The effects of PLA2G7 silencing on cancer cell viability and induction of apoptosis were assessed with the CellTitre‐Glo cell viability assay and ApoONE apoptosis assay (Promega Inc, Madison, WI, USA) according to the manufacturer's instructions. The EnVision multilabel plate reader (Perkin‐Elmer, Waltham, MA) was used for signal quantification. Scrambled siRNA or shRNA was used as the negative control and KIF11 siRNA as the positive control.
Celltitre glo cell viability assay
Manufactured by Promega
Sourced in United States
The CellTiter-Glo cell viability assay is a reagent-based luminescent assay that quantifies the amount of ATP present in metabolically active cells. The assay measures the light signal generated from the luciferase reaction, which is proportional to the amount of ATP and can be used to determine the number of viable cells in a sample.
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Lab products found in correlation
3 protocols using celltitre glo cell viability assay
1
Assessment of PLA2G7 Silencing Effects
2
Doxycycline-Hydroxyurea Cell Viability Assay
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The cells were seeded in 96-well plates at a density of 2500 cells per well over night. Next day (day 0), the medium was changed with a fresh medium with or without 2 μg/ml doxycycline. After 24 h of doxycycline treatment (day 1), the medium was changed again with fresh medium containing serially diluted hydroxyurea with or without doxycycline. At day 4, the cell viability was assessed using the CellTitre-Glo cell viability assay kit (Promega, Cat. # G7572) using PHERAstar FSX (BMG Labtech, San Diego, California).
3
Knockdown of EZH2 in Leukemia Cell Lines
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Two shRNA encoding oligonucleotides targeting EZH2 (shEZH2_1: 5' -GAGAGATTATTTCTCAAGATG-3'; shEZH2_2: 5' -TGGAAAGAACGGAAATCTTAA -3'), or a non-targeting control shRNA (Sigma-Aldrich, St. Louis, MO, USA) were inserted between AgeI and EcoRI restriction sites into plasmid pLKO.1 (Sigma).
Expression constructs were transduced and selected in cell lines F-36P (ACC-543), MOLM-13 (ACC-554) and OCI-M2 (ACC-619), purchased from The Leibniz Institute DSMZ -German Collection of Microorganisms and Cell Cultures GmbH, as described previously 16 and tested for mycoplasma contamination. Growth curves of cell lines were generated using the CellTitre-Glo cell viability assay (PROMEGA, Fitchburg, WI, USA), according to manufacturer instructions.
Expression constructs were transduced and selected in cell lines F-36P (ACC-543), MOLM-13 (ACC-554) and OCI-M2 (ACC-619), purchased from The Leibniz Institute DSMZ -German Collection of Microorganisms and Cell Cultures GmbH, as described previously 16 and tested for mycoplasma contamination. Growth curves of cell lines were generated using the CellTitre-Glo cell viability assay (PROMEGA, Fitchburg, WI, USA), according to manufacturer instructions.
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