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26 protocols using arium 611 uv

1

Solvent Extraction and Purification

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All solvents required for extraction and isolation were purchased from VWR International (Vienna, Austria), and ethyl acetate was distilled before use. Solvents for analytical experiments had at least pro analysis (p.a.) quality and were obtained from Merck (Darmstadt, Germany). Deuterated solvents were supplied by Euriso-Top (Saint-Aubin Cedex, France). Ultrapure water was produced by a Sartorius arium® 611 UV (Göttingen, Germany) purification system. Silica gel 40–63 μm and pre-packed cartridges for flash chromatography were purchased from Merck (Darmstadt, Germany) and Büchi (Flawil, Switzerland), respectively.
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2

Carotenoid and Porphyrin Chemical Synthesis

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All-trans-β-carotene (Type II synthetic, purity >95%), lutein from marigold, β-cryptoxanthin (purity >97%), zeaxanthin (purity >95%), lycopene from tomato (purity >90%), butylhydroxytoluene (BHT, purity ≥99%) and triethylamine (TEA, purity ≥99%) were obtained from Sigma-Aldrich (Shnelldorf, Germany). High-performance liquid chromatography (HPLC) organic solvents were of analytical grade: methanol (MeOH), absolute ethanol (EtOH), chloroform and hexane were from Carlo Erba (Val-de-Reuil, France), methyl-tert-butyl-ether (MTBE) was from Fisher Scientific (Loughborough, UK), petroleum ether (PE) was from VWR Prolabo (Fontenay-sous-Bois, France). Ultrapure water was obtained from a purified water system Arium® 611UV from Sartorius (Göttingen, Germany) with a resistivity of 18.2 MΩ*cm. Sodium chloride (NaCl, purity >99%) and potassium hydroxide (KOH, purity >99%) were obtained from VWR Prolabo (Fontenay-sous-Bois, France), and activated magnesium silicate Florisil® at 100–200 mesh (Sigma-Aldrich, Shnelldorf, Germany). Liquid Nitrogen was from Air Liquide (Nancy, France). The synthesis of 5-(4-carboxyphenyl)-10,15,20-triphenyl porphyrin (P1COOH) has been described elsewhere [25 (link)].
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3

Analytical Reagents and Water Purification

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Solvents for analytical experiments in analytical quality (p.a.) were obtained from Merck (Darmstadt, Germany). Ultrapure water was produced by a Sartorius arium® 611 UV (Göttingen, Germany) purification system.
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4

Synthesis and Characterization of Optoelectronic Materials

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Triethoxysilane (TES) was purchased from TCI chemicals (Tokyo, Japan). 1-Decene was purchased from Sigma-Aldrich (Saint Louis, MO, USA) and was used as received. Electronic grade hydrofluoric acid (49% aqueous solution, Kanto Chemical, Tokyo, Japan), Toluene (High Performance Liquid Chromatography, HPLC, grade), dicholrobenzene, ethanol, and methanol were purchased from Wako chemical (Tokyo, Japan). Colloidal ink of zinc oxide (ZnO, Sigma-Aldrich), 4,4′-bis(carbazole-9-yl)biphenyl (CBP, 99.9% trace metals basis, Sigma-Aldrich), and molybdenum (VI) oxide (MoO3, 99.97% trace metals basis, Sigma-Aldrich) were used as received. Water was purified and deionized using a Sartorius (Arium 611 UV, Sartorius AG, Göttingen, Germany) water purification system.
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5

Extraction and Isolation Procedures

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All solvents required for extraction and isolation were purchased from VWR International (Vienna, Austria) and petroleum ether (PE), dichloromethane, acetone, and ethyl acetate (EtOAc) were distilled before use. Solvents for analytical experiments had pro analysis (p.a.) quality at least and were obtained from Merck (Darmstadt, Germany). Deuterated solvents were supplied by Euriso-Top (Saint-Aubin, France). Ultrapure water was produced by a Sartorius arium® 611 UV (Göttingen, Germany) purification system. Silica gel 40–63 μm and pre-packed cartridges for flash chromatography were purchased from Merck (Darmstadt, Germany) and Büchi (Flawil, Switzerland), respectively.
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6

Analytical Techniques for Natural Dyes

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HPLC-quality water was
obtained from a purification system (R = 18.2 MΩ
cm; Arium 611UV, Sartorius, Göttingen, Germany). Methanol (MeOH,
LC-MS grade) was obtained from Biosolve (Valkenswaard, The Netherlands).
Crystal violet (CV; ≥90%), eosin Y (EY; 99%), riboflavin (RF;
≥98%), ammonium formate (AF; ≥99.0%), and formic acid
(FA; ≥95%) were purchased from Sigma-Aldrich (Zwijndrecht,
The Netherlands).
Fuchsin was a gift from the Cultural Heritage
Agency of The Netherlands (RCE, Amsterdam, The Netherlands). Annatto
seeds were purchased from De Peperbol (Amsterdam, The Netherlands),
and the Davitamon vitamin-B pills were purchased from Etos (RF concentration
2.8 mg per pill, Amsterdam, The Netherlands).
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7

Glutathione Redox Assay Protocol

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Ultra-pure water (<18 MΩ·cm, <5 μgL−1 TOC) was obtained from a purification system Arium 61316-RO plus Arium 611 UV (Sartorius, Goettingen, Germany). Nitroblue Tetrazolium, Xylenol Orange, Glutathione Reductase, Reduced Glutathione, Oxidized Glutathione, NADPH, and Ellman’s reagent and glucose determination commercial kit were provided by Sigma Aldrich (Buenos Aires, Argentina). The lactate determination commercial kit was obtained from Wiener Lab Group (Buenos Aires, Argentina) and Isoflurane from Piramal Healthcare (Morphet, UK). All other reagents were of analytical grade.
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8

Preparation of Standard Solutions

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Methanol had pro-analysis (p.a) quality and was purchased from Merck (Darmstadt, Germany). Formic acid and acetic acid were obtained from VWR International (Vienna, Austria). A Sartorius Arium® 611 UV (Sartorius, Göttingen, Germany) purification system was used for the production of ultrapure water.
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9

Extraction and Isolation Protocols

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All solvents which are required for extraction and isolation were purchased from VWR International (Vienna, Austria), and ethyl acetate was distilled before use. Solvents for analytical experiments had pro analysis (p.a.) quality at least and were obtained from Merck (Darmstadt, Germany). Deuterated solvents were supplied by Euriso‐Top (Saint‐Aubin Cedex, France). Ultrapure water was produced by a Sartorius arium® 611 UV (Göttingen, Germany) purification system. Silica gel 40–63 μm and pre‐packed cartridges for flash chromatography were purchased from Merck (Darmstadt, Germany) and Büchi (Flawil, Switzerland), respectively.
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10

Preparation of Amidated Peptide Samples

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All synthetic model peptides were obtained
from GL Biochem and used as received with no further purification.
HPLC-grade methanol and formic acid were supplied by Merck (Darmstadt,
Germany). The water was ultrapure grade (Arium 611 UV, Sartorius AG,
Goettingen, Germany). Stock solutions of peptides were prepared by
dissolving solid material in a 1:1 (v/v) mixture of methanol and water
to a concentration of 10–2 M. Peptide samples at
micromolar concentration level were prepared by diluting stock solutions
with 1:1 methanol:water containing 1% formic acid. All of the model
peptides are C-terminally amidated except for YIHPFHL-OH.
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