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4 protocols using chlorfenapyr

1

Pesticide Effects on Mitochondrial Function

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Mitochondrial pesticides chlorfenapyr (CAS Number 122453-73-0), hydramethylnon (CAS Number 67485-29-4), pyridaben (CAS Number 96489-71-3), tolfenpyrad (CAS Number 129558-76-5), and fenazaquin (CAS Number 120928-09-8) and non-mitochondrial pesticides imidacloprid (CAS Number 138261-41-3), deltamethrin (CAS Number 52918-63-5), malathion (CAS Number 121-75-5), metribuzin (CAS Number 21087-64-9), penconazole (CAS Number 66246-88-6), cypermethrin (CAS Number 52315-07-8), and esfenvalerate (CAS Number 66230-04-4) were from Sigma-Aldrich, USA. All pesticides were dissolved in dimethyl sulfoxide (DMSO) at a concentration of 10 mM.
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2

Insecticide Evaluation and Characterization

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The insecticides and chemicals utilized for this study were analytical-grade standards. Broflanilide (Chemical Abstracts Service [CAS] #1207727-04-5), tetraniliprole (CAS #1229654-66-3), chlorantraniliprole (CAS #500008-45-7), chromafenozide (CAS #143807-66-3), and spinetoram (CAS #187166-40-1) were purchased from Dr. Ehrenstorfer (Augsburg, Germany). Cyantraniliprole (CAS #736994-63-1), flubendiamide (CAS #272451-65-7), pyridalyl (CAS #179101-81-6), metaflumizone (CAS #139968-49-3), chlorfenapyr (CAS #122453-73-0), abamectin (CAS #71751-41-2), dimethyl sulfoxide (DMSO) (CAS #67-68-5), and Triton X-100 (CAS #9002-93-1) were purchased from Sigma Aldrich (Shanghai, China).
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3

Acaricide Sources and Purity

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All acaricides were technical grade and were obtained from the following sources: fenothiocarb (99.8%, Sigma Aldrich, Saint Louis, Mo) monocrotophos (99.5%, Chem Service, West Chester, PA), omethoate (97.0%, Sigma Aldrich), endosulfan (99.4%, Sigma Aldrich), bifenthrin (99.0%, Sigma Aldrich), fenpropathrin (99.5%, Chem Service, West Chester, PA), Abamectin (97.6%, Sigma Aldrich), etoxazole (98.5%, Sigma Aldrich), fenbutatin oxide (97.7%, Sigma Aldrich), chlorfenapyr (99.6%, Sigma Aldrich), flufenoxuron (98.4%, Sigma Aldrich), amitraz (96.8%, Sigma Aldrich), pyridaben (99.7%, Sigma Aldrich), tebufenpyrad (99.8%, Sigma Aldrich), spiromesifen (99.8%, Sigma Aldrich), (EZ)-cyenopyrafen (97.3%, Sigma Aldrich), cyflumetofen (96.0%, Wako Pure Chemical Industries, Ltd., Japan), bifenazate (99.9%, Sigma Aldrich) and dicofol (97.2%, Sigma Aldrich).
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4

Validated Analytical Method for Chlorfenapyr and Methomyl

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The certified reference standard of chlorfenapyr and methomyl (purity>98%) were purchased from Sigma-Aldrich Co. chlorfenapyr (Challenger Super™ 24% SC), was supplied by BASF Co. Ltd. methomyl (Neomyl™ 90% SP), was supplied by kz pesticides and chemical Co. The other chemicals and reagents of the analytical grade used such as acetonitrile, HPLC grade (POUCH SA, Gliwice, Poland); acetic acid (El Nasr Pharmaceutical Chemicals Co., Abu-Zaabal, Cairo, Egypt); primary secondary amine (PSA), graphitized carbon black (GCB), C18, anhydrous magnesium sulfate (MgSO4) and anhydrous sodium acetate involved in QuEChRs kits purchased from Agilent Technologies Co., USA).
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