PET/CT imaging scans were performed on a small animal PET/CT scanner (Inveon PET/CT, Siemens Medical Solutions, Knoxville, TN, USA) according to a standard protocol as described before [30 (link)]. Briefly, mice were anesthetized by isoflurane (1–3%, supplemented with oxygen) and received a mean dose of 16.03 ± 1.10 MBq [18F]FDG intravenously via a microcatheter placed in a tail vein. Static PET scans were acquired using a small animal micro PET/CT scanner (Inveon PET/CT Siemens, Knoxville, TN, USA). The PET image reconstruction method consisted of a 2-dimensional ordered subset expectation maximization algorithm (2D-OSEM) with four iterations and six subsets. Attenuation correction was performed on the basis whole body CT scan and a decay correction for [18F] was applied. PET images were corrected for random coincidences, dead time and scatter. By marking the entire tumors, starting at the edge and cutting through the whole [18F]FDG-enriched tumor, volumes and SUVs were determined. This was done by using Inveon Research Workplace 4.2 software.
Inveon pet ct
Manufactured by Siemens
Sourced in United States, Germany
Inveon PET/CT is a multimodal imaging system developed by Siemens. It combines Positron Emission Tomography (PET) and Computed Tomography (CT) technologies to provide high-resolution imaging capabilities. The system is designed for preclinical research applications.
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Lab products found in correlation
Inveon research workplace software, by Siemens (5 mentions)
Fmt scanner, by PerkinElmer (4 mentions)
Isovue 370, by Bracco (4 mentions)
Prosense 680, by PerkinElmer (4 mentions)
Vivoquant, by Invicro (2 mentions)
Inveon research workplace, by Siemens (2 mentions)
Ivis spectrum, by PerkinElmer (2 mentions)
Wizard2 automatic gamma counter, by PerkinElmer (1 mentions)
Microfil injection compound, by Flow Tech (1 mentions)
Vivotag s 750, by PerkinElmer (1 mentions)
Mmpsense 645 fast, by PerkinElmer (1 mentions)
Angiosense 680, by PerkinElmer (1 mentions)
Fmt 4000 system, by PerkinElmer (1 mentions)
Ncr nude mice, by Taconic Biosciences (1 mentions)
Osirix software, by Pixmeo (1 mentions)
Mmpsense 750 fast, by PerkinElmer (1 mentions)
Fmt 2500, by PerkinElmer (1 mentions)
Inveon research workplace 4, by Siemens (1 mentions)
Mimics software, by Materialise (1 mentions)
Matrigel, by BD (1 mentions)
68 protocols using inveon pet ct
1
Small Animal PET/CT Imaging Protocol
2
Small Animal PET/CT Imaging Protocol
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PET/CT imaging scans were performed on a small animal PET/CT scanner (Inveon PET/CT, Siemens Medical Solutions, Knoxville, TN, USA) according a standard protocol. Mice with suspected GIT (N = 5/per group) were anaesthetized using isoflurane (4% for induction and 1–2,5% maintenance during preparation and scanning) and were injected intravenously with a mean dose of 17.12 ± 1.81 MBq [18F] FDG via a custom-made micro catheter placed in the tail vein. After an uptake period of 60 min, mice were imaged in prone position for 15 min as described [17 ]. Throughout the imaging session, respiration of the mice was controlled and body temperature was constantly kept of 38 °C via a heading pad. The PET image reconstruction method consisted of a 2-dimensional ordered subset expectation maximization algorithm (2D-OSEM) with four iterations and 6 subsets. Attenuation correction was performed on the basis whole body CT scan and a decay correction for [18F] was applied. PET images were also corrected for random coincidences, dead time and scatter. Tumor volumes and SUVs were determined using Inveon Research Workplace 4.2 software.
3
In Vivo Fatty Acid Metabolism PET Imaging
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Ten days after intracardiac injection of Bo1 cells, FF mice were subjected to PET imaging using cross-calibrated Siemens Inveon PET/CT or Focus F220 scanners. Mice were secured in a custom-designed acrylic restraining device and placed inside the field of view of the scanners. A 30-minute dynamic PET image acquisition was initiated immediately preceding a bolus injection of 11C-palmitate via the tail vein. Dynamic images were reconstructed using Ordered Subsets Expectation Maximization algorithm. Images were analyzed by drawing ROIs on livers to ascertain in vivo measures of fatty acid (via 11C-palmitate) metabolism. Data derived from ROIs were normalized to standardized uptake values (SUV = activity × [weight of mouse/injected dose]) to account for differences in injected dose and weight of mice.
4
PET Imaging of Lung Cancer Xenografts
5
Micro-CT Analysis of Vascular Networks
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The micro-CT system (Siemens Inveon PET/CT, Berlin, German) was used to assess vascularity. Vascular networks around the shoulder junction were examined using micro-CT analyses combined with perfusion of a contrast agent. Briefly, the blood vessels were first rinsed with normal saline containing heparin and 4% paraformaldehyde (PFA). Then, using MICROFIL® injection compound (Flow Tech, Inc., Carver, MA) contrast media, a radio-opaque silicone rubber compound containing lead chromate was perfused via the heart. After perfusion, the reconstruction shoulder was removed and scanned using the micro-CT system. The samples were subsequently decalcified for 30 days using a 10% EDTA solution. After complete decalcification, the samples were scanned again to visualize only the vascularization within the callus tissue. Three-dimensional reconstructions were made using the Inveon research workplace (2.0, Berlin, German).
6
PET-CT Imaging of Tumor Uptake
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As previously described, small animal PET-CT imaging was performed 14 days after tumour inoculation using a Siemens Inveon PET-CT [18 (link)]. Mice were anaesthetised using isoflurane gas (maintained at 1.2% alveolar concentration) and injected with [18F]AlF-mNOTA-GZP (~15 MBq) via the lateral tail vein. A 10-min static PET acquisition was performed at 60 min post-injection (p.i.) of the tracer, and a CT scan was acquired for anatomical co-registration. Animals were monitored during imaging using the BioVet physiosuite for their body temperature and respiration rate. The reconstruction and the data analysis were performed as described previously [17 (link),18 (link)] using Amide software (version 10.3 Sourceforge). The radiotracer uptake in tissues was determined and normalised to injected dose and converted to a percentage of injected dose per gram of tissue (% ID/g).
7
Preclinical 18F-FDG PET/CT Imaging
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Mice fasted overnight prior to 18FDG-PET/CT on an Inveon PET/CT (Siemens Molecular Imaging, Knoxville, TN, USA). On the day of scanning, mice were briefly anesthetized with isoflurane prior to injection of 3.7 MBq 18F-FDG via the lateral tail vein. They were allowed to roam in a cage during a one-hour uptake period. They were re-anesthetized and maintained under isoflurane anesthesia throughout the PET/CT. Whole body CT images were obtained at 80 kV with a focal spot of 58 μm and a binning factor of 1:4. A whole body PET was then obtained.
8
Small Animal PET/CT Tumor Measurement
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The tumor size was measured via PET/CT measurements on a small animal PET/CT scanner (Inveon PET/CT, Siemens Medical Solutions, Knoxville, TN, USA) in accordance to a standard protocol as described before.25 (link) Mice were anesthetized using isoflurane and received FDG intravenously. Evaluation of the images was performed as described before.8 (link) Treatment outcome determined according to clinical staging as follows: (I) progressive disease (PD) = tumor volume >25% vs. baseline (= day 0); (II) stable disease (SD): tumor volume similar to initial staging (≤25% vs. day 0); (III) partial response (PR): tumor volume 50% lower or more vs. baseline (=day 0). Follow-up was done at day 30 (= short-term) and, in some cases, at ~day 50 (= long-term follow-up).
9
Multimodal Imaging of Vascular Targeting
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Blood clearance, organ distribution and preliminary imaging studies used IVIS Spectrum (Caliper LifeSciences) together with micro-CT (Inveon PET-CT, Siemens). Subsequently we used an FMT 4000 system (PerkinElmer, Massachusetts USA) equipped with four lasers. Animals were injected with a VivoTag-S 750-MAL-conjugated antibody, as well as in selected experiments with MMPSense-645-FAST (PerkinElmer, MA) to evaluate MMP activity or AngioSense 680 (PerkinElmer, MA) to identify intravascular space. Contrast-enhanced high resolution CT localized the aortic root and major arteries in the neck and thorax to guide the placement of the region of interest (ROI) for the fluorescence activity map concomitantly obtained by FMT. Fluorescence and CT image fusion relied on fiducial markers present on the imaging cartridge, and used Amide 1.0.4 (Sourceforge.net).
10
Prophylactic and Therapeutic Tumor Vaccination Protocol
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A detailed treatment schedule is provided in Figure 1. Brie y, prophylactic application was initiated when mice aged 8-10 weeks by four weekly boosts of tumor lysates (10mg/kg bw, s.c., 328 vaccine: n=10; A7450 T1 M1 vaccine: n=9, respectively) followed by monthly applications (a total of 12 vaccinations). Control mice were left untreated (n=15 mice). For the therapeutic vaccination approach, mice were given four weekly boosts. Vaccination was sustained (10mg/kg bw, biweekly) until tumors progressed (max.12 injections; n = 8 mice/group). Control mice were left untreated (n = 7 mice). Reduction of suffering during the trial was guaranteed by providing daily prepared soaked pellets, twice-daily monitoring of the health status using a score sheet and by applying humane endpoints (weight loss, any sign of pain or distress, or changes in social behavior). All mice were sacri ced before they became moribund to prevent pain and distress.
Positron Emission Tomography/Computed Tomography (PET/CT) imaging PET/CT imaging scans were performed on a small animal PET/CT scanner (Inveon PET/CT, Siemens Medical Solutions, Knoxville, TN, USA) according to a standard protocol as described before [19, 20] .
Positron Emission Tomography/Computed Tomography (PET/CT) imaging PET/CT imaging scans were performed on a small animal PET/CT scanner (Inveon PET/CT, Siemens Medical Solutions, Knoxville, TN, USA) according to a standard protocol as described before [19, 20] .
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