Nanodrop 2000 2000c operating software

Manufactured by Thermo Fisher Scientific

Sourced in United States

The NanoDrop 2000/2000c Operating Software is a software application used to operate the NanoDrop 2000 and NanoDrop 2000c spectrophotometer instruments. The software provides the user interface and functionality to measure the absorbance of liquid samples and obtain concentration and purity measurements.

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Lab products found in correlation

Mirvanatm mirna isolation kit, by Thermo Fisher Scientific (2 mentions) Nanodroptm 2000, by Thermo Fisher Scientific (2 mentions) Rneasy ffpe kit, by Qiagen (2 mentions)

2 protocols using nanodrop 2000 2000c operating software

miRNA Extraction from Tissue Samples

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The miRNA content from the aorta, the liver and the cells were extracted following the mirVana^TM miRNA Isolation Kit (Invitrogen^TM, Thermo Fisher Scientific, Waltham, MA, USA). The miRNA content from paraffin-embedded carotids was extracted using the RNeasy FFPE kit (Qiagen, Hilden, Germany). All the extractions were made following the protocol handled by the manufacturers. In all cases, miRNAs and long RNAs were obtained in separate fractions. The miRNA sample concentration was then determined using a NanoDrop^TM 2000 and the NanoDrop 2000/2000c Operating Software (Thermo Scientific, Waltham, MA, USA).

González-López P., Ares-Carral C., López-Pastor A.R., Infante-Menéndez J., González Illaness T., Vega de Ceniga M., Esparza L., Beneit N., Martín-Ventura J.L., Escribano Ó, & Gómez-Hernández A. (2022). Implication of miR-155-5p and miR-143-3p in the Vascular Insulin Resistance and Instability of Human and Experimental Atherosclerotic Plaque. International Journal of Molecular Sciences, 23(18), 10253.

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miRNA Extraction from Aorta and Carotids

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The miRNA content from the aorta and the cells were extracted following the mirVanaTM miRNA Isolation Kit (InvitrogenTM, Thermo Fisher Scientific, Waltham, MA, USA). The miRNA content from paraffin‐embedded carotids was extracted using the RNeasy FFPE kit (Qiagen, Hilden, Germany). All the extractions were made following the protocol handled by the manufacturers. The mirVana^™ kit allows the isolation of miRNAs and long RNAs in separate fractions by differential precipitation. The miRNA sample concentration was determined using a NanoDropTM 2000 and the NanoDrop 2000/2000c Operating Software (Thermo Scientific, Waltham, MA, USA).

González‐López P., Álvarez‐Villarreal M., Ruiz‐Simón R., López‐Pastor A.R., de Ceniga M.V., Esparza L., Martín‐Ventura J.L., Escribano Ó, & Gómez‐Hernández A. (2023). Role of miR‐15a‐5p and miR‐199a‐3p in the inflammatory pathway regulated by NF‐κB in experimental and human atherosclerosis. Clinical and Translational Medicine, 13(8), e1363.

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