Iron 3 sulfate hydrate

Manufactured by Thermo Fisher Scientific

Sourced in United Kingdom

Iron (III) sulfate hydrate is a chemical compound used in various laboratory applications. It is a yellow-green crystalline solid that is soluble in water. The main function of iron (III) sulfate hydrate is to serve as a source of ferric ions (Fe3+) in chemical reactions and analyses.

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3 protocols using iron 3 sulfate hydrate

Synthesis and Characterization of Functionalized Silica Nanoparticles

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The chemicals were reagent grade from commercial sources. Iron (III) sulfate hydrate, iron (II) sulfate heptahydrate (99+%), citric acid (99+%), tetraethoxysilane (99.9%), 3-(2-aminoethylamino)propylmethyldimethoxysilane (97%), N,N-dimethylformamide (99%), and succinic anhydride (99%) were purchased from Alfa Aesar, Ward Hill, MA, USA. The acetone (AppliChem GmbH, Darmstadt, Germany), ethanol absolute (Carlo Erba, Val de Reuil, France, reagent – USP), NH₄OH (aq) (Fluka, pa, 25%), and HCl 1 M (pa, Riedl-de-Haën) were used as received. The lipids POPC and DPPC were purchased from Avanti Polar Lipids Inc., Alabaster, AL, USA. The fluorophores 1,6-diphenyl-1,3,5-hexatriene (DPH), (1-(4-trimethylammoniumphenyl)-6-phenyl-1,3,5-hexatriene p-toluenesulfonate) (TMA-DPH) and other chemicals such as 4-(2-hydroxyethyl) piperazine-1-ethanesulfonic acid (HEPES), sepharose CL-4B, potassium thiocyanate (KSCN), Triton X-100, chloroform and methanol were obtained from Sigma-Aldrich Chemie GmbH (Steinheim, Germany).

Santhosh P.B., Drašler B., Drobne D., Kreft M.E., Kralj S., Makovec D, & Ulrih N.P. (2015). Effect of superparamagnetic iron oxide nanoparticles on fluidity and phase transition of phosphatidylcholine liposomal membranes. International Journal of Nanomedicine, 10, 6089-6104.

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Synthesis of γ-Fe2O3/SiO2/RhB Nanoparticles

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For the synthesis of the γ-Fe₂O₃/SiO₂/RhB NPs, reagent grade chemicals were used as received from the manufacturers. Iron (III) sulfate hydrate, iron (II) sulfate heptahydrate (ACS, 99%), citric acid (CA, 99%), tetraethoxysilane (TEOS, 99.9%) and NH₄OH (28–30%) were supplied by Alfa Aesar (Lancashire, UK). Acetone (AppliChem GmbH) and absolute ethanol (Carlo Erba, reagent - USP) were used as received. (3-Aminopropyl)triethoxysilane (APS; silane-NH₂, 99%), tetraethoxysilane (TEOS; 98%), ethyl acetate (EA), dichloromethane (DCM), dimethylformamide (DMF), Rhodamine B isothiocyanate (RhB), poly(acrylic acid) solution (25 wt. % in water) and polyvinyl pyrrolidone (PVP, 40 kDa) were obtained from Sigma-Aldrich (St. Louis, MO, USA).
Cellulose acetate (CA, Mn = 30000 g/mol) was obtained from Sigma-Aldrich and used without further purification. Acetone (Technical Grade 99.5% - Panaska Trading CO) was the solvent used in the preparation of CA polymer solutions that were further electrospun.

Petropoulou A., Kralj S., Karagiorgis X., Savva I., Loizides E., Panagi M., Krasia-Christoforou T, & Riziotis C. (2020). Multifunctional Gas and pH Fluorescent Sensors Based on Cellulose Acetate Electrospun Fibers Decorated with Rhodamine B-Functionalised Core-Shell Ferrous Nanoparticles. Scientific Reports, 10, 367.

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Recombinant FSA A129S Production and Purification

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Recombinant FSA A129S was produced and purified according to the literature with an activity of 15.0 U mg^-1 FSA (S1 Fig) [38 (link)]. N-(3-dimethylaminopropyl)-N’-ethylcarbodiimide (EDAC), α-glycerophosphate dehydrogenase–triosephosphate isomerase (GPD-TPI), D-fructose-6-phosphate (F6P) and 3-[(benzyloxycarbonyl)amino]propionaldehyde (β-CHO) were purchased from Sigma Aldrich (St. Louis, USA). Nicotinamide adenine dinucleotide (NADH) was purchased from BONTAC Bio-engineering (Shenzhen, China). 6% cross-linked agarose gels, MANA-agarose gels and High-density metal free sepharose were purchased from ABT-beads (Torrejón de Ardóz, Spain). Dihydroxyacetone (DHA) was purchased by Merck (Darmstadt, Germany). Iron (III) sulfate hydrate, iron (II) sulfate heptahydrate (ACS, 99%), citric acid (99%), tetraethoxysilane (TEOS, 99.9%), NH₄OH (28–30%) were supplied by Alfa Aesar (Lancashire, UK). (Hydroxy(polyethyleneoxy)propyl) triethoxysilane (silane-PEG), triethoxysilylpropylmaleamic acid (silane-COOH), 3-mercaptopropyltrimethoxy silane (silane-SH), N-(trimethoxysilylpropyl) ethylenediaminetriacetate (silane-EDTA) were purchased by Gelest (USA). All other reagents were commercial products of analytical grade. Silane-C≡CH and silane-N₃ were kindly supplied by Dr. Stane Pajk (Jožef Stefan Institute, Ljubljana, Slovenia) [39 (link)].

Masdeu G., Vázquez L.M., López-Santín J., Caminal G., Kralj S., Makovec D., Álvaro G, & Guillén M. (2021). Synthesis of a precursor of D-fagomine by immobilized fructose-6-phosphate aldolase. PLoS ONE, 16(4), e0250513.

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