ES cells were grown on a layer of feeder cells in DMEM-ES (Sigma D6546) medium supplemented with 15% FCS, 1 mM sodium pyruvate, 1 mM glutamine, 100 units per ml penicillin and 100 μg per ml streptomycin, 0.15 mM MTG, 25 mM HEPES buffer, 1× non-essential amino acids (Sigma) and 103 units per ml leukaemia inhibitory factor (ESGRO mLIF, Millipore ESG1107). Medium was changed every day. Before in vitro differentiation, the cells were grown without feeder cells for 2 passages and the medium used for the last passage was IMDM-ES (Sigma I3390).
Imdm es
Manufactured by Merck Group
IMDM-ES is a basal cell culture medium that provides a nutrient-rich environment for the growth and maintenance of cells. It contains a balanced formulation of amino acids, vitamins, salts, and other essential components to support cell proliferation and viability.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using imdm es
1
Murine Embryonic Stem Cell Culture
2
Expansion and Differentiation of ESCs
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ES cells were grown on a layer of feeder cells in DMEM-ES (Sigma D6546) medium supplemented with 15% FCS, 1 mM sodium pyruvate, 1 mM glutamine, 100 units per ml penicillin and 100 μg ml−1 streptomycin, 0.15 mM MTG, 25 mM HEPES buffer, 1 × non-essential amino acids (Sigma) and 103 units per ml LIF (ESGRO mLIF, Millipore ESG1107). Medium was changed every day. Before in vitro differentiation (IVD), the cells were grown without feeder cells for two passages and the medium used for the last passage was IMDM-ES (Sigma I3390).
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