Log In Sign up
The largest database of trusted experimental protocols

Eif2α 9722

Manufactured by Cell Signaling Technology
Visit Supplier
Sourced in United States

The EIF2α (9722S) is a rabbit monoclonal antibody that recognizes endogenous levels of total eukaryotic translation initiation factor 2α (eIF2α) protein. It is suitable for use in Western blotting applications.

Automatically generated - may contain errors

Lab products found in correlation

Alexa fluor 488 goat anti rabbit igg a11034, by Thermo Fisher Scientific (2 mentions) E cadherin 3195, by Cell Signaling Technology (2 mentions) Vimentin 5741, by Cell Signaling Technology (2 mentions) Bip 3183, by Cell Signaling Technology (2 mentions) Gapdh fl 335, by Santa Cruz Biotechnology (2 mentions) β catenin, by Cell Signaling Technology (2 mentions) Alexa fluor 568 phalloidin, by Thermo Fisher Scientific (2 mentions) Zeb1 3396, by Cell Signaling Technology (2 mentions) Tubulin b512, by Merck Group (2 mentions) Snail, by Cell Signaling Technology (2 mentions) P eif2α, by Cell Signaling Technology (2 mentions) Hrp conjugated anti rabbit igg, by Cell Signaling Technology (1 mentions) Doxycycline, by Fujifilm (1 mentions) Cleaved parp 5625, by Cell Signaling Technology (1 mentions) Dimethyl sulfoxide (dmso), by Merck Group (1 mentions) Methyl thiazolyl tetrazolium (mtt), by Merck Group (1 mentions) Propidium iodide, by BD (1 mentions) N acetyl cysteine (nac), by Merck Group (1 mentions) Bcl2 sc 492, by Santa Cruz Biotechnology (1 mentions) Horseradish peroxidase hrp conjugated secondary antibody, by Santa Cruz Biotechnology (1 mentions)

Spelling variants of this product

EIF2α (268 citations)

6 protocols using eif2α 9722

1

Mitochondrial Stress Response Regulation

Check if the same lab product or an alternative is used in the 5 most similar protocols
CAP, doxycycline, and actinonin were obtained from Wako Pure Chemicals, Osaka, Japan. Antibodies against ATF4, p-eIF2α Ser51 (3795S), and eIF2α (9722S) were purchased from Cell Signaling Technology (Danvers, MA, U.S.A.). Anti-COXI (Ab14705) was from Abcam, Cambridge, U.K. COXIII and SDHA antibodies were from Thermo Fisher Scientific, Waltham, MA, U.S.A. The secondary antibodies, anti-mouse horseradish peroxidase (HRP)-conjugated IgG (#7076) and anti-rabbit HRP-conjugated IgG (#7074), were from Cell Signaling Technology.
Sasaki K., Uchiumi T., Toshima T., Yagi M., Do Y., Hirai H., Igami K., Gotoh K, & Kang D. (2020). Mitochondrial translation inhibition triggers ATF4 activation, leading to integrated stress response but not to mitochondrial unfolded protein response. Bioscience Reports, 40(11), BSR20201289.
+ Open protocol
+ Expand
2

Apoptosis Assay Protocol with Inhibitors

Check if the same lab product or an alternative is used in the 5 most similar protocols
CHE was obtained from Aladdin (Shanghai, China). Antibodies against B-cell lymphoma 2 (Bcl2, sc-492), Bcl2-associated protein x (Bax, sc-493), GAPDH (sc-32233), and horseradish peroxidase (HRP)-conjugated secondary antibodies were purchased from Santa Cruz Biotechnology (Santa Cruz, CA, USA). Antibodies against cleaved form of poly (ADP-ribose) polymerase (cleaved-PARP, 5625S), phosphorylated eukaryotic initiation factor 2α (p-eIF2α, 3398S), eIF2α (9722S), and activating transcription factor-4 (ATF4, 11815S) were obtained from Cell Signaling Technology (Danvers, MA, USA). NAC, DMSO, and MTT were purchased from Sigma-Aldrich (St Louis, MO, USA). Fluorescein isothiocyanate (FITC) Annexin V Apoptosis Detection Kit I and propidium iodide (PI) were purchased from BD Pharmingen (Franklin Lakes, NJ, USA). ROS probe 2′7′-dichlorodihydrofluorescein diacetate (DCFH-DA) was purchased from Thermo Fisher Scientific (Carlsbad, CA, USA).
Wu S., Yang Y., Li F., Huang L., Han Z., Wang G., Yu H, & Li H. (2018). Chelerythrine induced cell death through ROS-dependent ER stress in human prostate cancer cells. OncoTargets and therapy, 11, 2593-2601.
+ Open protocol
+ Expand
3

Antibody Utilization for Protein Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols
The following antibodies were used in this study. Antibodies for phospho-p38 (Thr180/Tyr182, 9211), p38 (9212), phospho-JNK (Thr183/Tyr185, 4668), β-actin (51255), phospho-mTOR (Ser2448, 2971) and eIF2α (9722S) were from Cell Signaling Technology. Antibodies for ZAKα (A301–933A) and RPS2 (A303–794A) were purchased from Bethyl Laboratories Inc. Phospho- eIF2α (Ser51, ab32157) from Abcam. Antibodies for FLAG (A8592) and ZAKP (HPA017205) were from Sigma-Aldrich. RPL4 antibody (11302–1-AP) was from Proteintech. GFP antibody (632381) was from Takara. Mouse anti-rabbit IgG-HRP (sc-2357) was from Santa Cruz Biotechnology. Goat anti-mouse IgG2a-HRP (115–035-2006) was from Jackson ImmunoResearch Laboratories Inc.
Wu C.C., Peterson A., Zinshteyn B., Regot S, & Green R. (2020). Ribosome collisions trigger general stress responses to regulate cell fate. Cell, 182(2), 404-416.e14.
+ Open protocol
+ Expand
4

Epithelial-Mesenchymal Transition Markers

Check if the same lab product or an alternative is used in the 5 most similar protocols
E-cadherin #3195, Vimentin #5741, ZEB1 #3396, Claudin1 #4933, Snail #3879, β-catenin #9562, Bip #3183, CHOP #2895, eIF2α #9722 and p-eIF2α #9721 (Cell Signaling Technologies Inc. Danvers, MA 01923); Tubulin #B512 (Sigma); GAPDH #FL335 (Santa Cruz); Ubqln polyclonal was made by inoculating rabbits with a peptide specific to Ubqln1 (Yenzym Antibodies LLC); Alexa Fluor 488 goat anti-rabbit IgG #A11034 (Molecular Probes, Invitrogen detection technologies, Eugene, OR. USA); Alexa Fluor 568 Phalloidin #A12380 (Life technologies Eugene, OR. USA).
Shah P.P., Lockwood W.W., Saurabh K., Kurlawala Z., Shannon S.P., Waigel S., Zacharias W, & Beverly L.J. (2014). Ubiquilin1 Represses Migration and Epithelial to Mesenchymal Transition of Human Non-small Cell Lung Cancer Cells. Oncogene, 34(13), 1709-1717.
+ Open protocol
+ Expand
5

Protein Expression Analysis by Western Blot

Check if the same lab product or an alternative is used in the 5 most similar protocols
Cells were lysed with lysis buffer containing 50 mM Tris-HCl, 1% sodium dodecyl sulfate, 1 mM dithiothreitol and 0.43 mM 4-(2-Aminoethyl) benzenesulfonyl fluoride hydrochloride. The lysates were sonicated and centrifuged at 20,400× g at 4 °C for 20 min, and the supernatant was collected. Equal amounts of the protein extract were subjected to sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and transferred to a polyvinylidene difluoride membrane (EMD Millipore, Billerica, MA, USA). The following were used as the primary antibodies: rabbit anti-human S6K (#2708), phospho-S6K (Thr389) (#9234), anti-human 4E-BP1 (#9644), phospho-4E-BP1 (Ser65) (#9451), ATF4 (#11815), ASCT2 (#8057), phospho-eIF2α (Ser51) (#9721), eIF2α (#9722) antibodies (Cell Signaling Technology, Inc., Danvers, MA, USA), mouse anti-human β-actin antibody (A5441; Sigma), mouse anti-human ASNS antibody (G-10; Santa Cruz Biotechnology, Santa Cruz, CA), rabbit anti-human GPT2 (16757-1-AP) antibody (Proteintech Group, Inc., Chicago, IL, USA), rabbit anti-human Glutaminase (ab156876) and xCT(ab37185) antibodies (Abcam, Cambridge, UK). Signals were detected with Chemi-Lumi One L (Nacalai Tesque, Kyoto, Japan) or Immobilon Western Chemiluminescent HRP Substrate (EMD Millipore). All experiments shown were replicated at least twice.
Boku S., Watanabe M., Sukeno M., Yaoi T., Hirota K., Iizuka-Ohashi M., Itoh K, & Sakai T. (2020). Deactivation of Glutaminolysis Sensitizes PIK3CA-Mutated Colorectal Cancer Cells to Aspirin-Induced Growth Inhibition. Cancers, 12(5), 1097.
+ Open protocol
+ Expand
6

Epithelial-Mesenchymal Transition Markers

Check if the same lab product or an alternative is used in the 5 most similar protocols
E-cadherin #3195, Vimentin #5741, ZEB1 #3396, Claudin1 #4933, Snail #3879, β-catenin #9562, Bip #3183, CHOP #2895, eIF2α #9722 and p-eIF2α #9721 (Cell Signaling Technologies Inc. Danvers, MA 01923); Tubulin #B512 (Sigma); GAPDH #FL335 (Santa Cruz); Ubqln polyclonal was made by inoculating rabbits with a peptide specific to Ubqln1 (Yenzym Antibodies LLC); Alexa Fluor 488 goat anti-rabbit IgG #A11034 (Molecular Probes, Invitrogen detection technologies, Eugene, OR. USA); Alexa Fluor 568 Phalloidin #A12380 (Life technologies Eugene, OR. USA).
Shah P.P., Lockwood W.W., Saurabh K., Kurlawala Z., Shannon S.P., Waigel S., Zacharias W, & Beverly L.J. (2014). Ubiquilin1 Represses Migration and Epithelial to Mesenchymal Transition of Human Non-small Cell Lung Cancer Cells. Oncogene, 34(13), 1709-1717.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!