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Anti muc1 c hm 1630 p1abx

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The Anti-MUC1-C (HM-1630-P1ABX) is a laboratory reagent used for research purposes. It functions as an antibody that can bind to the MUC1-C protein. This product is intended for in vitro use only.

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Lab products found in correlation

Anti β actin, by Merck Group (12 mentions) Gel extraction columns, by Qiagen (3 mentions) Abi prism 7300 sequence detector, by Thermo Fisher Scientific (3 mentions) Magnetic dynabeads, by Thermo Fisher Scientific (3 mentions) Control non immune igg, by Santa Cruz Biotechnology (3 mentions) Power sybr green pcr master mix, by Thermo Fisher Scientific (3 mentions) Ab110641, by Abcam (3 mentions) Anti oct4, by Cell Signaling Technology (3 mentions) Anti sox2, by Cell Signaling Technology (3 mentions) Anti jun, by Cell Signaling Technology (2 mentions) Anti bmi1, by Cell Signaling Technology (2 mentions) Anti ezh2, by Cell Signaling Technology (2 mentions) Anti brg1, by Abcam (2 mentions) Ab175186, by Abcam (2 mentions) Ab32072, by Abcam (2 mentions) Anti klf4, by Cell Signaling Technology (2 mentions) Anti myc, by Abcam (2 mentions) Protease inhibitor cocktail, by Thermo Fisher Scientific (2 mentions) Anti arid1a, by Cell Signaling Technology (1 mentions) Anti notch1, by Cell Signaling Technology (1 mentions)

15 protocols using anti muc1 c hm 1630 p1abx

1

Immunoblotting of Cellular Signaling Proteins

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Total lysates prepared from subconfluent cells were immunoblotted with anti-MUC1-C (HM-1630-P1ABX, 1:400 dilution; Thermo Fisher Scientific), anti-JUN (3742, 1:1,000; Cell Signaling Technology), anti-ARID1A (12354, 1:1,000; Cell Signaling Technology), anti-NOTCH1 (3608, 1:1,000; Cell Signaling Technology), anti-β-actin (A5441, 1:100,000; Sigma), and anti-GAPDH (5174, 1:5,000; Cell Signaling Technology).
Bhattacharya A., Fushimi A., Yamashita N., Hagiwara M., Morimoto Y., Rajabi H., Long M.D., Abdulla M., Ahmad R., Street K., Liu S., Liu T, & Kufe D. (2022). MUC1-C Dictates JUN and BAF-Mediated Chromatin Remodeling at Enhancer Signatures in Cancer Stem Cells. Molecular Cancer Research, 20(4), 556-567.
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2

Immunoblotting Analysis of DNA Damage Signaling

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Lysates were subjected to immunoblotting with anti-MUC1-C (HM-1630-P1ABX; Thermo Fisher Scientific), anti-β-actin (A5441; Sigma), anti-lamin A/C (4777), anti-γH2AX (9718), anti-ATR (2790), anti-pCHK1 (2348), anti-CHK1 (2360), anti-pBRCA1 (9009), anti-BRCA1 (14823), anti-BMI1 (6964), anti-H2AUb1 (8240), anti-H2A (12349), anti-EZH2 (5246), anti-H3K27me3 (9733), anti-H3 (9715), anti-H3K56ac (4243), anti-PARP1 (9532) (Cell Signaling Technology, Danvers, MA, USA), anti-pATR (GTX128145; GeneTex, Irvine, CA, USA) and anti-FANCD2 (ac108928; Abcam, Cambridge, CB4, 0Fl, UK). Signal intensity was determined using ImageJ 1.51k software (NIH, Bethesda, MD, USA).
Yamamoto M., Jin C., Hata T., Yasumizu Y., Zhang Y., Hong D., Maeda T., Miyo M., Hiraki M., Suzuki Y., Hinohara K., Rajabi H, & Kufe D. (2019). MUC1-C INTEGRATES CHROMATIN REMODELING AND PARP1 ACTIVITY IN THE DNA DAMAGE RESPONSE OF TRIPLE-NEGATIVE BREAST CANCER CELLS. Cancer research, 79(8), 2031-2041.
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3

Chromatin Immunoprecipitation Assay Protocol

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Formaldehyde cross-linked and sheered soluble chromatin was precipitated with pre-cleared magnetic dynabeads (ThermoFisher Scientific) and 2 μg/ml of anti-MUC1-C (HM-1630-P1ABX; ThermoFisher Scientific), anti-E2F1 (3742; CST), anti-STAT3 (9139; CST), anti-BRG1 (ab110641; abcam), anti-ARID1A (12354, 2 μg/ml; CST) or a control non-immune IgG (Santa Cruz Biotechnology). The DNA-antibody precipitates were reverse cross-linked at 65°C for 18 h. DNAs were purified using gel extraction columns (QIAGEN, Germantown, MD, USA) and analyzed by qPCR using the Power SYBR Green PCR Master Mix and the ABI Prism 7300 sequence detector (Applied Biosystems). Data are reported as relative fold enrichment (10 (link)). Primers used for ChIP qPCR are listed in Supplementary Table 2.
Hagiwara M., Yasumizu Y., Yamashita N., Rajabi H., Fushimi A., Long M.D., Li W., Bhattacharya A., Ahmad R., Oya M., Liu S, & Kufe D. (2020). MUC1-C ACTIVATES THE BAF (mSWI/SNF) COMPLEX IN PROSTATE CANCER STEM CELLS. Cancer research, 81(4), 1111-1122.
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4

Immunoblot Analysis of Cellular Proteins

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Total lysates prepared from non-confluent cells were subjected to immunoblot analysis using anti-MUC1-C (HM-1630-P1ABX, 1:1000 dilution; Thermo Fisher Scientific), anti-β-actin (A5441, 1:5000 dilution; Sigma-Aldrich), anti-GAPDH (#2118, 1:1000; CST), anti-NF-κB p65 (ab32536, 1:1000 dilution, Abcam, Cambridge, MA, USA), anti-GPX4 (#52455, 1:1000 dilution, CST), anti-GSR (18257-1-AP, 1:2000 dilution; PROTEINTECH, Rosemont, IL, USA), anti-LRP8 (NB100-2216, 1:1000 dilution; Novus Biologicals, Centennial, CO, USA) and anti-CD133 (#5860, 1:1000 dilution, CST).
Daimon T., Bhattacharya A., Wang K., Haratake N., Nakashoji A., Ozawa H., Morimoto Y., Yamashita N., Kosaka T., Oya M, & Kufe D.W. (2024). MUC1-C is a target of salinomycin in inducing ferroptosis of cancer stem cells. Cell Death Discovery, 10, 9.
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5

Immunoblotting Analysis of Stem Cell Markers

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Total lysates prepared from subconfluent cells were immunoblotted with anti-MUC1-C (HM-1630-P1ABX, 1:400 dilution; ThermoFisher Scientific, Waltham, MA, USA), anti-PBRM1 (A301–591A, 1:10000; Bethyl Laboratories, Montgomery, TX, USA), anti-ARID2 (82342, 1:1000; Cell Signaling Technology (CST), Danvers, MA, USA), anti-BRD7 (15125, 1:1000; CST), anti-β-actin (A5441, 1:100,000; Sigma), anti-E2F1 (3742, 1:1000; CST), anti-SLC7A11/xCT (ab175186, 1:1000; abcam, Cambridge, MA, USA), anti-G6PD (8866, 1:1000; CST), anti-PGD (13389, 1:1000; CST), anti-BRG1 (ab110641, 1:10000; abcam, Cambridge, MA, USA), anti-ARID1A (12354, 1:500; CST), anti-OCT4 (2750, 1:1000 dilution; Cell Signaling Technology), anti-SOX2 (3579, 1:1000 dilution; Cell Signaling Technology), anti-KLF4 (12173, 1:1000 dilution; Cell Signaling Technology), anti-MYC (ab32072, 1:1000 dilution; Abcam, Cambridge, MA), anti-NANOG (4903, 1:1000; CST), anti-NOTCH1 (3608. 1:1000; CST), anti-BMI1 (6964, 1:1000, CST), anti-CD44 (KO601, 1:1000; TransGenic, Tokyo, Japan), anti-CD133 (5860, 1:1000; CST) and anti-GAPDH (5174, 1:5000, CST).
Hagiwara M., Fushimi A., Yamashita N., Bhattacharya A., Rajabi H., Long M.D., Yasumizu Y., Oya M., Liu S, & Kufe D. (2021). MUC1-C activates the PBAF chromatin remodeling complex in integrating redox balance with progression of human prostate cancer stem cells. Oncogene, 40(30), 4930-4940.
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6

Immunoblot Analysis of Cell Proteins

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Total protein lysates from cultured cells were subjected to immunoblot analysis using anti-MUC1-C (HM-1630-P1ABX, 1:100 dilution; Thermo Fisher Scientific), anti-MICA (ab150355, 1:1000 dilution; Abcam), anti-MICB (77 296S, 1:1000 dilution; Cell Signaling Technology (CST)), anti-β-actin (A5441, 1:5000 dilution; Sigma-Aldrich), anti-CD9 (13 174S, 1:1000 dilution; CST), anti-CD63 (ab59479, 1:1000 dilution; Abcam), anti-CD81 (56 039S, 1:1000 dilution; CST), anti-ERp5 (1:2500; 18 233–1-AP, Proteintech, Rosemont, Illinois, USA) and anti-RAB27A (69 295S, 1:1000 dilution; CST).
Morimoto Y., Yamashita N., Daimon T., Hirose H., Yamano S., Haratake N., Ishikawa S., Bhattacharya A., Fushimi A., Ahmad R., Takahashi H., Dashevsky O., Mitsiades C, & Kufe D. (2023). MUC1-C is a master regulator of MICA/B NKG2D ligand and exosome secretion in human cancer cells. Journal for Immunotherapy of Cancer, 11(2), e006238.
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7

Immunoblotting and Immunoprecipitation of Nuclear Proteins

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Total and nuclear lysates prepared from subconfluent cells were subjected to immunoblot analysis using anti-MUC1-C (#HM-1630-P1ABX; Thermo Fisher Scientific, Waltham, MA, USA), anti-MYC (#ab32072; Abcam), anti-ERα (#ab108398; Abcam) anti-β-actin (#A5441; Sigma), anti-MTA1 (#5647), anti-MBD3 (#14540), anti-CHD4 (#11912), anti-HDAC1 (#5356), anti-SOX2 (#D6D9), anti-KLF4 (#D1F2), anti-BMI1 (#D20B7), anti-CD44 (#156-3C11), anti-OCT4 (#2750S; Cell Signaling Technology, Danvers, MA, USA). Nuclear proteins were immunoprecipitated in the absence and presence of 50 μg/ml ethidium bromide (EtBr; #15585-011, Thermo Fisher Scientific) as described (25 (link)).
Hata T., Rajabi H., Takahashi H., Yasumizu Y., Li W., Jin C., Long M.D., Hu Q., Liu S., Fushimi A., Yamashita N., Kui L., Hong D., Yamamoto M., Miyo M., Hiraki M., Maeda T., Suzuki Y., Samur M.K, & Kufe D. (2019). MUC1-C ACTIVATES THE NuRD COMPLEX TO DRIVE DEDIFFERENTIATION OF TRIPLE-NEGATIVE BREAST CANCER CELLS. Cancer research, 79(22), 5711-5722.
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8

Immunoblotting of Cancer Stem Cell Markers

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Whole cell and nuclear lysates were prepared in RIPA buffer containing protease inhibitor cocktail (Thermo Scientific, Waltham, MA, USA). Immunoblotting was performed with anti-MUC1-C (HM-1630-P1ABX; Thermo Fisher Scientific), anti-TWIST1 (ab50887), anti-NF-κB p65 (ab16502), anti-ALDH1 (ab134188) (Abcam, Cambridge, MA, USA), anti-pSTAT3 (#9145), anti-STAT3 (#9139), anti-ZEB1 (#3396), anti-SNAIL (#3879), anti-SOX2 (#3579), anti-ABCB1 (#13342), anti-N-cadherin (13116S), anti-BMI1 (6964P), anti-CD44 (5640S), anti-GAPDH (5174S) (Cell Signaling Technology, Danvers, MA, USA) and anti-β-actin (A5441; Sigma).
Hata T., Rajabi H., Yamamoto M., Jin C., Ahmad R., Zhang Y., Kui L., Li W., Yasumizu Y., Hong D., Miyo M., Hiraki M., Maeda T., Suzuki Y., Takahashi H., Samur M, & Kufe D. (2019). TARGETING MUC1-C INHIBITS TWIST1 SIGNALING IN TRIPLE-NEGATIVE BREAST CANCER. Molecular cancer therapeutics, 18(10), 1744-1754.
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9

Chromatin Immunoprecipitation Assay for Transcription Factors

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Formaldehyde cross-linked and sheered soluble chromatin was precipitated with pre-cleared magnetic dynabeads (ThermoFisher Scientific) and 2 μg/ml of anti-MUC1-C (HM-1630-P1ABX; ThermoFisher Scientific), anti-E2F1 (3742; CST), anti-NRF2 (12721; CST), anti-PBRM1 (8183; CST) or a control non-immune IgG (Santa Cruz Biotechnology). The DNA-antibody precipitates were reverse cross-linked at 65oC for 18 h. DNAs were purified using gel extraction columns (QIAGEN, Germantown, MD, USA) and analyzed by qPCR using the Power SYBR Green PCR Master Mix and the ABI Prism 7300 sequence detector (Applied Biosystems). Data are reported as relative fold enrichment 12. Primers used for ChIP qPCR are listed in Supplementary Table 2.
Hagiwara M., Fushimi A., Yamashita N., Bhattacharya A., Rajabi H., Long M.D., Yasumizu Y., Oya M., Liu S, & Kufe D. (2021). MUC1-C activates the PBAF chromatin remodeling complex in integrating redox balance with progression of human prostate cancer stem cells. Oncogene, 40(30), 4930-4940.
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10

Immunoblotting of Cell Lysates

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Total lysates prepared from subconfluent cells were immunoblotted with anti-MUC1-C (HM-1630-P1ABX, 1:400 dilution; Thermo Fisher Scientific), anti-JUN (3742, 1:1000; Cell Signaling Technology (CST), Danvers, MA, USA), anti-ARID1A (12354, 1:1000; CST), anti-NOTCH1 (3608, 1:1000; CST), anti-β-actin (A5441, 1:100,000; Sigma) and anti-GAPDH (5174, 1:5000; CST).
Bhattacharya A., Fushimi A., Yamashita N., Hagiwara M., Morimoto Y., Rajabi H., Long M.D., Abdulla M., Ahmad R., Street K., Liu S., Liu T, & Kufe D. (2022). MUC1-C Dictates JUN and BAF-Mediated Chromatin Remodeling at Enhancer Signatures in Cancer Stem Cells. Molecular Cancer Research, 20(4), 556-567.
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