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Erk 4696

Manufactured by Cell Signaling Technology
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ERK (4696) is a recombinant protein that represents the extracellular signal-regulated kinase (ERK) 1 and ERK2 enzymes. ERK 1/2 are serine/threonine protein kinases that play a key role in the regulation of cell proliferation, differentiation, and survival.

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Lab products found in correlation

Phospho erk 9101, by Cell Signaling Technology (1 mentions) α tubulin, by Merck Group (1 mentions) Protease inhibitor cocktail, by Roche (1 mentions) β actin, by Merck Group (1 mentions)

2 protocols using erk 4696

1

Immunoblotting Protocol for Protein Analysis

Cited 1 time
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For immunoblot analysis, cells were washed twice with PBS and lysed in lysis buffer (1% NP-40, 120 mM NaCl, 40 mM Tris-HCl (pH7.4), 1.5 mM sodium orthovanadate, 50 mM sodium fluoride, 10 mM sodium pyrophosphate, and protease inhibitor cocktail (Roche)), as described in (Cai et al. 2020 (link); Lee et al. 2020 (link)). Primary antibodies against phospho-Akt (9272), Akt (4056), phospho-ERK (9101), ERK (4696) (Cell Signaling), α-tubulin (T5169; Sigma), and horseradish peroxidase-conjugated secondary antibodies were used. IPMK antibody was generated with a peptide targeting mouse IPMK amino acids 295-311 (SKAYSRHRKLYAKKHQS) from Covance.
Ahn H., Roh J.S., Lee S., Beon J., Lee B., Sohn D.H, & Kim S. (2021). Myeloid IPMK promotes the resolution of serum transfer-induced arthritis in mice. Animal Cells and Systems, 25(4), 219-226.
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2

Probing Protein Signaling Pathways

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Western blotting was performed as described previously.61 (link) Cells were grown in 100 mm dishes to about 50% confluence and treated with vehicle (control) and CTN06 (0.5 and 2 μM) for 12 h. Proteins were detected by the following antibodies: β-actin (Sigma-Aldrich, A1978), Btk (Santa Cruz Inc., sc-1107), pBtk (pY233, Abcam, ab68217), Etk (Santa Cruz Inc., sc8874), pEtk (pY40, 3211S), Src (2109), pSrc (pY416, 6943), PLCγ2 (3872), pPLCγ2 (pY759, 6943), Stat3 (12640), pStat3 (pY705, 4113), ERK (4696) and pERK (4695) were obtained from Cell Signaling Inc. (Beverly, MA, USA). For pBtk and pEtk, cells were pre-treated with 100 μM pervanadate for 15 min before harvest.
Guo W., Liu R., Bhardwaj G., Yang J.C., Changou C., Ma A.H., Mazloom A., Chintapalli S., Xiao K., Xiao W., Kumaresan P., Sanchez E., Yeh C.T., Evans C.P., Patterson R., Lam K.S, & Kung H.J. (2014). Targeting Btk/Etk of prostate cancer cells by a novel dual inhibitor. Cell Death & Disease, 5(9), e1409-.
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