On days 1, 3, and 7 after surgery, blood was obtained via retroorbital bleeding and allowed to clot at RT. The levels of inflammation factors in sera, namely, TNF-α, IL-6, and IL-10, were measured using the Mouse TNF alpha ELISA kit (Abcam, USA), Mouse IL-6 ELISA kit (Abcam, USA), and Mouse IL-10 ELISA kit (Abcam, USA) as per the manufacturer’s instructions.
Mouse il 10 elisa kit
Manufactured by Abcam
Sourced in United States, United Kingdom
The Mouse IL-10 ELISA Kit is a quantitative sandwich enzyme-linked immunosorbent assay (ELISA) for the measurement of mouse interleukin-10 (IL-10) levels in biological samples.
Automatically generated - may contain errors
Lab products found in correlation
Mouse il 6 elisa kit, by Abcam (7 mentions)
Mouse tnf alpha elisa kit, by Abcam (6 mentions)
Mouse il 1 beta elisa kit, by Abcam (3 mentions)
Mouse il 1β elisa kit, by Abcam (3 mentions)
Mouse tnf α elisa kit, by Abcam (2 mentions)
Pierce bca protein assay kit, by Thermo Fisher Scientific (2 mentions)
Attune cytpix, by Thermo Fisher Scientific (1 mentions)
Mouse ifn γ elisa kit, by Abcam (1 mentions)
Mouse il 17a elisa kit, by Abcam (1 mentions)
Mitotracker orange cmtmros, by Thermo Fisher Scientific (1 mentions)
Mitotracker green fm, by Thermo Fisher Scientific (1 mentions)
Dimethyl sulfoxide (dmso), by Solarbio (1 mentions)
Escherichia coli derived lps, by Merck Group (1 mentions)
Facscan, by BD (1 mentions)
Enspire multimode plate reader, by PerkinElmer (1 mentions)
Procollagen type 1 c peptide pip eia kit, by Takara Bio (1 mentions)
Rat il 10 elisa kit, by Abcam (1 mentions)
Rat il 6 elisa kit, by Abcam (1 mentions)
Rat il 1 beta elisa kit, by Abcam (1 mentions)
Rat tnf alpha elisa kit, by Abcam (1 mentions)
18 protocols using mouse il 10 elisa kit
1
Assaying Inflammatory Cytokines Post-Surgery
2
Inflammatory Response in Bone Defect Mice
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To evaluate the inflammatory response in bone defect mouse models, tail venous blood of mice was extracted on the 4th day after treatment. The supernatant was collected via centrifugation (1,000 rpm) to detect IL-1β, IL-4, and IL-10 levels by ELISA. Mouse IL-1 beta ELISA Kit (ab197742), Mouse TNF alpha ELISA Kit (ab100747), and Mouse IL-10 ELISA Kit (ab255729) were purchased from Abcam. For immune function detection, the blood samples of mice were collected to separate peripheral blood mononuclear cells (PBMC) by density gradient centrifugation, and PBMC concentration was adjusted to 1 × 106/mL with binding buffer solution. A PBMC suspension (100 μL) was transferred to a 5 mL PE tube, and PE Anti-CD4 antibody [EPR20122] (ab252151) and Alexa Fluor® 647 anti-CD8 alpha antibody [EPR21769] (ab237365) were used to separately label CD4 and CD8. Then, 5 μL of annexin V-FITC and 5 μL of propidium iodide were placed to the above mixture solution. After mixing and incubating in the dark for 15 min, flow cytometry (Attune CytPix, Thermo Fisher Scientific) was used to detect lymphocyte apoptosis.
3
Chitooligosaccharides Modulate Inflammatory Response
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According to previous reports, RAW264.7 cells were seeded in 96-well plates at a density of 1 × 104 cells/well and incubated in the above medium with 100 μM different DP of COS (chitobiose, chitotriose, chitotetraose, chitopentaose, chitohexaose, chitoheptaose, and chitooctaose) for 24 h and stimulated with LPS (200 ng/mL, 6 h). RAW264.7 cells were washed 3 times with cold PBS and centrifuged at 1,000 g for 10 min at 4°C. The cell pellet thus obtained was resuspended in 0.5 mL Tris buffer (20 mM, pH 7.5, 1 μg/mL chymostatin, 2 μg/mL of pepstatin A, 100 μM phenylmethylsulfonyl fluoride, and 5 μg/mL aprotinin). The cells were lysed by two freeze-thaw cycles [20 (link)]. The supernatants were collected via centrifugation at 15,000 g for 10 min at 4°C. The inflammatory production was measured by using the kits (mouse IL-1β ELISA Kit, ab100704; mouse IL-10 ELISA Kit, ab108870; mouse IL-17A ELISA Kit, ab100702; and mouse IFN-γ ELISA Kit, ab100689; Abcam, USA).
4
Mitochondrial Dynamics and Galectin-3 Inhibition
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Mitochondrion-selective probes, including MitoTracker® Green FM (Invitrogen, Carlsbad, CA, USA) and MitoTracker® Orange CMTMRos (Invitrogen, Carlsbad, CA, USA), were used to label the mitochondria of the NK cells. TD139 (TargetMol, Wellesley Hills, MA, USA) was dissolved in dimethyl sulfoxide (DMSO) (Solarbio, Beijing, China) and used for the inhibition of Galectin-3 in vivo (15 μg/g body weight, once per 24 h for three times). A Galectin-3 mouse ELISA Kit (Invitrogen, Carlsbad, CA, USA) was used to determine the serum and liver homogenate levels of Galectin-3. A mouse IL-10 ELISA kit (Abcam, Cambridge, MA, USA) was used to determine the serum and liver homogenate levels of IL-10.
5
Hyp-PDT and GSH Effects on imDCs
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LLC cells exposed to high dose of hyp-PDT treatment with or without GSH were co-incubated with imDCs (day 6) at a ratio of 1:5 (imDCs: LLCs) for 24 h. ImDCs (day 6) were stimulated with 100 ng/ml of Escherichia coli-derived LPS (Sigma-Aldrich, San Luis, USA) for 24 h, and were regarded as positive controls for DC maturation. The cells were detached, washed and stained with the following antibodies: FITC-anti-CD86, anti-CD80 and anti-CD40 according to the manufacturer's instructions. FITC-conjugated armenian hamster IgG and FITC-conjugated rat IgG2a antibodies were used as isotype controls. The DC cells were analyzed using a FACScan (BD Bioscience) flow cytometer. The DCs were gated according to the forward (FSC) and side scatter (SSC) properties and 50,000 viable DCs were acquired for each experiment. The co-culture supernatant derived from these imDCs and LLCs co-incubation experiments was collected for further analysis. NO in the supernatant was quantified by Griess assay 21 (link). Immunoreactive levels of mouse IL-10 in the media was measured by using mouse IL10 elisa kit (Abcam, Cambridge, UK). The samples were prepared according to the manufacturers' instructions and analyzed on EnSpire® Multimode Plate Reader (PerkinElmer, Massachusetts, USA).
6
Quantification of Skin Biochemical Markers
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Skin tissues in radioimmunoassay precipitation buffer were homogenized by using a Taco™ Prep bead beater (GeneReach Biotechnology; Taishung, Taiwan) and a KS-750 ultrasonic disruptor (Madell Technology; Ontario, CA, USA), centrifuged at 15,000× g for 10 min, and then supernatants were collected as skin homogenates. According to the manufacturer’s instructions, levels of type I collagen, hyaluronan, interleukin (IL)-1β, and IL-10 in skin homogenates were measured using procollagen type I C-peptide (PIP) EIA kit (Takara; Shiga, Japan), mouse hyaluronic acid ELISA Kit (Mybiosource; San Diego, CA, USA), mouse IL-1β ELISA Kit (Abcam), and Mouse IL-10 ELISA Kit (Abcam), respectively. In case of hyaluronan measurement, trichloroacetic acid was added and then centrifuged to remove proteins from the skin homogenates.
7
Quantifying Cytokine Levels in Cell Culture
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The concentrations of interleukin-6 (IL-6), interleukin-10 (IL-10), and tumor necrosis factor-α (TNF-α) in cell culture supernatant and serum were detected in the 96-well plate using the commercialized ELISA kits, including Mouse IL-6 ELISA Kit (ab222503, Abcam), Mouse IL-10 ELISA Kit (ab255729, Abcam), Mouse TNF alpha ELISA Kit (ab208348, Abcam). The ELISA procedure was conducted as per the manufacturer’s protocol (Abcam, USA).
8
Cytokine Profiling in Mouse Model
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Mouse IL-6 ELISA kit (ab100712), mouse IL-10 ELISA kit (ab108870), and mouse TNF-α ELISA kit (ab208348) were purchased from Abcam (Boston, MA, USA). Serum levels of cytokines were measured by using the corresponding kits after 3-day model establishment.
9
Cytokine Profiling in Osteoarthritis
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The synovial fluid samples of OA rats and the supernatant samples of RAW264.7 cells were collected. Cytokine levels were measured using the following ELISA kits, according to the manufacturer's protocol (All from Abcam, USA): Mouse IL-6 ELISA Kit, Mouse IL-1 beta ELISA Kit, Mouse TNF alpha ELISA Kit, Mouse IL-10 ELISA Kit, Rat IL-6 ELISA Kit, Rat IL-1 beta ELISA Kit, Rat TNF alpha ELISA Kit, and Rat IL-10 ELISA Kit. The results were tested using a multifunctional enzyme marker.
10
Cytokine Production Profiling
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The production of IL-6, TNF-α, IL-1β, IL-10, IL-12, and IL-13 were detected by using Mouse IL-6 ELISA Kit (ab222503; Abcam), Mouse TNF alpha ELISA Kit (ab208348, Abcam), Mouse IL-1 beta ELISA Kit (ab197742, Abcam), Mouse IL-10 ELISA Kit (ab255729, Abcam), Mouse IL-12(p70) ELISA Kit (EK0422; ScienCell™), Mouse IL-13 ELISA Kit (BMS6015, invitrogen), respectively, according to the manufacturer’s instruction.
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