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Oasis max cartridge column

Manufactured by Waters Corporation

The Oasis MAX cartridge column is a laboratory equipment product by Waters Corporation. It is designed for solid-phase extraction (SPE) applications. The core function of the Oasis MAX cartridge column is to facilitate the separation, purification, and concentration of analytes from complex sample matrices.

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2 protocols using oasis max cartridge column

1

Quantification of Polysorbate 20 by HPLC

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High-performance liquid chromatography was performed on an Agilent Infinity 1260 setup equipped with a temperature-controlled autosampler, column oven, binary gradient pump and evaporative light scattering detector (ELSD, 380-LS, Varian). The ELSD was used with a gas flow rate of 1 standard liter per minute (SLM), nebulizer temperature of 45°C and evaporation temperature of 100°C. The column temperature was set to 30°C. A gradient using two mobile phases as described earlier was used (15 (link)). In brief, mobile phase A contained 2% formic acid in water and mobile phase B 2% formic acid in isopropanol. Intact polysorbate 20 was quantified by loading 20 μL of sample onto a Waters Oasis MAX cartridge column (20 mm × 2.1 mm, particle size 30 μm). The flow rate was set to 1 mL/min starting with 90% of mobile phase A. After 1 min, mobile phase B was increased to 20%. After 3.4 min, mobile phase B was increased to 80% and after 3.5 min, to 100%, where it was kept for 0.9 min. After 4.7 min, mobile phase B was set to the initial condition of 10%. Intact polysorbate 20 elutes as a single peak at approximately 4.6 min.
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2

Quantifying Kauranoic Acid in Plants

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Three sets of frozen plant tissues (300 mg) were homogenized five times with 1500 rpm for 30 s in 80% (v/v) MeOH containing 5% (v/v) FA as the extraction solution containing one 10 mm stainless bead using Shake Master NEO (Bio Medical Science, Tokyo, Japan). d2-KA (10 ng) was added to the extraction solution as an internal standard. The homogenized samples were spun (200 rpm) at 4 °C overnight. The homogenates were then centrifuged ( 1570×g, 5 min, 4 °C) and endogenous KA was re-extracted from the resulting pellets in the same solution twice. The combined extracts were evaporated to a water phase using a vacuum evaporator centrifuge (Iwaki Glass, Chiba, Japan) and purified using an Oasis MCX cartridge column (60 mg, 3 cc, Waters) activated with MeOH and pre-equilibrated with 5 mM FA. The evaporated samples were dissolved in 5 mM FA and loaded onto the cartridges, which were washed with 5 mM FA. Columns were then run to dryness, and KA was eluted with MeOH. Elutes were evaporated to dryness. The sample was dissolved in 25 mM NH4HCO3 before loading onto an Oasis MAX cartridge column (60 mg, 3 cc, Waters). The column was first activated with MeOH then equilibrated with 25 mM NH4HCO3 before loading the sample. Columns were then washed with MeOH and KA was eluted with 0.2 M FA in MeOH, which was evaporated to dryness.
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