Sierk

Manufactured by Santa Cruz Biotechnology

Sourced in United States

siERK is a small interfering RNA (siRNA) that targets the extracellular signal-regulated kinase (ERK) gene. It is designed to reduce the expression of the ERK protein in cells.

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Lab products found in correlation

P erk, by Santa Cruz Biotechnology (1 mentions) Sinrf2, by Santa Cruz Biotechnology (1 mentions) Lipopolysaccharides (lps), by Merck Group (1 mentions) Transfection reagent, by Santa Cruz Biotechnology (1 mentions)

3 protocols using sierk

Molecular Mechanisms of Nrf2 and ERK Regulation

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Gln, H₂O₂, LPS, and 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-tetrazolium bromide (MTT) were purchased from Sigma-Aldrich Chemical Co. (St. Louis, MO, USA). Antibodies against iNOS, Nrf2, HO-1, ERK, phospho-ERK (p-ERK), and β-actin, and silencing RNA (siRNA) for Nrf2 (siNrf2), ERK (siERK), and control siRNA (siCON) were purchased from Santa Cruz Biotechnology (Santa Cruz, CA, USA). Peroxidase-labeled goat anti-rabbit immunoglobulin was purchased from KOMA Biotechnology (Seoul, Korea). Cobalt protoporphyrin (CoPP) and zinc protoporphyrin (ZnPP) were obtained from Tocris Bioscience (Bristol, UK) and PD98059 was purchased from Calbiochem (San Diego, CA, USA). Dulbecco’s Modified Eagle’s Medium (DMEM), fetal bovine serum (FBS), and antibiotics were obtained from WelGENE Inc. (Daegu, Korea). Other chemicals were purchased from Sigma-Aldrich Chemical Co.

Jayasooriya R.G., Molagoda I.M., Dilshara M.G., Choi Y.H, & Kim G.Y. (2020). Glutamine Cooperatively Upregulates Lipopolysaccharide-Induced Nitric Oxide Production in BV2 Microglial Cells through the ERK and Nrf-2/HO-1 Signaling Pathway. Antioxidants, 9(6), 536.

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Modulation of Erk Signaling in SK-Hep1 Cells

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SK‐Hep1 cells were treated with TQ and different inhibitors including SB203580 (p38 MAP kinase inhibitor) and U0126 (Erk inhibitor). Erk expression of SK‐Hep1 cells was silenced by using si‐Erk (sc‐29 307; Santa Cruz Biotechnology, Dallas, TX) and negative control siRNA (4 390 843; Thermo Fisher Scientific, Shanghai, China). The siRNA was dissolved in RNase‐free solution and transfected into the cells using transfection reagent (sc‐29 528; Santa Cruz Biotechnology). After 36‐h culture, Erk expression was silenced in SK‐Hep1 cells.

Zhang B., Ting W., Gao J., Kang Z., Huang C, & Weng Y. (2021). Erk phosphorylation reduces the thymoquinone toxicity in human hepatocarcinoma. Environmental Toxicology, 36(10), 1990-1998.

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Knockdown of CDH12, CREB, and ERK

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For the knockdown experiments, cells were transfected with siCDH12 (sense: GCAAGCCACUUUACACCAUTT, antisense: AUGGUGUAAAGUGGCUUGCTT) or siNC (sense: UUCUCCGAACGUGUCACGUTT, antisense: ACGUGACACGUUCGGAGAATT)^{25 (link)}, siCREB (Santa Cruz Biotechnology, Cat# sc-29281), or siERK (Santa Cruz Biotechnology, Cat# sc-29307), using the Lipofectamine 2000 transfection reagent according to the manufacturer’s instructions.

Wang Y., Wang R., Liu X., Liu M., Sun L., Pan X., Hu H., Jiang B., Zou Y., Liu Q., Gong Y., Wang M, & Sun G. (2023). Chemotherapy-induced executioner caspase activation increases breast cancer malignancy through epigenetic de-repression of CDH12. Oncogenesis, 12(1), 34.

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