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Rosamt mg reporter mice

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The ROSAmT/mG reporter mice are genetically engineered mice that express a membrane-targeted version of the Tomato (mT) and membrane-targeted version of the Green Fluorescent Protein (mG) in a Cre-dependent manner. These mice can be used to visualize cell lineages and track the fate of specific cell populations in vivo.

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Lab products found in correlation

Plzf cre recombinase mice, by Jackson ImmunoResearch (2 mentions) R26 creert2 mice, by Jackson ImmunoResearch (2 mentions) Tamoxifen, by Merck Group (1 mentions) Myh11 cre, by Jackson ImmunoResearch (1 mentions) Myh11 creert2, by Jackson ImmunoResearch (1 mentions) Tdtomato reporter mice, by Jackson ImmunoResearch (1 mentions) Rag1 knockout mice, by Jackson ImmunoResearch (1 mentions) Batffl flplzf cre, by Jackson ImmunoResearch (1 mentions) C57bl 6 mice, by Jackson ImmunoResearch (1 mentions)

8 protocols using rosamt mg reporter mice

1

Targeting Mandibular Chondrocytes with Agc1-CreER

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To determine whether Agc1-CreER mice could target mandibular condylar articular chondrocytes efficiently in adult mice, Agc1-CreER transgenic mice were bred with ROSAmT/mG reporter mice (Muzumdar et al., 2007 (link)) (obtained from Jackson Laboratories). Tamoxifen was administered into 5-week-old mice. Mice were sacrificed at age of 9 weeks. Histologic sections were analyzed using a fluorescence microscope.
Liao L., Zhang S., Zhou G.Q., Ye L., Huang J., Zhao L, & chen D. (2018). Deletion of Runx2 in Condylar Chondrocytes Disrupts TMJ Tissue Homeostasis. Journal of cellular physiology, 234(4), 3436-3444.
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2

Influenza Infection in Conditional Knockout Mice

Cited 2 times
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Six to eight-week-old female C57BL/6 mice were obtained through the National Cancer Institute grantees program (Frederick, MD). Mice were bred and maintained in a closed breeding facility, and mouse handling conformed to the requirements of the Institutional Animal Care and Use Guidelines of Medical College of Wisconsin. Batffl/fl mice was previously described (70 (link)) and PLZF-Cre recombinase mice (stock #:024529) was purchased from Jackson and crossed to one another to generate either Batffl/flPLZF-Cre– or Batffl/flPLZF-Cre+ mice. Mice were infected with influenza A/PR8/34 (~50 PFU/mouse) to establish acute sublethal infection. Infection were performed by intranasal (i.n.) under anesthesia as described before (71 (link)). R26-CreERT2 mice (stock #: 008463) and RosamTmG reporter mice (stock #: 007576) were purchased from Jackson laboratory. Batffl/fl mice were crossed with ERT2CreRosamTmG mice to generate Batffl/flERT2CreRosamTmG mice.
Wu X., Kasmani M.Y., Zheng S., Khatun A., Chen Y., Winkler W., Zander R., Burns R., Taparowsky E.J., Sun J, & Cui W. (2022). BATF Promotes Group 2 Innate Lymphoid Cell-Mediated Lung Tissue Protection During Acute Respiratory Virus Infection. Science immunology, 7(67), eabc9934.
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3

Efficient Articular Chondrocyte Targeting

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To determine whether Agc1-CreER mice could target articular chondrocytes efficiently in adult mice, Agc1-CreER transgenic mice were bred with ROSAmT/mG reporter mice32 (link) (obtained from Jackson Laboratories). Tamoxifen was administered into 2-month-old mice. Mice were sacrificed at age of 3 months. Histologic sections were analyzed using a fluorescence microscope.
Liao L., Zhang S., Gu J., Takarada T., Yoneda Y., Huang J., Zhao L., Oh C.D., Li J., Wang B., Wang M, & Chen D. (2017). Deletion of Runx2 in Articular Chondrocytes Decelerates the Progression of DMM-Induced Osteoarthritis in Adult Mice. Scientific Reports, 7, 2371.
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4

Tspan12 Conditional Knockout Mice

Cited 1 time
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Tspan12 floxed (Tspan12tm1.1Hjug) and null (Tspan12tm1.2Hjug) alleles were reported previously and maintained on a C57BL/6J background.28 (link) Rosa mT/mG reporter mice were obtained from Jackson Laboratories. Recombination of Tspan12fl/-; Cre+ mice was induced at P28 with four intraperitoneal injections of 100 μL tamoxifen in cornoil (20 mg/ml) every 2 days. Tg(Cdh5-cre/ERT2)1Rha60 (link) was used as Cre driver. Animals of both sexes were used for all studies. Mice from P6 to nine month old were used, ages of mice are detailed in each figure. Mice were housed in a specific pathogen-free animal facility. All animal protocols were approved by the Animal Care and Use Committee of the University of Minnesota, Twin Cities.
Zhang L., Abedin M., Jo H.N., Levey J., Dinh Q.C., Chen Z., Angers S, & Junge H.J. (2023). A Frizzled4-LRP5 agonist promotes blood-retina barrier function by inducing a Norrin-like transcriptional response. iScience, 26(8), 107415.
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5

Genetic Manipulation of Cartilage Remodeling

Cited 6 times
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RosamT/mG reporter mice, Mmp13flox/flox mice, and Adamts5−/− mice were obtained from Jackson Laboratories (Bar Harbor, ME, USA) (Muzumdar et al., 2007 (link); Stickens et al., 2004 (link); McCulloch et al., 2009a (link), 2009b (link)). Co12CreERT2 transgenic mice were generated as previously described (Chen et al., 2007 (link); Zhu et al., 2008 (link); Jin et al., 2011 (link); Wang et al., 2012 (link); 2013 (link)). β-catenin(ex3)flox/flox mice were originally reported by Harada et al (1999) (link), and we have used these mice in our previous studies (Zhu et al., 2009 (link); Wang et al., 2012 (link), 2014 (link)). β-catenin(ex3)Co12ER mice, (β-catenin(ex3)/Mmp13) co12ER and β-catenin(ex3)Co12ER/Adamts5−/− double mutant mice, and their Cre-negative littermate control mice were generated as shown in Tables 1–3. Tamoxifen (Sigma, St. Louis, MO, USA) was administered into 2-week-old mice by intraperitoneal (i.p.) injection (1 mg/l0 g body weight for 5 d), n = 5 in each group. Animal protocols were approved by the IACUC of the University of Rochester.
Wang M., Li S., Xie W., Shen J., Im H.J., Holz J.D., Wang M., Diekwisch T.G, & Chen D. (2014). ACTIVATION OF B-CATENIN SIGNALLING LEADS TO TEMPOROMANDIBULAR JOINT DEFECTS. European cells & materials, 28, 223-235.
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6

UCP1-TRAP Mice for Thermogenesis

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Animal experiments were performed according to procedures approved by the Dana-Farber Cancer Institute IACUC. Myh11-Cre (stock #007742), Myh11-Cre/ERT2 (stock #019079), tdTomato reporter mice (stock #007914), and ROSAmT/mG reporter mice (stock #007676) were obtained from Jackson Laboratories. The generation of TRAP and Ucp1-Cre transgenic mice is described in detail in the Supplemental Experimental Procedures. UCP1-TRAP breeding pairs were housed at 30°C and litters were weaned to room temperature at 3 weeks. Unless otherwise stated, for cold exposure experiments 4 week-old female UCP1-TRAP mice were individually housed at 4°C for two weeks.
Long J.Z., Svensson K.J., Tsai L., Zeng X., Roh H.C., Kong X., Rao R.R., Lou J., Lokurkar I., Baur W., Castellot JJ J.r., Rosen E.D, & Spiegelman B.M. (2014). Ribosomal Profiling Provides Evidence for a Smooth Muscle-Like Origin of Beige Adipocytes. Cell metabolism, 19(5), 810-820.
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7

Generation of Batf Conditional Knockout Mice

Cited 1 time
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Batffl/fl mice were kindly provided by E.J. Taparowsky (Betz et al., 2010 (link)). PLZF-Cre recombinase mice (stock #:024529) were purchased from the Jackson Laboratory and crossed to one another to generate age-matched Batffl/flPLZF-Cre− (WT) and Batffl/flPLZF-Cre+ (Batf innate lymphoid cell-conditional knockout, cKO) mice. Batf knockout mice (stock #: 013758) and Rag1 knockout mice (stock #:129S7) were purchased from Jackson Laboratory. Rag1−/−Batf−/− mice were generated by crossing Rag1−/− with Batf−/− mice. R26-CreERT2 mice (stock #: 008463) and RosamT/mG reporter mice (stock #: 007576) were purchased from the Jackson laboratory. Batffl/fl was crossed with ERT2CreRosamTmG to generate Batffl/flERT2CreRosamTmG mice. Mice were bred and maintained in a closed breeding facility, and mouse handling conformed to the requirements of the Institutional Animal Care and Use Guidelines of the Medical College of Wisconsin.
Wu X., Khatun A., Kasmani M.Y., Chen Y., Zheng S., Atkinson S., Nguyen C., Burns R., Taparowsky E.J., Salzman N.H., Hand T.W, & Cui W. (2022). Group 3 innate lymphoid cells require BATF to regulate gut homeostasis in mice. The Journal of Experimental Medicine, 219(11), e20211861.
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8

Conditional Knockout of Socs3 in Nestin-Expressing Cells

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All animal studies were approved by the Institutional Animal Care and Use Committee at the Boston Children’s Hospital. Nestin Cre expressing C57Bl/6 mice (Jackson Laboratory, stock # 003771) were crossed with Socs3 flox/flox (Socs3 f/f) mice (kind gift of Dr. A. Yoshimura) to generate Socs3 cKO and littermate control flox mice. ROSAmTmG reporter mice were from the Jackson Laboratory (stock# 007576). C57Bl/6 mice were from the Jackson Laboratory (stock # 000664).
Sun Y., Ju M., Lin Z., Fredrick T.W., Evans L.P., Tian K.T., Saba N.J., Morss P.C., Pu W.T., Chen J., Stahl A., Joyal J.S, & Smith L.E. (2015). SOCS3 in retinal neurons and glial cells suppresses VEGF signaling to prevent pathological neovascular growth. Science signaling, 8(395), ra94.
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