Ammonium acetate
Ammonium acetate is a chemical compound commonly used in laboratory settings. It serves as a buffering agent, maintaining a specific pH level in various analytical and experimental procedures. Ammonium acetate is a white, crystalline solid that is soluble in water and other polar solvents.
Lab products found in correlation
16 protocols using ammonium acetate
Quantitative Proteomics Analysis Protocol
Quantitative Analysis of Bioactive Compounds
UHPLC-QTOF-MS for Metabolite Profiling
TripleTOF 5600 mass spectrometry (AB Sciex) was used for its ability to acquire MS/MS spectra on an information-dependent basis (IDA) during LC/MS experiments. In this mode, the acquisition software (Analyst TF 1.7, AB Sciex) continuously evaluates the full scan survey MS data as it collects and triggers the acquisition of MS/MS spectra depending on preselected criteria. In each cycle, the most intensive 12 precursor ions with intensity above 100 were chosen for MS/MS at a collision energy (CE) of 30 eV. In the detection process, standard products and blank controls were strictly used for quality control, and data quality control was also carried out. The UHPLC-QTOF-MS analysis was performed at Biomarker Technologies Corporation, Beijing, China (
Ergosterol Purification and Characterization
PFAS Quantification Protocol
BPA Quantification by LC-MS/MS
Stock solutions of BPA and 13 C12-BPA were prepared at a concentration of 1 mg/mL in methanol. All stock solutions were stored at -20°C. Calibration standards, ranging from 0.20 to 25 μg/L, were prepared from the stock solution through serial dilution by using a methanol-water mixture (50/50, v/v); isotope stock solutions ( 13 C12-BPA, 100 μg/L) were also prepared by using the same methanol-water mixture.
UHPLC-MS/MS Analysis of Metabolites
MS/MS data acquisition and analysis were conducted using the Analyst TF v1.7 (AB Sciex, MA, United States) according to a preset standard. In each cycle, a collision energy (CE) of 30 eV and cycle time of 0.56 s was used to select the densest 12 precursor ions with an intensity >100 for MS/MS. The following were set as the electrospray ionization (ESI) source conditions: curtain gas (35 psi), gas 1 (60 psi), gas 2 (60 psi), ion spray voltage floating (5,000 V in the positive mode and −4,000 V in the negative mode), declustering potential (60 V), and source temperature (600°C) (19 (link)).
Quantification of Oxidative Stress Markers
Metabolomic Analysis of Botanical Samples
Major instruments used in this study included ultra-high performance liquid chromatography (UHPLC) (Agilent Technologies, Santa Clara, CA, USA) coupled with Q Exactive Focus mass spectrometer (MS) (Thermo Fisher Scientific, Waltham, MA, USA), centrifuge, scales, grinding mill, water purification, ultrasound instrument, and column (ACQUITY UPLC HSS T3 1.7 μm, 2.1 × 100 mm).
Dexamethasone Injection Quantification
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!