786-0 cells were first treated with DMF at 150uM for a duration of 4 hours then infected with HSV at an MOI of 0.1. The 786-0 cell supernatant, obtained 24 hours post-infection following treatment and infection, underwent assessment for the concentration of human IFN-β. This quantification was conducted using the Human IFN Beta ELISA Kit (PBL Assay Science, cat. 41410), adhering to the guidelines provided by the manufacturer. Absorbance readings were taken using BioTek Cytation C10 Confocal Imaging Reader.
Human ifn beta elisa kit
Manufactured by PBL Assay Science
The Human IFN Beta ELISA Kit is a quantitative sandwich enzyme-linked immunosorbent assay (ELISA) designed for the measurement of human interferon beta (IFN-β) levels in biological samples such as cell culture supernatants, plasma, and serum.
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5 protocols using human ifn beta elisa kit
1
Quantification of IFN-β in HSV-infected 786-0 Cells
2
Quantifying IFNβ and IFNL2/3
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IFNβ and IFNL2/3 contained in the supernatant of cells were quantified using the human IFN-beta ELISA kit and DIY IFNLR 2/3 ELISA kit both from PBL-Interferon Source, per manufacturer’s instructions.
3
IFNB1 Secretion Quantification
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The amount of IFNB1 secreted into the growth medium was determined using a Human IFN beta ELISA kit (PBL Assay Science). The medium from RNP-transfected cells was collected at either 24 h or 3 d post-transfection. The IFNB1 concentration was measured according to the manufacturer's protocol.
4
Cytokine Profiling by ELISA
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Cell-free supernatants were analyzed by using Human IFN-alpha Platinum ELISA (eBioscience), human IFN Beta ELISA kit (PBL), and human IL-12, IL-10 and TNF-α ELISA (BioLegend) according to the manufacturer’s instructions.
5
Interferon Beta Secretion Assay
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Primary MEFs and human tumor cell lines were seeded in 96-well plates at a density of 15,000 cells/well. Approximately 15 hours post-seeding, cells were exposed to IR. Cell culture supernatant were harvested 48 hours post-IR and assayed for IFN-beta using the VeriKine-HS Mouse Interferon Beta Serum ELISA Kit (PBL Assay Science) and the Human IFN-beta ELISA kit for murine and human cell lines following the manufacturer's protocol. Absorbance at 450 nm was measured using a BioTek Synergy HT plate reader. IFN-beta concentration was calculated by using standard concentration and fitting a five-parameter logistic non-linear regression model available at the free analysis software ELISA Analysis (http://www.elisaanalysis.com/ ).
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