Nb100 68252

Manufactured by Abcam

NB100–68252 is a laboratory equipment product. It is a device designed for use in scientific research and experimentation. The core function of this product is to provide a specific capability or tool for laboratory work. No further details on the intended use or specific features of this product can be provided in an unbiased and factual manner.

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Lab products found in correlation

Anti actin, by Merck Group (1 mentions) Mab1501, by Merck Group (1 mentions) Anti ha, by Cell Signaling Technology (1 mentions) Anti flag, by Proteintech (1 mentions) Ab157106, by Proteintech (1 mentions) Anti naf1, by Abcam (1 mentions) Anti nhp2, by Proteintech (1 mentions) Anti nop10, by Abcam (1 mentions) Anti htert, by Santa Cruz Biotechnology (1 mentions) Anti dyskerin, by Santa Cruz Biotechnology (1 mentions) Anti tip60, by Santa Cruz Biotechnology (1 mentions) Mini protean tgx stain free gel, by Bio-Rad (1 mentions) Chemidoc mp, by Bio-Rad (1 mentions) Ab32020, by Abcam (1 mentions) Trans blot turbo transfer system, by Bio-Rad (1 mentions) Nb300 269, by Novus Biologicals (1 mentions)

2 protocols using nb100 68252

Protein Expression and IP Analysis

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Analysis of protein expression and IP experiments was performed by resolving proteins by SDS-PAGE, transfer to PVDF and immunoblotting. Primary antibodies used for immunoblotting were: anti-FLAG (Proteintech, 20543-1-AP, 1:4000), anti-HA (Cell Signaling, 2367, 6E2, 1:1125), anti-NAF1 (Abcam, ab157106, 1:1000), anti-NHP2 (Proteintech, 15128–1-AP, 1:5000), anti-NOP10 (Abcam, ab134902, 1:500), anti-TCAB1 (Novus, NB100–68252, 1:2000), anti-reptin (Abcam, ab51500, 1:5000), anti-hTERT (Santa Cruz, sc7215, C-20, 1:500), anti-dyskerin (Santa Cruz, sc-373956, H-3, 1:1500), anti-PARN (Abcam, ab154214, 1:500), anti-RRP40 (Bethyl, A303–909A-T, 1/1500), anti-TIP60 (Santa Cruz, sc5725, N-17, 1/1000), anti-hGAR1 (from Dr Witold Filipowicz (40 (link)), 1/2000) and anti-actin (Chemicon MAB1501, 1:5000).

MacNeil D.E., Lambert-Lanteigne P, & Autexier C. (2019). N-terminal residues of human dyskerin are required for interactions with telomerase RNA that prevent RNA degradation. Nucleic Acids Research, 47(10), 5368-5380.

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Western Blot Analysis of Telomerase Proteins

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Mini-PROTEAN TGX stain-free gels (Bio-Rad) were used for SDS-PAGE. Total protein was detected using a ChemiDoc MP (Bio-Rad) after a 45 s UV activation. Western transfer was carried out using the Trans-Blot Turbo transfer system (Bio-Rad) according to the manufacturer’s instructions using the mixed molecular weight transfer setting. Immuno-blotting was carried out using standard protocols. The C-terminal TCAB1 antibody (Proteintech, 14761-1-AP) was used at a 1:2000 dilution, the N-terminal TCAB1 antibody (Novus Biologicals, NB100-68252) was used at a 1:1000 dilution, the TERT antibody (Abcam, ab32020) was used at a 1:4000 dilution, the dyskerin antibody (Santa Cruz Biotech, sc-373956) was used at a 1:200 dilution, the GAR1 antibody was used at a 1:2000 dilution (Proteintech, 11711-1-AP), the fibrillarin antibody was used at a 1:2000 dilution (Novus Biologicals, NB300-269), and the lamin B1 antibody was used a 1:2000 dilution. Secondary antibodies were used at a 1:5000 dilution.

Klump B.M., Perez G.I., Patrick E.M., Adams-Boone K., Cohen S.B., Han L., Yu K, & Schmidt J.C. (2023). TCAB1 prevents nucleolar accumulation of the telomerase RNA to facilitate telomerase assembly. Cell reports, 42(6), 112577.

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