Alexa fluor rhodamine phalloidin

The following antibodies and stains were used: Alexa Fluor Rhodamine Phalloidin (Invitrogen Cat.#: R415; 1:50), Hoechst (Invitrogen Cat.#: H3570; 1:10,000), Rat anti-HA (Roche Cat.#: 1186742300; 1:200–1:100), rabbit anti-mouse caspase-3 (R+D systems Cat.#: AF835; 1:100), rat anti-mouse Cd104 (β4-integrin) (BD Biosciences Cat.#: 553745; 1:100), Rat anti-mouse α-18 (gift from Akira Nagafuchi, [25 (link)]; 1:100), Rabbit anti- mouse Olfm4 (Cell Signaling Technology, Cat.#:39141T; 1:400), Rabbit anti- mouse SOX9 (Millipore, Cat.#: AB5535; 1:250), mouse anti- Phospho-Histone H2A.X (Invitrogen, Cat.#: MA1-2022; 1:100), Rabbit anti-mouse non muscle myosin heavy chain IIA (Biolegend, Cat.#: 909001; 1:100), Rabbit anti-mouse non muscle myosin heavy chain IIC (Biolegend, Cat.#: 919201; 1:100), Rat anti-mouse Ki67 (Invitrogen, Cat.# 14-5698-80; 1:100), Rabbit anti-mouse GRASP65 (Abcam, Cat.# ab30315; 1:200), Rat anti-mouse E-cadherin (Invitrogen, Cat.# 13–1900; 1:500), Rabbit anti-Pericentrin (Abcam, Cat.# ab4448; 1:100), Rabbit anti-human ZO-1 (Invitrogen, Cat.# 61–7300; 1:100).

The following antibodies and stains were used: Alexa Fluor Rhodamine Phalloidin (Invitrogen^™ Cat.#: R415; 1:50), Hoechst (Invitrogen^™ Cat.#: H3570; 1:10,000), Rat anti-HA (Roche Cat.#: 1186742300; 1:100), rabbit anti-mouse caspase-3 (R+D systems Cat.#: AF835; 1:100), rat anti-mouse Cd104 (b4integrin) (BD Biosciences Cat.#: 553745; 1:100), Rat anti-mouse α-18 (gift from Akira Nagafuchi, [25 (link)]; 1:100), Rabbit anti- mouse SOX9 (Millipore, Cat.#: AB5535; 1:250), mouse anti- Phospho-Histone H2A.X (Invitrogen, Cat.#: MA1-2022; 1:100), Rabbit anti-mouse non muscle myosin heavy chain IIC (Biolegend, Cat.#: 919201; 1:100), Rat anti-mouse Ki67 (Invitrogen, Cat.# 14-5698-80; 1:100), Rabbit anti-mouse GRASP65 (Abcam, Cat.# ab30315; 1:200).