Qiasymphony rna kit

Manufactured by Qiagen

Sourced in Germany, United States

The QIAsymphony RNA Kit is a lab equipment product designed for the automated purification of high-quality RNA from a variety of sample types. The kit utilizes QIAGEN's proven technology to provide reliable and efficient RNA extraction.

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Lab products found in correlation

Agilent bioanalyzer, by Agilent Technologies (10 mentions) Qiasymphony dna mini kit, by Qiagen (9 mentions) Infinium qc array, by Illumina (7 mentions) Quantinova probe rt pcr kit, by Qiagen (6 mentions) Qiasymphony sp, by Qiagen (5 mentions) Rneasy mini kit, by Qiagen (5 mentions) Nanodrop 8000, by Agilent Technologies (4 mentions) Rlt plus buffer, by Qiagen (3 mentions) Quantinova sybr green rt pcr kit, by Qiagen (3 mentions) Lightcycler 480 real time pcr system, by Roche (3 mentions) Agilent rna 6000 nano kit, by Agilent Technologies (3 mentions) Novaseq 6000, by Illumina (3 mentions) Paxgene tube, by Qiagen (2 mentions) Nebnext globin and rrna depletion kit, by New England Biolabs (2 mentions) Nebnext ultra directional library prep kit, by New England Biolabs (2 mentions) Tissuelyser 2, by Qiagen (2 mentions) Qiasymphony sp instrument, by Qiagen (2 mentions) Bcl2fastq tool, by Illumina (2 mentions) Primescript rt reagent kit, by Takara Bio (2 mentions) Qiaamp viral rna mini kit, by Qiagen (2 mentions)

Spelling variants of this product

QIAsymphony (189 citations) QIAsymphony PAXgene Blood RNA Kit (13 citations) QIAsymphony RNA (5 citations) QIAsymphony kits (2 citations)

59 protocols using qiasymphony rna kit

Comprehensive Genomic Profiling of Tumor Samples

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Our study sampled a single site of the primary tumor from surgical resections, due to the internal requirement to process a minimum of 125mg of tumor issue and 50mg of adjacent normal tissue. DNA and RNA were extracted from tumor and adjacent normal specimens in a co-isolation protocol using QIAGEN’s QIAsymphony DNA Mini Kit and QIAsymphony RNA Kit. Genomic DNA was also isolated from peripheral blood (3−5mL) to serve as matched normal reference material. The Qubit dsDNA BR Assay Kit was used with the Qubit® 2.0 Fluorometerto determine the concentration of dsDNA in an aqueous solution. Any sample that passed quality control and produced enough DNA yield to go through various genomic assays was sent for genomic characterization. RNA quality was quantified using both the NanoDrop 8000 and quality assessed using Agilent Bioanalyzer. A sample that passed RNA quality control and had a minimum RIN (RNA integrity number) score of 7 was subjected to RNA sequencing. Identity match for germ-line, normal adjacent tissue, and tumor tissue was assayed at the BCR using the Illumina Infinium QC array. This beadchip contains 15,949 markers designed to prioritize sample tracking, quality control, and stratification. The genomic DNA and total RNA extraction were only applied to a subset of adjacent normal tissues without enrichment for endometrium.

Dou Y., Kawaler E.A., Zhou D.C., Gritsenko M.A., Huang C., Blumenberg L., Karpova A., Petyuk V.A., Savage S.R., Satpathy S., LiU W., WU Y., Tsai C.F., Wen B., Li Z., Cao S., Moon J., Shi Z., Cornwell M., Wyczalkowski M.A., Chu R.K., Vasaikar S., Zhou H., Gao Q., Moore R.J., Li K., Sethuraman S., Monroe M.E., Zhao R., Heiman D., Krug K., Clauser K., Kothadia R., Maruvka Y., Pico A.R., Oliphant A.E., Hoskins E.L., Pugh S.L., Beecroft S.J., Adams D.W., Jarman J.C., Kong A., Chang H.Y., Reva B., Liao Y., Rykunov D., Colaprico A., Chen X.S., Czekański A., Jędryka M., Matkowski R., Wiznerowicz M., Hiltke T., Boja E., Kinsinger C.R., Mesri M., Robles A.I., Rodriguez H., Mutch D., Fuh K., Ellis M.J., DeLair D., Thiagarajan M., Mani D.R., Getz G., Noble M., Nesvizhskii A.I., Wang P., Anderson M.L., Levine D.A., Smith R.D., Payne S.H., Ruggles K.V., Rodland K.D., Ding L., Zhang B., Liu T, & Fenyö D. (2020). Proteogenomic Characterization of Endometrial Carcinoma. Cell, 180(4), 729-748.e26.

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RNA Extraction and RNA-Seq of ACSA-2+ Astrocytes

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RNA from ACSA-2⁺ astrocytes was extracted on the QIAsymphony SP (Qiagen Corporation, Germany) using the QIAsymphony RNA Kit (Qiagen, 931636) and the protocol DNF-472T33 - HS Total RNA 15nt.mthds. The resulting RNA was eluted with RNase free water and stored at −80°C. Total RNA (500 ng) was used as input for library preparation. Three biological replicates for ACSA-2⁺ astrocytes isolated from CL and IL ischemic hemispheres were sequenced on an Illumina HiSeq 2500 using High Output V4 chemistry and single read 100 bases format.

Song S., Huang H., Guan X., Fiesler V., H. Bhuiyan M.I., Liu R., Jalali S., Hasan M.N., Tai A.K., Chattopadhyay A., Chaparala S., Sun M., Stolz D.B., He P., Agalliu D., Sun D, & Begum G. (2020). Activation of endothelial Wnt/β-catenin signaling by protective astrocytes repairs BBB damage in ischemic stroke. Progress in neurobiology, 199, 101963.

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RNA-seq of Whole Blood Transcriptome

Cited 1 time

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Blood draws were performed in the morning under fasting conditions. Approximately 2.5 mL of whole blood were kept frozen at −80^oC in a PAXgene tube (QIAGEN, 761115) until processing (Jefferson et al., 2016 (link)). RNA extraction, library preparation, and RNA sequencing were performed by the VANTAGE Core (Vanderbilt University, TN, USA). Total RNA was extracted from whole blood using the QIASymphony RNA Kit (QIAGEN, 931636), and both ribosomal RNA and hemoglobin were depleted with the NEBNext Globin and rRNA Depletion Kit (New England BioLabs, Inc., E7750). Library preparation was completed using the NEBNext Ultra Directional Library Prep Kit (New England BioLabs, Inc., E7420) before sequencing was performed using 150 base pair (bp) paired end reads on an Illumina NovaSeq 6000 (Illumina), targeting an average of 50 million reads per sample.

Seto M., Weiner R.L., Dumitrescu L., Mahoney E.R., Hansen S.L., Janve V., Khan O.A., Liu D., Wang Y., Menon V., De Jager P.L., Schneider J.A., Bennett D.A., Gifford K.A., Jefferson A.L, & Hohman T.J. (2022). RNASE6 is a novel modifier of APOE-ε4 effects on cognition. Neurobiology of aging, 118, 66-76.

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SARS-CoV-2 Viral Load Quantification

Cited 2 times

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RNA was extracted from infected Caco2 cells using the QIAsymphony RNA kit (Qiagen, Germany), and RNA was extracted from hamster lung tissues using the RNeasy Mini kit (Qiagen, Germany). Viral gene copies of SARS-CoV-2 was quantified by the RNA-dependent RNA polymerase (RdRp) using the QuantiNova Probe RT-PCR kit (Qiagen, Germany) as previously described 28 (link), 29 (link).

Yuen T.T., Chan J.F., Yan B., Shum C.C., Liu Y., Shuai H., Hou Y., Huang X., Hu B., Chai Y., Yoon C., Zhu T., Liu H., Shi J., Zhang J., Cai J.P., Zhang A.J., Zhou J., Yin F., Yuan S., Zhang B.Z, & Chu H. (2022). Targeting ACLY efficiently inhibits SARS-CoV-2 replication. International Journal of Biological Sciences, 18(12), 4714-4730.

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Total RNA Purification Protocol

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Approximately 30–50 mg of each tissue was homogenized in 800 μl of Qiagen Buffer RLT Plus lysis reagent via bead-milling on the TissueLyser II (Qiagen, Hilden, Germany). Total RNA was purified following the large volume protocol for the QIASymphony RNA Kit on the QIAsymphony SP as described by the manufacturer (Qiagen). Purified total RNA was quantified by UV spectrophotometry using the DropSense 96 Microplate Spectrophotometer (PerkinElmer, Waltham, MA, USA) and the purity was assessed based on the A260/A280 and A260/A230 ratios. An aliquot of the RNA was assessed for quality by RNA ScreenTape on an Agilent 2200 TapeStation (Agilent Technologies, Santa Clara, CA, USA).

Motomura K., Romero R., Tarca A.L., Galaz J., Bhatti G., Done B., Arenas-Hernandez M., Levenson D., Slutsky R., Hsu C.D, & Gomez-Lopez N. (2020). Pregnancy-specific transcriptional changes upon endotoxin exposure in mice. Journal of perinatal medicine, 48(7), 700-722.

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RNA Extraction from Muscle Tissue

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Muscle samples were homogenized in 800 µl of buffer RLT plus (Qiagen, USA) plus 2 steel balls using a Tissue Lyser (Qiagen, USA). Cell debris were removed by centrifugation and clear lysates were placed in the QIAsymphony SP workstation (Qiagen, USA). RNA was extracted with the QIAsymphony RNA kit (Qiagen cat# 931636) following manufacturer’s procedures.
RNA was quantified and checked for purity on a Nanodrop-8000. RNA integrity was controlled using RNA 6000 Nano LabChip kit (Agilent technologies cat# 5065-4476) on an Agilent Bioanalyzer (Agilent technologies).

Andreux P.A., van Diemen M.P., Heezen M.R., Auwerx J., Rinsch C., Groeneveld G.J, & Singh A. (2018). Mitochondrial function is impaired in the skeletal muscle of pre-frail elderly. Scientific Reports, 8, 8548.

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Absolute Quantification of miR-122 Expression

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Extraction of total RNA and microRNA was performed using the QIAsymphony RNA Kit (Qiagen) according to the manufacturer's protocol. In addition, the samples were treated with DNAse to avoid contamination by genomic or mitochondrial DNA. A total of 10 ng of RNA was used for cDNA preparation using the MiScript II RT Kit (Qiagen) and the mmu-miR-122 transcript levels were measured by qPCR on the LightCycler480 (Roche), using the miScript SYBR Green PCR kit with mmu-miR-122 miScript primer assay (Qiagen). According to the supplier's protocol, MicroRNA expression was determined as absolute quantification. To estimate the absolute copy number of miR-122, a standard range was established by 10-fold dilution over 10 points using mus musculus miR-122 (mmu-miR-122) mimic (Dharmacon). The dilution range used for reverse-transcription with miScript Reverse Transcription kit (Qiagen) started from 7.13 10¹¹ copies per ng of total RNA for mmu-miR-122 mimic. Copy number of miR-122 per ng of total RNA in each sample was determined by plotting Ct values in the mmu-miR-122 standard curves.

Gasmi I., Machou C., Rodrigues A., Brouillet A., Nguyen T.C., Rousseau B., Guillot A., Rodriguez C., Demontant V., Ait-Ahmed Y., Calderaro J., Luciani A., Pawlotsky J.M, & Lafdil F. (2022). Interleukin-17 programs liver progenitor cell transformation into cancer stem cells through miR-122 downregulation with increased risk of primary liver cancer initiation. International Journal of Biological Sciences, 18(5), 1944-1960.

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Viral RNA Extraction and Quantification

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The infected cells were lysed with 90 μL RLT buffer then viral RNA was extracted and eluted in 100 μL DNase/RNase-free water using QIAsymphony RNA Kit (931636, Qiagen, Germantown Road Germantown, MD, USA). Viral RNA from mice lung and nasal turbinate samples were extracted with the RNeasy Mini kit (74106, Qiagen). Viral subgenomic RNA was detected using primers targeting the subgenomic E gene with the QuantiNova Probe RT-PCR Kit (208354, Qiagen). The primer and probe sequences are available upon request.

Chan J.F., Hu B., Chai Y., Shuai H., Liu H., Shi J., Liu Y., Yoon C., Zhang J., Hu J.C., Hou Y., Huang X., Yuen T.T., Zhu T., Li W., Cai J.P., Luo C., Yip C.C., Zhang A.J., Zhou J., Yuan S., Zhang B.Z., Huang J.D., To K.K., Yuen K.Y, & Chu H. (2022). Virological features and pathogenicity of SARS-CoV-2 Omicron BA.2. Cell Reports Medicine, 3(9), 100743.

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DRAXIN Expression Analysis in Glioma

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RT-qPCR was used to detect the expression of DRAXIN in glioma tissues and glioma cell lines. Tissue RNA and cellular RNA were extracted with Tri-Reagent and QIA symphony™ RNA kit (QIAGEN, Germany). Subsequently, the RNA solubility was determined using an ultra-micro spectrophotometer (Thermo Fisher, USA). The NovoStart SYBR qPCR SuperMix Plus (Novoprotein, China) kit was used to perform RT-qPCR. GADPH was used as an internal reference. The primer sequences used for DRAXIN were 5′-CGACTGGACCGATTATGAAGAC-3′ (F) and 5′-CGGCTGGTGATGTTTCGTTAC-3′ (R). “2^−ΔΔCT” or “2^−ΔCT” methods were individually applied to analyze the DRAXIN expression of glioma cells and tissues. The unpaired t test was used for the statistical difference between the two groups. When the p value was < 0.05, the difference between the two groups was statistically significant.

Jia Y., Liu Z., Cheng X., Liu R., Li P., Kong D., Liang W., Liu B., Wang H., Bu X, & Gao Y. (2022). DRAXIN as a Novel Diagnostic Marker to Predict the Poor Prognosis of Glioma Patients. Journal of Molecular Neuroscience, 72(10), 2136-2149.

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Comprehensive tumor profiling protocol

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Our study sampled a single site of the primary tumor from surgical
resections, with an internal requirement to process a minimum of 125mg of
tumor issue and 50mg of NAT. DNA and RNA were extracted from tumor and NAT
specimens in a co-isolation protocol using Qiagen’s QIAsymphony DNA
Mini Kit and QIAsymphony RNA Kit. Genomic DNA was also isolated from
peripheral blood (3–5mL) to serve as matched normal reference
material. The Qubit™ dsDNA BR Assay Kit was used with the
Qubit® 2.0 Fluorimeter to determine the concentration of dsDNA in an
aqueous solution. Any sample that passed quality control and produced enough
DNA yield to go through the multiple planned genomic assays was sent for
genomic characterization. RNA quality was quantified using the NanoDrop 8000
and quality assessed using an Agilent Bioanalyzer. A sample of sufficient
quantity that passed RNA quality control and had a minimum RIN (RNA
integrity number) score of 7 was subjected to RNA sequencing. Identity
matches for germline, normal adjacent tissue, and tumor tissue were
confirmed at the BCR using the Illumina Infinium QC array. This beadchip
contains 15,949 markers designed to prioritize sample tracking, quality
control, and stratification.

Gillette M.A., Satpathy S., Cao S., Dhanasekaran S.M., Vasaikar S.V., Krug K., Petralia F., Li Y., Liang W.W., Reva B., Krek A., Ji J., Song X., Liu W., Hong R., Yao L., Blumenberg L., Savage S.R., Wendl M.C., Wen B., Li K., Tang L.C., MacMullan M.A., Avanessian S.C., Kane M.H., Newton C.J., Cornwell M., Kothadia R.B., Ma W., Yoo S., Mannan R., Vats P., Kumar-Sinha C., Kawaler E.A., Omelchenko T., Colaprico A., Geffen Y., Maruvka Y.E., da Veiga Leprevost F., Wiznerowicz M., Gümüs Z.H., Veluswamy R.R., Hostetter G., Heiman D.I., Wyczalkowski M.A., Hiltke T., Mesri M., Kinsinger C.R., Boja E.S., Omenn G.S., Chinnaiyan A.M., Rodriguez H., Li Q.K., Jewell S.D., Thiagarajan M., Getz G., Zhang B., Fenyö D., Ruggles K.V., Cieslik M.P., Robles A.I., Clauser K.R., Govindan R., Wang P., Nesvizhskii A.I., Ding L., Mani D.R, & Carr S.A. (2020). Proteogenomic characterization reveals therapeutic vulnerabilities in lung adenocarcinoma. Cell, 182(1), 200-225.e35.

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