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Sodium pyruvate

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Sodium pyruvate is a chemical compound that serves as a source of the pyruvate anion. It is a white, crystalline solid that is soluble in water and other polar solvents. Sodium pyruvate is commonly used in cell culture media and as a biochemical reagent.

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Lab products found in correlation

Penicillin streptomycin, by Thermo Fisher Scientific (3 mentions) Hl 60 cells, by American Type Culture Collection (2 mentions) Rpmi 1640 media, by Cambrex (2 mentions) Iscove modified dulbecco media (imdm), by American Type Culture Collection (2 mentions) Fetal bovine serum (fbs), by Merck Group (2 mentions) 7 aad staining, by Thermo Fisher Scientific (1 mentions) Penicillin, by Cambrex (1 mentions)

4 protocols using sodium pyruvate

1

Isolation and Stimulation of PBMCs

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Peripheral mononuclear blood cells (PBMCs) were isolated by Ficoll density gradient centrifugation immediately after collecting the blood samples. Isolated PBMCs were washed, counted and incubated at a concentration of 1x106 cells/mL in 12-well plates at 37°C and 5% CO2. Isolated B-cells were cultured in 96-well flat bottom plates. Cell culture was performed in complete RPMI-1640 media containing 10% fetal calf serum, 2mM L-glutamine, 100 U/ml penicillin/streptomycin (1%, all from Invitrogen), 1% sodium bicarbonate and 1% sodium pyruvate (Cambrex). PBMCs were stimulated with 1μM ODN2006 Type B CPG (Invivogen, San Diego, USA) per well or were left unstimulated for control purposes and incubated for 72 hours. For the last six hours 2μl/mL cell stimulation cocktail plus protein transport inhibitors 500x containing PMA (40.5μM), Ionomycin (670μM), the protein transport inhibitors Brefeldin A (5.3mM), and Monensin (1mM) was added. Viability of cells before and after cell culture was assessed using 7-AAD staining (eBioscience) and methylen-blue.
Tebbe B., Wilde B., Ye Z., Wang J., Wang X., Jian F., Dolff S., Schedlowski M., Hoyer P.F., Kribben A., Witzke O, & Hoerning A. (2016). Renal Transplant Recipients Treated with Calcineurin-Inhibitors Lack Circulating Immature Transitional CD19+CD24hiCD38hi Regulatory B-Lymphocytes. PLoS ONE, 11(4), e0153170.
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2

Neutrophil-like Differentiation of HL-60 Cells

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HL-60 cells (CCL-240, ATCC, Manassas, VA) were cultured at 37°C in 5% CO2 in Iscove's Modified Dulbecco's Medium (IMDM, ATCC, Manassas, VA) containing 20% FBS (Sigma-Aldrich, St. Louis, MO) and differentiated to neutrophil-like cells by DMSO-induced differentiation for a period of 5 days. Cells were sub-cultured every third day to a density of 1×106 cells mL−1. During migration assays, neutrophils were suspended in a buffer of 0.2% human serum albumin (HSA, Sigma-Aldrich) in Hanks Buffered Salt Solution (HBSS, ATCC, Manassas,VA). T-lymphocytes were suspended in RPMI 1640 media (Cambrex, Charles City, IA) containing 10% fetal bovine serum, 2 mM L-glutamine, 100 U mL−1 penicillin/streptomycin (Gibco-Invitrogen), 1% sodium bicarbonate and 1% sodium pyruvate (Cambrex).
Boneschansker L., Yan J., Wong E., Briscoe D.M, & Irimia D. (2014). MICROFLUIDIC PLATFORM FOR THE QUANTITATIVE ANALYSIS OF LEUKOCYTE MIGRATION SIGNATURES. Nature communications, 5, 4787.
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3

Neutrophil-like Differentiation of HL-60 Cells

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HL-60 cells (CCL-240, ATCC, Manassas, VA) were cultured at 37°C in 5% CO2 in Iscove's Modified Dulbecco's Medium (IMDM, ATCC, Manassas, VA) containing 20% FBS (Sigma-Aldrich, St. Louis, MO) and differentiated to neutrophil-like cells by DMSO-induced differentiation for a period of 5 days. Cells were sub-cultured every third day to a density of 1×106 cells mL−1. During migration assays, neutrophils were suspended in a buffer of 0.2% human serum albumin (HSA, Sigma-Aldrich) in Hanks Buffered Salt Solution (HBSS, ATCC, Manassas,VA). T-lymphocytes were suspended in RPMI 1640 media (Cambrex, Charles City, IA) containing 10% fetal bovine serum, 2 mM L-glutamine, 100 U mL−1 penicillin/streptomycin (Gibco-Invitrogen), 1% sodium bicarbonate and 1% sodium pyruvate (Cambrex).
Boneschansker L., Yan J., Wong E., Briscoe D.M, & Irimia D. (2014). MICROFLUIDIC PLATFORM FOR THE QUANTITATIVE ANALYSIS OF LEUKOCYTE MIGRATION SIGNATURES. Nature communications, 5, 4787.
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4

Expansion of Primary Vγ9Vδ2 T Cells

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Primary Vγ9Vδ2 T cell cultures were generated from peripheral blood mononuclear cells (PBMCs) isolated from blood of healthy donors (Etablissement Français du Sang, Toulouse, France). Briefly, PBMC were stimulated with BrHPP (3 μM) and rhIL-2 (300 IU/ml) in complete RPMI 1640 culture medium (Invitrogen, Cergy Pontoise, France) supplemented with 10% fetal calf serum (Hy1, Thermo Scientific, USA), 100 g/ml streptomycin, 100 IU/ml penicillin and 1 mM sodium-pyruvate (Cambrex Biosciences, Rockland, ME, USA) for 14 days. Purity of the Vγ9Vδ2 T cells was >95% as determined by flow cytometry using an anti-TCRVγ9Vδ2 mAb.
Laplagne C., Meddour S., Figarol S., Michelas M., Calvayrac O., Favre G., Laurent C., Fournié J.J., Cabantous S, & Poupot M. (2020). Vγ9Vδ2 T Cells Activation Through Phosphoantigens Can Be Impaired by a RHOB Rerouting in Lung Cancer. Frontiers in Immunology, 11, 1396.
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