The Calcein-AM/PI staining kit (BestBio) was used to assay the viability of cyt c+/+ and cyt c–/– cells. Cells were harvested at the confluence of 70–80%, transfered to 96-well plate and stained with Calcin-AM and PI. Live cells and dead cells showed green fluorescence and red fluorescence respectively.
Calcein am pi staining kit
Manufactured by BestBio
Sourced in China
The Calcein-AM/PI staining kit is a fluorescent dye-based assay designed to assess cell viability. The kit contains Calcein-AM, a non-fluorescent, cell-permeable compound that becomes fluorescent upon hydrolysis by intracellular esterases, and Propidium Iodide (PI), a dye that can only enter cells with compromised membranes. Together, these two dyes allow for the differentiation between live (Calcein-AM positive) and dead (PI positive) cells.
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3 protocols using calcein am pi staining kit
1
Cell Viability Assay with Calcein-AM/PI
2
Cell Culture and Staining Protocols
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All the ultra-pure water used in the experiments was purified by a Mili-Q A10 filtration system (Milipore, Billerica, MA, United States). Phosphate-buffered saline (PBS) was obtained from Solarbio (Beijing, China). Low glucose Dulbecco’s Modified Eagle’s Medium (LGDMEM), fetal bovine serum (FBS), and streptomycin double antibody were obtained from Gibco (Grand Island, NY, United States). Hematoxylin and Eosin (H&E) stain dye solution was purchased from Thermo Fisher Scientific Co., Ltd. (Shanghai, China). Calcein-AM-PI staining kit were obtained from Bestbio (Nanjing China). Rhodamine-phalloidin was obtained from Solarbio (Beijing, China). Opioid receptor antibody, the voltage-gated Na (+) channel subtype Nav1.7 antibody, and the P substance receptor antibody were purchased from Abcam (MA, United States). Goat antirabbit IgG were purchased from the Life Technologies (United States).
3
Assessing Isolated Cancer Cell Viability
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The Calcein-AM/PI staining kit (BestBio)
was used to assay the viability of isolated cancer cells. Live cells
and dead cells showed green and red fluorescence, respectively. For
the verification of cell proliferation ability before and after filtration,
the isolated cancer cells were cultured at 37 °C in a humidified
incubator (5% CO2). The number of cells was counted at
days 1, 2, 3, 4, and 5, and the growth curve was plotted and fit by
a growth formula.
was used to assay the viability of isolated cancer cells. Live cells
and dead cells showed green and red fluorescence, respectively. For
the verification of cell proliferation ability before and after filtration,
the isolated cancer cells were cultured at 37 °C in a humidified
incubator (5% CO2). The number of cells was counted at
days 1, 2, 3, 4, and 5, and the growth curve was plotted and fit by
a growth formula.
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