found in correlation

Tcs sp8, by Leica Microsystems (2 mentions) Tcs sp8 mp multiphoton imaging system, by Leica Microsystems (2 mentions) Vectashield medium, by Vector Laboratories (2 mentions) Tcs sp8 system, by Leica Microsystems (1 mentions) Quantify one, by Bio-Rad (1 mentions) Pierce elc western blotting substrate, by Thermo Fisher Scientific (1 mentions) Chemidoc xr, by Bio-Rad (1 mentions)

6 protocols using application suite x

1

Quantifying Oxidative Stress in Cell Lines

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MCF7 or U373 cells cultured in 120-mm coverslips were exposed to 30 µM DCFH-DA (0.1 M PB, pH 7.4) and fixed in 4% paraformaldehyde for 20 min. For fluorescence detection, coverslips were mounted on glass slides and observed with a laser-scanning confocal microscope (TCS SP8 System and Application suite X, Leica Microsystems). DAPI and fluorescein phalloidin (nuclei and cytoskeleton detection, respectively) staining was also used.
Muñoz D., Brucoli M., Zecchini S., Sandoval-Hernandez A., Arboleda G., Lopez-Vallejo F., Delgado W., Giovarelli M., Coazzoli M., Catalani E., Palma C.D., Perrotta C., Cuca L., Clementi E, & Cervia D. (2019). XIAP as a Target of New Small Organic Natural Molecules Inducing Human Cancer Cell Death. Cancers, 11(9), 1336.
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2

Confocal Microscopy Immunostaining Protocol

Cited 1 time
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Immunostaining was performed as previously described (Takeuchi et al., 2019, PLoS Pathog) [12 (link)]. Images were acquired with a confocal laser microscope (TCS SP8; Leica Microsystems) using a 64× oil-immersion object lens with a numerical aperture of 1.4. Acquired images were analyzed using the Application Suite X software package (Leica Microsystems).
Takeuchi H., Kato Y., Sasaki N., Tanigaki K., Yamaga S., Mita E., Kuboniwa M., Matsusaki M, & Amano A. (2022). Surface pre-reacted glass-ionomer eluate protects gingival epithelium from penetration by lipopolysaccharides and peptidoglycans via transcription factor EB pathway. PLoS ONE, 17(7), e0271192.
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3

Immunoblotting and Immunocytochemistry Protocols

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Immunoblotting and immunocytochemistry were performed as previously described10 (link). Immunoreactive bands were detected using Pierce ELC Western Blotting Substrate (Thermo Scientific) and ChemiDoc XRS (Bio Rad), and images were acquired using the Quantify One software package (Bio-Rad). Confocal microscopic images were acquired with a confocal laser microscope (TCS SP8; Leica Microsystems) using a 64 × oil-immersion object lens with a numerical aperture of 1.4, then analyzed using the Application Suite X software package (Leica Microsystems).
Yamaga S., Tanigaki K., Nakamura E., Sasaki N., Kato Y., Kuboniwa M., Matsusaki M., Amano A, & Takeuchi H. (2023). Cigarette smoke extract impairs gingival epithelial barrier function. Scientific Reports, 13, 9228.
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4

Retinal Imaging and Analysis

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Retinal cross sections and flat-mount retinas were imaged with DM6000 Upright Microscope System and TCS SP5 confocal microscope (Leica Microsystems), respectively. Adobe Photoshop CS6 Extended (Adobe Systems), Application Suite X (Leica Microsystems), ImageJ (https://imagej.nih.gov/ij/), or Imaris 7.6.1 (Bitplane) were used for image analysis, 3D reconstruction, and movie production.
Nagashima M., D'Cruz T.S., Danku A.E., Hesse D., Sifuentes C., Raymond P.A, & Hitchcock P.F. (2020). Midkine-a Is Required for Cell Cycle Progression of Müller Glia during Neuronal Regeneration in the Vertebrate Retina. The Journal of Neuroscience, 40(6), 1232-1247.
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5

Quantification of Retinal Retinosomes

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Enucleated mouse eyes from dark-adapted (overnight) or daylight-adapted (≥ 1 h) animals were rinsed in PBS and incubated for 5 min at RT in freshly prepared fixing solution: PBS with 4% paraformaldehyde. Dark-adapted eyes were processed under dim red light and dissected using night vision goggles. Following dissection along the posterior margin of the limbus, lens and vitreous were removed and four radial cuts were made toward the optic nerve to flatten the remaining eyecups on glass slides. The samples were then mounted with Vectashield medium (Vector Laboratories) for imaging. To image tissue from pigmented animals, a customized TCS SP8 MP multiphoton imaging system (Leica Microsystems) with a pulse selection system delivering 75 fsec pulses at 8 MHz was used.29 (link) Phasor analyses of FLIM data in Application Suite X (Leica Microsystems) were used to confirm identity of fluorescing compounds and determine their subcellular distribution.29 (link) ImageJ analyze-particles software (NIH) was used to quantify the area occupied by retinosomes.
Tworak A., Kolesnikov A.V., Hong J.D., Choi E.H., Luu J.C., Palczewska G., Dong Z., Lewandowski D., Brooks M.J., Campello L., Swaroop A., Kiser P.D., Kefalov V.J, & Palczewski K. (2023). Rapid RGR-dependent visual pigment recycling is mediated by the RPE and specialized Müller glia. Cell reports, 42(8), 112982.
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6

Quantification of Retinal Retinosomes

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Enucleated mouse eyes from dark-adapted (overnight) or daylight-adapted (≥ 1 h) animals were rinsed in PBS and incubated for 5 min at RT in freshly prepared fixing solution: PBS with 4% paraformaldehyde. Dark-adapted eyes were processed under dim red light and dissected using night vision goggles. Following dissection along the posterior margin of the limbus, lens and vitreous were removed and four radial cuts were made toward the optic nerve to flatten the remaining eyecups on glass slides. The samples were then mounted with Vectashield medium (Vector Laboratories) for imaging. To image tissue from pigmented animals, a customized TCS SP8 MP multiphoton imaging system (Leica Microsystems) with a pulse selection system delivering 75 fsec pulses at 8 MHz was used.29 (link) Phasor analyses of FLIM data in Application Suite X (Leica Microsystems) were used to confirm identity of fluorescing compounds and determine their subcellular distribution.29 (link) ImageJ analyze-particles software (NIH) was used to quantify the area occupied by retinosomes.
Tworak A., Kolesnikov A.V., Hong J.D., Choi E.H., Luu J.C., Palczewska G., Dong Z., Lewandowski D., Brooks M.J., Campello L., Swaroop A., Kiser P.D., Kefalov V.J, & Palczewski K. (2023). Rapid RGR-dependent visual pigment recycling is mediated by the RPE and specialized Müller glia. Cell reports, 42(8), 112982.
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