Anti gfp antibody 632380

Anti-Set8 (C18B7; 1:1,000 immunoblot (IB)), anti-β-catenin (D10A8; 1:1,000 IB), anti-Skp2 (D3G5; 1:1,000 IB), anti-Mcl-1 (D35A5; 1:1,000 IB), anti-G9A (C6H3; 1:1,000 IB), anti-Cullin 1 (4995; 1:1,000 IB) and anti-β-TRCP1 (4394; 1:1,000 IB) antibodies were purchased from Cell Signaling Technology. Anti-p27 (SC-528; 1:500 IB), anti-Cullin 1 (SC-17775; 1:1,000 IB), anti-casein kinase 1δ (H-60; 1:1,000 IB), anti-casein kinase 1α (SC-6474; 1:1,000 IB), anti-c-Myc (9E10; 1:1,000 IB, 2 μg immunoprecipitates (IP)) and polyclonal anti-HA antibodies (SC-805; 1:1,000 IB, 2 μg IP) were purchased from Santa Cruz Biotechnology. Anti-Vinculin antibody (V-4505; 1:2,000 IB), monoclonal anti-Flag antibody (F-3165), polyclonal anti-Flag antibody (F-2425; 1:1,000 IB, 2 μg IP), anti-HA agarose beads (A-2095; 1:1,000 IB, 2 μg IP), peroxidase-conjugated anti-mouse secondary antibody (A-4416; 1:3,000 IB) and peroxidase-conjugated anti-rabbit secondary antibody (A-4914; 1:3,000 IB) were purchased from Sigma. Monoclonal anti-HA antibody (MMS-101P) was purchased from Covance. Anti-GFP antibody (632380; 1:1,000 IB), anti-Cdh1 (34-2000; 1:1,000 IB) antibody, Oligofectamine, Lipofectamine and Plus reagents were purchased from Invitrogen. Anti-CUL 4B (16184-1-AP; 1:1,000 IB) antibody was purchased from Proteintech Group.

Anti-NEDD4 (4013), anti-PTEN (9188), anti-Akt1 (2938), anti-pAkt (S473) (9018), anti-pAkt (T308) (13038), anti-p-FOXO3a (T32) (9464), anti-FOXO3a (2497) and anti-Cdc25A (3652) antibodies were purchased from Cell Signaling Technology. Anti-c-Myc (9E10) and polyclonal anti-HA antibodies (SC-805) were purchased from Santa Cruz Biotechnology. Anti-Vinculin antibody (V-4505), polyclonal anti-Flag antibody (F-2425), monoclonal anti-Flag antibody (F-3165), anti-HA agarose beads (A-2095), peroxidase-conjugated anti-mouse secondary antibody (A-4416) and peroxidase-conjugated anti-rabbit secondary antibody (A-4914) were purchased from Sigma. Anti-GFP antibody (632380) was obtained from Invitrogen. Anti-Cullin 1 (4995) and anti–β-TRCP1 (4394) antibodies were purchased from Cell Signaling Technology. Monoclonal anti-HA antibody (MMS-101P) was purchased from Covance. Oligofectamine, Lipofectamine and Plus reagents were purchased from Life Technologies.