Anti-Flag (A00187–200), anti-HA (A01244–100), anti-Actin (A00730–100), and protein G resin (L00209) were purchased from GenScript. Anti-NLRP3 (AG-20B-0014-C100) was purchased from Adipogen. Anti-GSDMD (ab209845) and anti-NEK7 (ab133514) were purchased from Abcam. Anti-ASC (67824) was purchased from CST. Anti-mouse caspase-1 was a kind gift from Dr Gabriel Núñez (University of Michigan Medical School). Ultra-pure LPS (tlrl-pb5lps), Nano-SiO2 (tlrl-sio), and poly(dA:dT)/lyovec (tlrl-patc) were purchased from InvivoGen. Nigericin (481990), doxycycline hydrochloride (10592–13–9), and EDTA-free protease inhibitor cocktail (11873580001) were purchased from Sigma. Salmonella strain SL1344 was originally from Dr Denise Monack (Stanford University). The constructs for NLRP3-Flag, NLRP3-SFP, and NEK7-HA have been previously described (17 (link)).
Anti nek7 ab133514
Manufactured by Abcam
Sourced in United Kingdom
Anti-NEK7 (ab133514) is a primary antibody that recognizes the NEK7 protein. NEK7 is a serine/threonine-protein kinase involved in mitotic progression. This antibody can be used in various research applications to detect and study the NEK7 protein.
Automatically generated - may contain errors
Lab products found in correlation
Anti gsdmd ab209845, by Abcam (2 mentions)
Doxycycline hydrochloride, by Merck Group (1 mentions)
Anti flag, by GenScript (1 mentions)
Anti nlrp3 ag 20b 0014 c100, by Adipogen (1 mentions)
Protein g resin, by GenScript (1 mentions)
Edta free protease inhibitor cocktail, by Merck Group (1 mentions)
Anti mouse caspase 1 ag 20b 0042, by Adipogen (1 mentions)
Anti β actin 66009 1 ig, by Proteintech (1 mentions)
Anti nlrp3 ag 20b 0014, by Adipogen (1 mentions)
Mitotracker, by Beyotime (1 mentions)
Mitosox, by Thermo Fisher Scientific (1 mentions)
Protein a g agarose, by Beyotime (1 mentions)
2 protocols using anti nek7 ab133514
1
NLRP3 Inflammasome Activation Assay
2
Investigating NLRP3 Inflammasome Activation
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Lipopolysaccharides (LPS, #L2880), adenosine triphosphate (ATP, #A3377), and aluminum potassium sulfate dodecahydrate (Alum, #237086) were purchased from Sigma (St. Louis, MO, USA). COS was bought from Abphyto Biotech Co., Ltd. (Chengdu, China). MitoSOX (#M36008) was obtained from Invitrogen (Carlsbad, CA, USA). MitoTracker (#C1049B) and protein A + G agarose (#P2012) were supplied by Beyotime (Shanghai, China). Anti-NLRP3 (#AG-20B-0014) and anti-mouse caspase-1 (#AG-20B-0042) antibodies were purchased from Adipogen (San Diego, CA, USA). Anti-NEK7 (#ab133514) and anti-GSDMD (#ab209845) antibodies were obtained from Abcam (Cambridge, UK). Anti-mouse IL-1β (#AF-401-NA) antibody was from R&D Systems (Minneapolis, MN, USA). Anti-Flag (#20543-1-AP), anti-HA (#66006-2-Ig), and anti-β-actin (#66009-1-Ig) antibodies were purchased from Proteintech (Rosemont, IL, USA).
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