Mouse monoclonal anti perk

Manufactured by Santa Cruz Biotechnology

Sourced in United States

Mouse monoclonal anti-pERK is a laboratory reagent used for the detection and quantification of phosphorylated extracellular signal-regulated kinase (pERK) in various research applications. This product is a purified mouse monoclonal antibody that specifically binds to the phosphorylated form of ERK, a key protein involved in cellular signaling pathways.

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Lab products found in correlation

Mouse monoclonal anti p53, by Santa Cruz Biotechnology (2 mentions) Anti neurod, by Santa Cruz Biotechnology (1 mentions) Anti p akt, by Cell Signaling Technology (1 mentions) Anti foxo1, by Cell Signaling Technology (1 mentions) Anti lamin b1, by Santa Cruz Biotechnology (1 mentions) Anti α tubulin, by Merck Group (1 mentions) Anti pi3k, by Cell Signaling Technology (1 mentions) Anti p pi3k, by Cell Signaling Technology (1 mentions) Anti par, by BD (1 mentions) Pierce protease and phosphatase inhibitor mini tablet, by Thermo Fisher Scientific (1 mentions) Goat anti rabbit igg hrp conjugate, by Bio-Rad (1 mentions) Goat anti mouse igg hrp conjugate, by Santa Cruz Biotechnology (1 mentions) Rabbit anti β actin polyclonal antibody, by Merck Group (1 mentions) Pierce ecl western blotting substrate, by Thermo Fisher Scientific (1 mentions) Goat anti mouse igg hrp, by Fortis Life Sciences (1 mentions) Goat anti rabbit igg hrp, by Fortis Life Sciences (1 mentions) Anti atf6, by Proteintech (1 mentions) Anti phospho eif2 alpha ser51, by Cell Signaling Technology (1 mentions) Anti xbp1, by Novus Biologicals (1 mentions) Anti chop gadd153, by Proteintech (1 mentions)

4 protocols using mouse monoclonal anti perk

Antibody Characterization in Cell Lines

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The following antibodies were used: mouse monoclonal anti-PARP-1, mouse monoclonal anti-BrdU (#347580), mouse monoclonal anti-p21 (#556431), mouse monoclonal anti-p27 (#610241), rabbit polyclonal anti-PAR (#551813,; BD Bioscience), mouse monoclonal anti-α-tubulin (#T5168, Sigma-Aldrich), mouse monoclonal antibodies for Myc (#G019), HA (#G036), Flag (#G191), and GFP (#G096, Abm), rabbit polyclonal anti-MAP2 (#MAB3418), anti-Olig2 (#ab9610), anti-SOX2 (#ab5603, Millipore), anti-GFAP (#ab7260), anti-Ki67 (#ab15580), anti-Tbr2 (#ab183991) and mouse monoclonal anti-Nestin (#ab22035, abcam), goat polyclonal anti-DCX (#sc-8066), anti-NeuroD (#sc-1084), rabbit polyclonal anti-ERK (#sc-93), mouse monoclonal anti-pERK (#sc-7383), anti-LaminB1 (#sc-56145, Santa Cruz), rabbit polyclonal anti-Akt (#4060), rabbit polyclonal anti-pAkt (#4061), rabbit polyclonal anti-PI3K (#4228), anti-pPI3K (#4257), rabbit polyclonal anti-FOXO1 (#2880), rabbit polyclonal anti-pFOXO1 antibody (#9461, Cell Signaling).

Hong S., Yi J.H., Lee S., Park C.H., Ryu J.H., Shin K.S, & Kang S.J. (2019). Defective neurogenesis and schizophrenia-like behavior in PARP-1-deficient mice. Cell Death & Disease, 10(12), 943.

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Western Blot Analysis of Cell Signaling

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After washing cells with PBS, whole cell protein was extracted by lysing cells with RIPA buffer (50 mM Tris-HCl (pH 7.4), 150 mM NaCl, 1 mM Na₂EDTA, 0.5% sodium deoxycholate, 1.0% Triton X-100, and 0.1% sodium dodecyl sulfate) supplemented with Pierce Protease and Phosphatase Inhibitor Mini Tablets (Thermo Scientific, #88668). Immunoblotting was performed as described previously [32 (link)]. The rabbit anti-total JNK1/2 polyclonal antibody (#9252) was from Cell Signaling Technology (Danvers, MA). The mouse monoclonal anti-p-ERK (#sc-7383), rabbit polyclonal anti-total ERK1/2 (#sc-94), and the rabbit polyclonal anti-cFOS antibodies were from Santa Cruz Biotechnology Inc. (Santa Cruz, CA) The rabbit anti-β-actin polyclonal antibody (#A2066) from Sigma-Aldrich. The rabbit polyclonal anti-ATF4 (#ABE387) was from EMD Millipore. A goat anti-rabbit IgG-HRP conjugate (BioRad, #170-6515) and a goat anti-mouse IgG-HRP conjugate (Santa Cruz, #sc-2005) were used as the secondary antibodies (BioRad, #170-6515). Bound secondary antibody was detected using Pierce ECL Western Blotting Substrate (Thermo Scientific, #32106) according to the manufacturer’s protocol followed by chemiluminescent imaging on autoradiography film.

Shan J., Donelan W., Hayner J.N., Zhang F., Dudenhausen E.E, & Kilberg M.S. (2014). MAPK signaling triggers transcriptional induction of cFOS during amino acid limitation of HepG2 cells. Biochimica et biophysica acta, 1854(3), 539-548.

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Western Blot Antibody Validation Protocol

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To evaluate protein expression on Western blot membranes, the following antibody were used: rabbit polyclonal anti-ATF6 (1:600) (Proteintech, 24169-1-AP), rabbit polyclonal anti-XBP1 (1:1000) (Novus Biologicals, NB100-80861), rabbit polyclonal anti-phospho-eIF2alpha (Ser51) (1:1000) (Cell Signaling, 9721), rabbit polyclonal anti-eIF2alpha (1:4000) (Cell Signaling, 9722), rabbit polyclonal anti-BiP/GRIP78 (1:5000) (Proteintech, 11587-1-AP), rabbit polyclonal anti-CHOP (GADD153) (1:1000) (Proteintech, 15204-1-AP), mouse monoclonal anti-DUSP5 (1:200) (Santa Cruz Biotechnology Inc., Dallas, TX, USA sc-393801), mouse monoclonal anti-p-ERK (Santa Cruz Biotechnology Inc., sc-7383), rabbit polyclonal anti-ERK1 (Santa Cruz Biotechnology Inc., sc-93), rabbit polyclonal anti-ERK2 (Santa Cruz Biotechnology Inc., sc-154), rabbit monoclonal anti-Mcl1 (1:1000) (Cell Signaling, 39224), mouse monoclonal anti-p53 (1:100) (clone DO-1, Santa Cruz Biotechnology Inc., sc-126). Mouse monoclonal anti-β-actin (1:10,000) (Sigma Aldrich) was used as the loading control. The goat antimouse IgG-HRP (1:30,000) (Bethyl Laboratories, A90-116P) and goat antirabbit IgG-HRP (1:30,000) (Bethyl Laboratories, A120-101P) were used as secondary antibodies. All primary and secondary antibodies were diluted in PBS–0.1% Tween20 solution containing 3% of BSA (SERVA).

Benedetti R., Gilardini Montani M.S., Romeo M.A., Arena A., Santarelli R., D’Orazi G, & Cirone M. (2021). Role of UPR Sensor Activation in Cell Death–Survival Decision of Colon Cancer Cells Stressed by DPE Treatment. Biomedicines, 9(9), 1262.

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Protein Expression Evaluation Protocol

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To evaluate the expression of proteins, we used the following antibodies: mouse monoclonal anti-PARP1 (1:100) (Santa Cruz Biotechnology Inc.), mouse monoclonal anti-p53 (1:100) (clone DO-1, Santa Cruz Biotechnology Inc.), mouse monoclonal anti-p21 (1:100) (Santa Cruz Biotechnology Inc.), mouse monoclonal anti-MVK (1:100) (Santa Cruz Biotechnology Inc.), mouse monoclonal anti-pERK (1:200) (Santa Cruz Biotechnology Inc.), rabbit polyclonal anti-ERK1 and anti-ERK2 (1:200) (Santa Cruz Biotechnology Inc.), rabbit polyclonal anti-BIP (1:1000) (Cell Signaling) and mouse monoclonal anti-CHOP (1:100) (Santa Cruz Biotechnology Inc.). Mouse monoclonal anti-β-actin (1:10,000) (Novus Biological) was used as loading control. The goat anti-mouse IgG-Horseradish Peroxidase (Santa Cruz Biotechnology Inc.) and goat anti-rabbit IgG-HRP (Santa Cruz Biotechnology Inc.) were used as secondary antibodies. All the primary and secondary antibodies were diluted in PBS—0.1% Tween20 solution containing 3% of BSA (SERVA).

Romeo M.A., Gilardini Montani M.S., Benedetti R., Garufi A., D’Orazi G, & Cirone M. (2020). PBA Preferentially Impairs Cell Survival of Glioblastomas Carrying mutp53 by Reducing Its Expression Level, Stabilizing wtp53, Downregulating the Mevalonate Kinase and Dysregulating UPR. Biomolecules, 10(4), 586.

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