Log In Sign up
The largest database of trusted experimental protocols

Anti actb mouse monoclonal antibody

Manufactured by GenScript
Visit Supplier

The Anti-ACTB mouse monoclonal antibody is a laboratory reagent used for the detection and quantification of the ACTB (β-actin) protein in various biological samples. This antibody specifically binds to the ACTB protein, which is a widely expressed and highly conserved cytoskeletal protein. The core function of this antibody is to serve as a tool for researchers to study the expression and localization of ACTB in their experiments.

Automatically generated - may contain errors

Lab products found in correlation

Blue loading buffer pack, by Cell Signaling Technology (2 mentions) Novex 16 tris glycine mini gels, by Thermo Fisher Scientific (2 mentions) Anti lin28b antibody, by Cell Signaling Technology (2 mentions) Thermo scientific myecl imager, by Thermo Fisher Scientific (2 mentions)

2 protocols using anti actb mouse monoclonal antibody

1

LIN28B Western Blot Protocol

Check if the same lab product or an alternative is used in the 5 most similar protocols
Whole-cell lysates for Western blots were extracted with Blue Loading Buffer Pack (Cell Signaling Technology, 7722S). Protein lysates were loaded into Novex 16% Tris-Glycine Mini Gels (Thermo Fisher Scientific, XP99165BOX) and separated by gel electrophoresis at 125V for 4 hours. LIN28B and β-actin were detected using an anti-LIN28B antibody (Cell Signaling Technology, #4196) and an anti-ACTB mouse monoclonal antibody (GenScript, A00702), respectively. More details about the antibodies can be found in the Life Sciences Reporting Summary. The Western blot was imaged with Thermo Scientific myECL Imager (Thermo Scientific, 62236).
Jang H.S., Shah N.M., Du A.Y., Dailey Z.Z., Pehrsson E.C., Godoy P.M., Zhang D., Li D., Xing X., Kim S., O’Donnell D., Gordon J.I, & Wang T. (2019). Transposable elements drive widespread expression of oncogenes in human cancers. Nature genetics, 51(4), 611-617.
+ Open protocol
+ Expand
2

LIN28B Western Blot Protocol

Check if the same lab product or an alternative is used in the 5 most similar protocols
Whole-cell lysates for Western blots were extracted with Blue Loading Buffer Pack (Cell Signaling Technology, 7722S). Protein lysates were loaded into Novex 16% Tris-Glycine Mini Gels (Thermo Fisher Scientific, XP99165BOX) and separated by gel electrophoresis at 125V for 4 hours. LIN28B and β-actin were detected using an anti-LIN28B antibody (Cell Signaling Technology, #4196) and an anti-ACTB mouse monoclonal antibody (GenScript, A00702), respectively. More details about the antibodies can be found in the Life Sciences Reporting Summary. The Western blot was imaged with Thermo Scientific myECL Imager (Thermo Scientific, 62236).
Jang H.S., Shah N.M., Du A.Y., Dailey Z.Z., Pehrsson E.C., Godoy P.M., Zhang D., Li D., Xing X., Kim S., O’Donnell D., Gordon J.I, & Wang T. (2019). Transposable elements drive widespread expression of oncogenes in human cancers. Nature genetics, 51(4), 611-617.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!