Phospho p44 42mapk erk1 2 rabbit mab

SDS-PAGE and Western blot analyses used the following primary antibodies. The reents were purchased as follows: Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) (14C10) Rabbit mAb (cat. #2118, Cell Signaling, Beverly, MA, USA), Phospho-p44/42MAPK (Erk1/2) Rabbit mAb (cat. #4370, Cell Signaling, USA), p44/42 MAPK (Erk1/2) Rabbit mAb (cat. #4695, Cell Signaling, USA), CREB (48H2) Rabbit mAb (cat. #9197, Cell Signaling, USA), Purified Mouse Anti-Arc (cat. #612602, San Jose, CA, USA), and Rabbit monoclonal [E113] to CREB (phospho S133; cat. #ab32096, Abcam, Cambridge, MA, USA).
The results were scanned into the computer, and we used Image J software to analyze the gray value of the protein strip.

Bradykinin (SC090), culture and reagents were all purchased from Sigma-Aldrich (MO, St Louis, USA). DC Protein^TM assay, electrophoresis reagents and Clarity^TM Western ECL were purchased from Bio-Rad (Hercules, California, USA). PD 98,059 and Ibuprofen were ordered from Cayman Co (MI, Ann Arbor, USA). Antibodies used as follow: Anti-β-Actin (Abcam, Cambridge, UK, ab8227), Phospho-p44/42 MAPK (ERK1/2) rabbit mAb (Cell Signaling Technology, Danvers, MA, USA, 4370 s), p42/44 MAPK rabbit mAb (ERK1/2) (Cell Signaling Technology, 4695 s), Peroxidase-conjugated affinipure donkey anti-rabbit IgG (Jackson ImmunoResearch, West Grove, PA, USA) 711-035-152), or affinipure donkey anti-mouse IgG (Jackson Immune, 715–035-150), mouse monoclonal antibody anti-COX-2 (clone COX214). HPLC water was purchased from Avantor (Valley Center, PA, USA), acetonitrile (ACN) from Thermo Fisher Scientific (Waltham, MA, USA), trypsin/Lys-C mixture was produced by Promega (Madison, WI, United States) and formic acid (FA) was obtained from (TCI America, Portland, OR, USA).