AAV1-CaMKII-Cre was obtained from the University of Pennsylvania Viral Vector Core (Cat#AV-1-PV2396). AAV1-CAG-FLEX-GFP and AAV1-CAG-FLEX-tdTomato were obtained from Addgene (#51502-AAV1, #51503-AAV1). pAAV-FLEX-GFP (Addgene #28304) and pAAV-FLEX-tdTomato (Addgene #28306) were gifts from Edward Boyden (Massachusetts Institute of Technology). AAVDJ/8-CAG-FLEX-tdTomato and AAVDJ/8-CAG-FLEX-GFP were produced with pAAV2-1 and purified as described previously [38 (link), 39 (link)].
Aav1 cag flex tdtomato
Manufactured by Addgene
Sourced in United States
AAV1-CAG-FLEX-tdTomato is an adeno-associated virus (AAV) vector that contains the tdTomato fluorescent protein gene under the control of a constitutive CAG promoter. The FLEX (Flip-Excision) system allows for Cre-dependent expression of the tdTomato gene.
Automatically generated - may contain errors
Lab products found in correlation
Paav flex gfp, by Addgene (1 mentions)
Paav flex tdtomato, by Addgene (1 mentions)
Aavrg hsyn cre wpre hgh, by Addgene (1 mentions)
Aav9 hsyn dio hm4d gi mcherry, by Addgene (1 mentions)
Aav8 hsyn dio hm3d gq mcherry, by Addgene (1 mentions)
Aav cag gfp, by Addgene (1 mentions)
Aav syn gfp, by Addgene (1 mentions)
3 protocols using aav1 cag flex tdtomato
1
Versatile AAV Viral Vector Constructs
2
Optogenetics and Viral Tracing Tools
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
AAV1-EF1α-double floxed-hChR2(H134R)-EYFP-WPRE-HGHpA, AAV1-Ef1α-DIO-EYFP, AAV1-Syn-FLEX-GCaMP6s, AAV1-Syn-FLEX-GCaMP6f, AAV8-hSyn-DIO-hM3D(Gq)-mCherry, AAV9-hSyn-DIO-hM4D(Gi)-mCherry, AAVrg-hSyn-Cre-WPRE-hGH, AAV9-CamKIIa-ChR2-eYFP, AAV1-CAG-FLEX-tdTomato, AAV1-mDlx-NLS-mRuby2 were obtained from Addgene. AAV1-EF1a-FLEX-TVA-mCherry from UNC vector core, AAV1-FLEX-2A-G(N2C)-mKate and RABV-ΔG -GFP-EnvA, a gift from Charles Zuker.
3
Twelve AAV Vectors for Oxytocin and Vasopressin Neurons
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
We tested twelve adeno associated viral vectors (AAV) for their ability to transduce oxytocin and arginine-vasopressin neurons in the PVN: AAV-CAG-GFP and AAV-SYN-GFP, serotypes 1 and 9 (catalog numbers: 37825 and 50465, respectively, Addgene, Cambridge, MA, USA); AAVDJ-SYN-GFP and AAVDJ-Ef1α-EYFP (catalog numbers: GVVC-AAV-127 and GVVC-AAV-168, respectively, Stanford Viral and Vector core, Stanford, CA, USA); AAV1-CAG-FLEX-tdTomato and AAV1-SYN-HI-eGFP-CRE-WPRE-SV40 (catalog numbers: 28306 and 105540, respectively, Addgene, Cambridge, MA, USA); AAV1/2-OXTp-Venus, AAV1/2-OXTp-Ires-CRE and AAV1/2-SYN-tdTomato and AAV1/2-OXTp-GCaMP6s produced by Dr. Valery Grinevich’s lab at the University Heidelberg, Germany. Viral titers are detailed in Tables 1 and 2 .
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!