Cgp 55845 hydrochloride

Manufactured by Bio-Techne

Sourced in United Kingdom

CGP 55845 hydrochloride is a pharmaceutical agent that functions as a potent and selective antagonist of GABAB receptors. It is commonly used as a research tool in the study of GABAB receptor physiology and pharmacology.

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Lab products found in correlation

D ap5, by Bio-Techne (3 mentions) Qx 314, by Bio-Techne (2 mentions) Bicuculline methobromide, by Bio-Techne (2 mentions) Way100635 maleate, by Bio-Techne (1 mentions) Dpcpx, by Bio-Techne (1 mentions) 4 aminopyridine 4 ap, by Merck Group (1 mentions) Picrotoxin, by Bio-Techne (1 mentions) L 689 560, by Bio-Techne (1 mentions) Dithiothreitol (dtt), by Merck Group (1 mentions) Tetrodotoxin citrate, by Bio-Techne (1 mentions) Mrs1523, by Merck Group (1 mentions) Milli q system, by Merck Group (1 mentions) Ana 12, by Bio-Techne (1 mentions) α bungarotoxin α bgt, by Abcam (1 mentions) Apv d 2 amino 5 phosphonopentanoic acid, by Merck Group (1 mentions) Gentamicin, by Thermo Fisher Scientific (1 mentions) Penicillin, by Thermo Fisher Scientific (1 mentions) Streptomycin, by Thermo Fisher Scientific (1 mentions) Carboxymethylcellulose, by Merck Group (1 mentions) Baclofen, by Merck Group (1 mentions)

8 protocols using cgp 55845 hydrochloride

Norepinephrine Modulation of Synaptic Transmission

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Drugs were added to the external ACSF. NE was bath-applied for 5 min and reached its maximal effect around 3 min after application. Slices were pre-incubated with prazosin for 15 to 20 min. All substances were obtained from Sigma-Aldrich (Diesenhofen, Germany), except for DNQX, AP5 and CGP55845 hydrochloride (Tocris, Bristol, UK).

Meis S., Endres T., Munsch T, & Lessmann V. (2019). Impact of Chronic BDNF Depletion on GABAergic Synaptic Transmission in the Lateral Amygdala. International Journal of Molecular Sciences, 20(17), 4310.

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Trazodone Sourcing and Compounds

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Trazodone was provided by A.C.R.A.F. S.p.A. (Santa Palomba, Rome). 5-HT, SR-95531, d-AP5, NBQX were purchased from Ascent Scientific Ltd. (Bristol, UK). Way-100635 maleate, CGP-55845 hydrochloride, 5-CT and DPCPX were purchased from Tocris Bioscience (Bristol, UK). L-Phenylephrine and strychnine hydrochloride from Sigma-Aldrich S.r.l. (Milan, Italy).

Montalbano A., Mlinar B., Bonfiglio F., Polenzani L., Magnani M, & Corradetti R. (2019). Dual inhibitory action of trazodone on dorsal raphe serotonergic neurons through 5-HT1A receptor partial agonism and α1-adrenoceptor antagonism. PLoS ONE, 14(9), e0222855.

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Pharmacological Agents for Neurophysiology

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Bicuculline methobromide, CGP55845 hydrochloride, D-AP5, CNQX, QX314, and tetraethylammonium chloride (TEA) were purchased from Tocris Bioscience (Bristol, UK). 4-Aminopyridine (4-AP) was purchased from Sigma-Aldrich (St. Louis, MO, USA).

Choi J.H., Yarishkin O., Kim E., Bae Y., Kim A., Kim S.C., Ryoo K., Cho C.H., Hwang E.M, & Park J.Y. (2018). TWIK-1/TASK-3 heterodimeric channels contribute to the neurotensin-mediated excitation of hippocampal dentate gyrus granule cells. Experimental & Molecular Medicine, 50(11), 145.

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Compound Dissolution and Preparation

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L-689,560, Picrotoxin, NBQX, D-AP5, and CGP55845 hydrochloride were purchased from Tocris, UK. Compounds were dissolved in DMSO, except Picrotoxin and D-AP5, which were dissolved in water. Compounds were separated into appropriate aliquots and stored at −20°C.
GTPɣ[³⁵S] and Anti-rabbit SPA beads were purchased from PerkinElmer, UK, Gqα antibody (E17) was from Santa Cruz Biotechnology, Nonidet P-40 10% solution from Roche Applied Sciences, Dithiothreitol (DTT) from Sigma, UK, and Complete protease inhibitor cocktail purchased from Roche Applied Sciences
GSK-5 (Compound 5 from [Budzik et al. 2010 (link)]) and 77-LH-28-1 (1-[3-(4-butyl-1-piperidinyl)propyl]-3,4-dihydro-2[1H]-quinolinone) were synthesized in-house at Eli Lilly and Company Ltd.

Dennis S.H., Pasqui F., Colvin E.M., Sanger H., Mogg A.J., Felder C.C., Broad L.M., Fitzjohn S.M., Isaac J.T, & Mellor J.R. (2015). Activation of Muscarinic M1 Acetylcholine Receptors Induces Long-Term Potentiation in the Hippocampus. Cerebral Cortex (New York, NY), 26(1), 414-426.

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Neurochemical Modulators in Cell Signaling

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CXCL16 (murine, Peprotech Inc. Rocky Hill, NJ, USA), Tetrodotoxin citrate (Tocris, Bristol, UK, stock solution 1 mM in ethanol), CGP 55845 hydrochloride (Tocris, Bristol, UK, stock solution 10 mM in DMSO), MRS 1523 (Sigma Aldrich, Saint Louis, Missouri, USA, stock solution 10 mM in DMSO). Ultrapure water was prepared using a Milli-Q system (Millipore, MA, USA). Drugs were dissolved in ACSF just before application.

Di Castro M.A., Trettel F., Milior G., Maggi L., Ragozzino D, & Limatola C. (2016). The chemokine CXCL16 modulates neurotransmitter release in hippocampal CA1 area. Scientific Reports, 6, 34633.

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Neurotransmitter Receptor Antagonists

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Bicuculline methobromide, CGP 55845 hydrochloride, D-AP5, CNQX, and QX-314 were purchased from Tocris. Other reagents were purchased from Sigma or from Calbiochem.

Yarishkin O., Lee D.Y., Kim E., Cho C.H., Choi J.H., Lee C.J., Hwang E.M, & Park J.Y. (2014). TWIK-1 contributes to the intrinsic excitability of dentate granule cells in mouse hippocampus. Molecular Brain, 7, 80.

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Production and Purification of sAPPα and sAPPβ

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CGP 55845 hydrochloride (Tocris, #1248), ±α-Methyl-4-carboxyphenylglycine (Sigma, #M-4796), ANA-12 (Tocris, #4781), D(−)-2-Amino-5-phosphonopentanoic acid (APV; Sigma, #A8054), α-Bungarotoxin (αBGT; Abcam, # ab120542).
sAPPα and sAPPβ production and purification was carried out according to the protocols developed by the Tate lab (Turner et al., 2007 (link)).

Livingstone R.W., Elder M.K., Barrett M.C., Westlake C.M., Peppercorn K., Tate W.P., Abraham W.C, & Williams J.M. (2019). Secreted Amyloid Precursor Protein-Alpha Promotes Arc Protein Synthesis in Hippocampal Neurons. Frontiers in Molecular Neuroscience, 12, 198.

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Reagents and Materials for Cell Experiments

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Fetal bovine serum (FBS), acetylcholine chloride (ACh), ( ± )-baclofen, and BAPTA-AM were obtained from Sigma-Aldrich (St. Louis, MO, USA), whereas glucose and carboxymethyl cellulose were obtained from Sigma-Aldrich SRL (Milan, Italy). Oxaliplatin, was obtained from Carbosynth (Berkshire, UK). Gentamicin was obtained from Gibco (Grand Island, NY, USA). DMEM, GlutaMAX, penicillin, and streptomycin were purchased from Invitrogen Life Technologies (Carlsbad, CA, USA). Fetal bovine serum (FBS) for cell culture was obtained from Bovogen (East Keilor, VIC, Australia). Collagenase Type II was purchased from Worthington Biochemical Corp. (Lakewood, NJ, USA). CGP 55845 hydrochloride was purchased from Tocris Bioscience (Bristol, UK). ( ± )-18-Methoxycoronaridine hydrochloride [( ± )-18-MC] was purchased from Obiter Research, LLC (Champaign, IL, USA). (+)-Catharanthine (base) was obtained from Cayman Chemicals (Ann Arbor, MI, USA). (+)-Catharanthine hydrochloride was a gift from Dr. Kuehne (University of Vermont, VT, USA). Salts were of analytical grade.

Arias H.R., Tae H.S., Micheli L., Yousuf A., Ghelardini C., Adams D.J, & Di Cesare Mannelli L. (2020). Coronaridine congeners decrease neuropathic pain in mice and inhibit α9α10 nicotinic acetylcholine receptors and Ca_V2.2 channels. Neuropharmacology, 175.

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