Cellasic onix2

Manufactured by Merck Group

Sourced in Germany

The CellASIC ONIX2 is a microfluidic cell culture platform designed for long-term live-cell imaging and automated cell culture experiments. It provides precise control over the cellular microenvironment, enabling researchers to conduct complex, time-lapse studies of cellular behavior and responses.

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Lab products found in correlation

Evos m7000 imaging system, by Thermo Fisher Scientific (2 mentions) Eclipse ts100, by Nikon (2 mentions) Eclipse ti e microscope, by National Instruments (2 mentions) Micromanager v 1, by National Instruments (2 mentions) Orca fusion bt, by Hamamatsu Photonics (1 mentions) Allegra x 22r centrifuge, by Beckman Coulter (1 mentions) Eclipse ti e microscope, by Nikon (1 mentions) Dmi8 inverted microscope, by Leica (1 mentions) Metamorph, by Universal Imaging (1 mentions) Emodin, by Tokyo Chemical Industry (1 mentions)

Spelling variants of this product

CellASIC ONIX2 Microfluidic System (11 citations) CellASIC (9 citations) CellASIC ONIX2 Manifold XT (2 citations) CellASIC ONIX2 system (2 citations)

9 protocols using cellasic onix2

Microfluidic Live-Cell Imaging Protocol

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Cells were cultured in the appropriate medium for 24 h and then diluted to an appropriate OD₆₀₀ such that after 12–16 h of growth the cell density was between OD₆₀₀ 0.05–0.1 the next day. This served two purposes: to achieve the appropriate cell-loading density and to limit medium acidification in the starter culture. Cells were loaded into a CellASIC ONIX plate for haploid yeast cells (Y04) and the appropriate medium was flowed over the cells (21 kPa) using a CellASIC ONIX2 microfluidic system (Millipore) for 1,000 min.
For the data presented in Fig. 1d, a custom microfluidic platform⁹ was used to image v-SEP and mCherry fluorescence every 5 min for ~1,000 min.

Okreglak V., Ling R., Ingaramo M., Thayer N.H., Millett-Sikking A, & Gottschling D.E. (2023). Cell cycle-linked vacuolar pH dynamics regulate amino acid homeostasis and cell growth. Nature Metabolism, 5(10), 1803-1819.

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Visualizing Golgi Disruption with BFA

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BFA (Sigma, #B7651) was dissolved in DMSO to prepare a 50 mM stock solution. Aliquots of the stock solution were added to the culture media to a final concentration of 200 μM (0.4% DMSO) for the experiments. The same amount of DMSO alone was used as a vehicle control. The BFA/DMSO-treated cells were observed using an inverted epifluorescence microscope (IX83, Evident/Olympus) equipped with a 100× objective (UPlanXApo) and a sCMOS camera (ORCA-FusionBT, Hamamatsu Photonics) (Figure 5A, B, and E–G) or SCLIM (Figure 5C and D). Perfusion experiments (Figure 5E–G) were performed using a microfluidic perfusion chamber system (CellASIC ONIX2, Millipore) with a flow pressure of 2 psi at 20°C according to the manufacturer’s protocol.

Tojima T., Suda Y., Jin N., Kurokawa K, & Nakano A. (2024). Spatiotemporal dissection of the Golgi apparatus and the ER-Golgi intermediate compartment in budding yeast. eLife, 13, e92900.

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Microfluidic Cell Chip Analysis Platform

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The microfluidic cell chip culture analysis platform CellASIC ONIX2 was purchased from Merck & Co., Inc. (Darmstadt, Germany); the EVOS M7000 imaging system was purchased from Invitrogen Life Technology Co., Ltd. (Carlsbad, CA, USA); the research-grade fluorescence inverted microscope ECLIPSE TS100 was purchased from Nikon Corporation (Nikon, Japan); the Allegra x-22R Centrifuge was purchased from Beckman coulter, Inc. (Brea, CA, USA); the MCO-18AIC CO₂ Incubator was purchased from Panasonic Corporation (Tokyo, Japan); and the SpectraMax I3X Enzyme marker was purchased from Molecular Devices Instruments Ltd. (San Jose, CA, USA).

Chen D., Yin J., Huo J., Sun J., Huang J., Li T., Sun C., Yang Z, & Qin W. (2022). Optimization and Application of A Bionic System of Dynamic Co-Culture with Hepatocytes and Renal Cells Based on Microfluidic Chip Technique in Evaluating Materials of Health Food. Nutrients, 14(22), 4728.

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Microfluidic Imaging of Caulobacter Colonization

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CB15 (holdfast⁺) cells were grown to the exponential phase in PYE medium and then loaded into a microfluidic plate (B04A-03; Merck Millipore). Medium flow and temperature were monitored with a CellASIC ONIX2 microfluidic system (Merck Millipore). The chamber was heated to 30°C, and the flow pressure was set at 2 lb/in². When the chamber was not attached to the system, it was incubated at 200 rpm and 30°C on a shaker in an airtight bag.
The growth chamber itself was constructed to trap cells via the use of a loading-pressure-dependent flexible ceiling. However, our intention was to follow Caulobacter surface colonization without the limitations imposed by the restrictions of the chamber. Therefore, we imaged cells in the broader and wider medium channels that lead into the growth chamber. Under those conditions, Caulobacter swarmer cells can swim along the channels and have space to settle on surfaces without getting trapped by the ceiling. After surface colonization, the channels were regularly flushed with diluted rich medium applied at 2 lb/in² to keep the channel above the biofilm mostly cell free.

Heinrich K., Leslie D.J., Morlock M., Bertilsson S, & Jonas K. (2019). Molecular Basis and Ecological Relevance of Caulobacter Cell Filamentation in Freshwater Habitats. mBio, 10(4), e01557-19.

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Time-lapse fluorescence microscopy of heat shock

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Fluorescence microscopy was performed using a Nikon Eclipse Ti-E microscope with Micro-Manager software. For time-lapse experiments, cells were loaded in commercial microfluidic chips (CellASIC ONIX2, Merck Millipore) at 30 °C as previously described [4] . Heat shock was induced thanks to the chip's temperature controller by increasing the temperature to 42 °C for 30 min, before returning it to 30 °C, or by shifting cultures for 30 min in a shaking incubator (Kühner shaker, LT W Lab-Therm) pre-heated at 42 °C (the two heat shock methods proved to be equivalent). Images were recorded every 10 min. Quantifications were carried out as described in the corresponding figure legends.

Cereghetti G., Wilson-Zbinden C., Kissling V.M., Diether M., Arm A., Yoo H., Piazza I., Saad S., Picotti P., Drummond D.A., Sauer U., Dechant R., & Peter M. (2021). Reversible amyloids of pyruvate kinase couple cell metabolism and stress granule disassembly.

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Visualizing C. botulinum Spore Germination

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C. botulinum ATCC3502 spores were prepared as described^{55 (link)} and fixed into a microfluidic plate using CellASIC ONIX2 microfluidic system (Merck Millipore) according to the manufacturer's instructions in an anaerobic workstation. TPGY medium was perfused into the microfluidic chamber with the pressure of 13.8 kPa for 6 h to enable sufficient spore germination. After 20 µM of CBO1751 or control DB was perfused into the microfluidic plate to replace TPGY medium, phase-contrast images of newly germinated cells were taken every 20 s over 60 min using a Leica DMi8 inverted microscope with a 100-fold oil‐immersion lens (Leica Microsystems, Wetzlar, Germany). The images were processed using Metamorph (Universal Imaging, Bedford Hills, NY, USA).

Zhang Z., Lahti M., Douillard F.P., Korkeala H, & Lindström M. (2020). Phage lysin that specifically eliminates Clostridium botulinum Group I cells. Scientific Reports, 10, 21571.

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Time-lapse Fluorescence Microscopy of Heat Shock

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Fluorescence microscopy was performed using a Nikon Eclipse Ti-E microscope with MicroManager V 1.4 and NIS-Elements Advanced Research V 5.02 software. For time-lapse experiments, cells were loaded in microfluidic plates (CellASIC ONIX2, Merck Millipore) at 30 °C [4 (link)]. Heat shock was induced with the microfluidic plate temperature controller by increasing the temperature to 42 °C for 30 min, before returning it to 30 °C, or by shifting cultures for 30 min in a shaking incubator (Kühner, LT W Lab-Therm) pre-heated at 42 °C. Images were recorded every 10 min, and quantified as described in the figure legends.

Cereghetti G., Wilson-Zbinden C., Kissling V.M., Diether M., Arm A., Yoo H., Piazza I., Saad S., Picotti P., Drummond D.A., Sauer U., Dechant R, & Peter M. (2021). Reversible amyloids of pyruvate kinase couple cell metabolism and stress granule disassembly. Nature cell biology, 23(10), 1085-1094.

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Time-lapse Fluorescence Microscopy of Heat Shock

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Emodin's Effects on Endothelial Cells

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In this study, the main materials include: Emodin (Tokyo chemical industry Co., Ltd., Tokyo, Japan);Endothelial Cell Medium (ECM) (ScienCell biotechnology company, San Diego, CA, USA); Dulbecco modified Eagle’s minimal essential medium (DMEM) (Gibco Life Technologies, Grand Island, NY, USA); Trypsin-EDTA Solution (0.25%) and rat tail type I collagen (Beijing Solarbio Science & Technology, Co. Ltd., Beijing, China); Fetal bovine serum (GEMINI company, Woodland, CA, USA); Gelatine (Shanghai Aladdin Biochemical Technology Co., Ltd., Shanghai, China); Live/dead cell viability/toxicity test kit (Nanjing KeyGen Biotech. Co., Ltd., Jiangsu, China); Cell count kit (CCK-8) (Beyotime Biotechnology Co., Ltd., Shanghai, China); Human lactate dehydrogenase (LDH), aspartate aminotransferase (AST) and urea nitrogen (BUN) kits (Nanjing Jiancheng Bioengineering Research Institute Co., Ltd., Nanjing, China). The main instruments include: CellASIC ONIX2 (Merck & Co Inc, Darmstadt, Germany); EVOS M7000 Imaging system (Invitrogen Life Technology Co., Ltd., Carlsbad, CA, USA); ECLIPSE TS100 (Nikon Corporation, Tokyo, Japan); Thermo Scientific LegendMicro (Thermo Scientific Co., Ltd., Waltham, MA, USA); SpectraMax I3X Enzyme marker (Molecular Devices Instruments Ltd., San Jose, CA, USA).

Chen D., Yin J., Yang Z., Qin W., Huo J., Huang J., Sun J, & Piao W. (2022). Construction and Application of Hepatocyte Model Based on Microfluidic Chip Technique in Evaluating Emodin. Nutrients, 14(13), 2768.

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