Alpha spectrophotometer

The phenoxazine ether derivatives were prepared based on the adoption of method by Attila et al. (1999 (link)). The reagents used in this work were of analytical grades, and were bought from Sigma Aldrich chemical company, Germany and Fluka Chemical Company. They were used as supplied with no additional purification. Fisher-Johns melting points apparatus was used for the determination of the melting points of the synthesized compounds, and the figures were uncorrected. UV/visible, IR, and ¹H NMR spectroscopy were used to characterize the compounds. UV-visible spectra were determined in water on a JENWAY 6645 UV/VIS spectrophotometer. The absorption maxima are reported in nanometers, and the log ε-values are indicated in the parenthesis. Infrared spectra in wave numbers (cm⁻¹) were determined on an alpha Bruker spectrophotometer. Nuclear magnetic resonance (¹H NMR) spectra were determined using a Jeol 7000MH spectrometer at University of New Castle, London, United Kingdom. Chemical shifts were recorded on the delta (δ) scale. Interpretation of spectral data was done with reference to literature (Mohan, 2000 ).

Chemicals were purchased from Sigma-Aldrich Company (Chemie GmbH, 82024 Taufkirchen, Germany). The CHN analyses were determined using a Perkin-Elmer 2400 instrument (PerkinElmer, Inc., 940 Winter Street, Waltham, MA, USA). Cobalt content was determined using Shimadzu atomic absorption spectrophotometer (AA-7000 series, Shimadzu, Ltd., Kyoto, Japan). An Alpha Bruker spectrophotometer (Billerica, MA, USA) was used to measure the FTIR spectra in KBr pellets (Figures S4 and S5, Supplementary Data). The FTIR spectra were recorded in the range of 4000–400 cm⁻¹ at a spectral resolution of 2 cm⁻¹ and with 40 scans. The UV-Vis electronic spectra were recorded in ethanol using Pg instruments T80+ spectrophotometer (Alma Park, Wibtoft, UK). The melting points were ascertained in open capillary tubes using a Gallenkamp melting point apparatus (Sigma-Aldrich Chemie GmbH, Taufkirchen, Germany) and were uncorrected.

All chemicals and solvents were purchased from Sigma-Aldrich and Merck (Saint Louis, MO, USA), were of reagent grade, and were used without further purification, except TiCl₄, which was distilled under high vacuum just prior to use. C, H, and N analyses were conducted by the microanalytical service of the School of Chemistry, the University of Glasgow. FT-IR transmission spectra of the compounds, in KBr pellets, were acquired using a Bruker Alpha spectrophotometer (Bruker, Billerica, MA, USA) in the 4000−400 cm⁻¹ range. The UV−Vis diffuse reflectance spectra were recorded at room temperature on an Agilent Cary 60 UV−Vis spectrophotometer (Agilent Technologies, Santa Clara, CA, USA). The UV–Vis and the luminescence solution spectra were acquired on a Shimadzu UV-2600i UV–Vis Spectrophotometer (Shimadzu, Nagoya, Japan) and on a Jasco Spectrofluorometer FP-8300 (JASCO, Mary’s Court Easton, MD 21601, USA), respectively, at room temperature.